3.1.26.4: ribonuclease H

This is an abbreviated version!
For detailed information about ribonuclease H, go to the full flat file.

Reaction

Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Ape-RNase HII, aRNase HII, Bh ribonuclease H, BhRNase H, CtepRNH, EcRNH, endoribonuclease H, endoribonuclease H (calf thymus), ERNH, huRNaseH1, hybrid nuclease, hybrid ribonuclease, hybridase, hybridase (ribonuclease H), KOD1, LC9-RNase H1, lmo1273, LRNase HII, More, nuclease, hybrid ribo-, nuclease, ribo-, H, P32, protein ST0753, reverse transcriptase, ribonuclease H, ribonuclease H I, ribonuclease H(42), ribonuclease H(70), ribonuclease H1, ribonuclease H2, ribonuclease H3, ribonuclease HI, ribonuclease HII, ribonuclease HIII, ribonulease H1, RNA*DNA hybrid ribonucleotidohydrolase, RNA*DNA hybrid ribonucleotiohydrolase, RNase H, RNase H type 2, RNase H(35), RNase H1, RNase H2, RNase H3, RNase HI, RNase HII, RNase HIII, RNaseH, Rnaseh1, RNASEH2A, RNH, RNH1, RNH2, rnhA, RnhB, rnhC, rnhD, Rv2228c, ST0519, STK_07530, Sto-RNase HI, T4 RNase H, Tk-RNase HII, Tk-Tk-RNase HII, TK0805, TRNH, TthRNH, Ty1 reverse transcriptase/RNase H, type 1 ribonuclease H, type 1 RNase H, type 2 ribonuclease H, type 2 RNase H, type II ribonuclease H

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.26 Endoribonucleases producing 5'-phosphomonoesters

                3.1.26.4 ribonuclease H

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Results	in table
16	Activating Compound
117230	AA Sequence
12	Application
1	CAS Registry Number
64	Cloned(Commentary)
35	Crystallization (Commentary)
18003	Disease/ Diagnostics
79	General Information
10	General Stability
24	IC50 Value
171	Inhibitors
10	kcat/KM [mM/s]
2	Ki Value [mM]
87	KM Value [mM]
39	Localization
233	Metals/Ions
71	Molecular Weight [Da]
69	Natural Substrates/ Products (Substrates)
441	Organism
470	PDB ID
83	pH Optimum
16	pH Range
2	pH Stability
2	pI Value
2	Posttranslational Modification
280	Protein Variants
76	Purification (Commentary)
20	Reaction
2	Reaction Type
219	Reference
6	Renatured (Commentary)
58	Source Tissue
67	Specific Activity [micromol/min/mg]
4	Storage Stability
332	Substrates and Products (Substrate)
61	Subunits
315	Synonyms
53	Temperature Optimum [°C]
6	Temperature Range [°C]
41	Temperature Stability [°C]
49	Turnover Number [1/s]

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

-

Escherichia coli

-

134080

comparison of the folding trajectories of the three-state RNase H mutant D10A and the two-state RNase H mutant D10A/I53D, proteins with the same native-state topology but altered regional stability. Both versions of RNase H fold through a similar trajectory with similar high-energy conformations. Mutations in the core and the periphery of the protein affect similar aspects of folding for both variants

Escherichia coli

-

709522

recombinant I53 and L56 point mutants after being expressed insolubly in inclusion bodies in Escherichia coli

Chlorobaculum tepidum

-

714194

refolding from purified urea-denatured form with low yield

Escherichia coli

-

655904

study on thermal and mechanical folding and unfolding mechanisms. Mechanical and thermal unfolding proceed through three stable states that are similar. The one difference between the two regimes is in the transition occurring at the most denaturing conditions. For the thermal case, both secondary and tertiary structures melt. For the mechanical case, the secondary structure of the helices remains largely intact, while the bundle itself unfolds. Of the major secondary structure motifs, alpha-helices are more stable than beta-sheets

Escherichia coli

-

709259

the denaturation by guanidinium chloride is completely reversible at 50°C, the unfolding and refolding reactions of the enzyme attain a two-state equilibrium in 2 weeks

Thermococcus kodakarensis

O74035

747059