Literature summary for 3.1.26.4 extracted from

Chon, H.; Vassilev, A.; DePamphilis, M.L.; Zhao, Y.; Zhang, J.; Burgers, P.M.; Crouch, R.J.; Cerritelli, S.M.
Contributions of the two accessory subunits, RNASEH2B and RNASEH2C, to the activity and properties of the human RNase H2 complex (2009), Nucleic Acids Res., 37, 96-110.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and in HeLa cell	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A177T	mutation in subunit H2B associated with Aicardi-Goutieres' syndrome, near-normal activity	Homo sapiens
G185V	mutation in subunit H2B associated with Aicardi-Goutieres' syndrome, near-normal activity	Homo sapiens
G37S	mutation in subunit H2A associated with Aicardi-Goutieres' syndrome, greatly reduced activity and processivity	Homo sapiens
K143I	mutation in subunit H2C associated with Aicardi-Goutieres' syndrome, near-normal activity	Homo sapiens
K162T	mutation in subunit H2B associated with Aicardi-Goutieres' syndrome, near-normal activity	Homo sapiens
R69W	mutation in subunit H2C associated with Aicardi-Goutieres' syndrome, 30-40% of wild-type activity	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	highest activity in presence of 5-10 mM	Homo sapiens
Mn2+	0.1-1 mM, 20-30% of maximum activity	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isoform RNase H2	-
Homo sapiens	Q5TBB1	subunit H2B; isoform RNase H2	-
Homo sapiens	Q8TDP1	subunit H2C; isoform RNase H2	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA12-RNA1-DNA27/DNA40 hybrid + H2O	-	Homo sapiens	?	enzyme cleaves RNA20/DNA20 hybrid and DNA12-RNA1-DNA27/DNA40 hybrid substrates with similar efficiency	?
poly-rA/poly-dT + H2O	-	Homo sapiens	?	products are short oligonucleotides with very few intermediate-sized oligonucleotides	?
RNA20/DNA20 hybrid + H2O	-	Homo sapiens	?	enzyme cleaves RNA20/DNA20 hybrid and DNA12-RNA1-DNA27/DNA40 hybrid substrates with similar efficiency	?

Subunits

Subunits	Comment	Organism
trimer	heterotrimer of subunits H2A, H2B, H2C, SDS-PAGE and gel filtration	Homo sapiens

Synonyms

Synonyms	Comment	Organism
RNase H2	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.2	8.8	-	Homo sapiens

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Release 2023.1 (January 2023)