3.1.26.4: ribonuclease H

This is an abbreviated version!
For detailed information about ribonuclease H, go to the full flat file.

Reaction

Endonucleolytic cleavage to a 5'-phosphomonoester =

Synonyms

Ape-RNase HII, aRNase HII, Bh ribonuclease H, BhRNase H, CtepRNH, EcRNH, endoribonuclease H, endoribonuclease H (calf thymus), ERNH, huRNaseH1, hybrid nuclease, hybrid ribonuclease, hybridase, hybridase (ribonuclease H), KOD1, LC9-RNase H1, lmo1273, LRNase HII, More, nuclease, hybrid ribo-, nuclease, ribo-, H, P32, protein ST0753, reverse transcriptase, ribonuclease H, ribonuclease H I, ribonuclease H(42), ribonuclease H(70), ribonuclease H1, ribonuclease H2, ribonuclease H3, ribonuclease HI, ribonuclease HII, ribonuclease HIII, ribonulease H1, RNA*DNA hybrid ribonucleotidohydrolase, RNA*DNA hybrid ribonucleotiohydrolase, RNase H, RNase H type 2, RNase H(35), RNase H1, RNase H2, RNase H3, RNase HI, RNase HII, RNase HIII, RNaseH, Rnaseh1, RNASEH2A, RNH, RNH1, RNH2, rnhA, RnhB, rnhC, rnhD, Rv2228c, ST0519, STK_07530, Sto-RNase HI, T4 RNase H, Tk-RNase HII, Tk-Tk-RNase HII, TK0805, TRNH, TthRNH, Ty1 reverse transcriptase/RNase H, type 1 ribonuclease H, type 1 RNase H, type 2 ribonuclease H, type 2 RNase H, type II ribonuclease H

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.26 Endoribonucleases producing 5'-phosphomonoesters

                3.1.26.4 ribonuclease H

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Results	in table
16	Activating Compound
117230	AA Sequence
12	Application
1	CAS Registry Number
64	Cloned(Commentary)
35	Crystallization (Commentary)
18003	Disease/ Diagnostics
79	General Information
10	General Stability
24	IC50 Value
171	Inhibitors
10	kcat/KM [mM/s]
2	Ki Value [mM]
87	KM Value [mM]
39	Localization
233	Metals/Ions
71	Molecular Weight [Da]
69	Natural Substrates/ Products (Substrates)
441	Organism
470	PDB ID
83	pH Optimum
16	pH Range
2	pH Stability
2	pI Value
2	Posttranslational Modification
280	Protein Variants
76	Purification (Commentary)
20	Reaction
2	Reaction Type
219	Reference
6	Renatured (Commentary)
58	Source Tissue
67	Specific Activity [micromol/min/mg]
4	Storage Stability
332	Substrates and Products (Substrate)
61	Subunits
315	Synonyms
53	Temperature Optimum [°C]
6	Temperature Range [°C]
41	Temperature Stability [°C]
49	Turnover Number [1/s]

IC50 Value

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IC50 Value on EC 3.1.26.4 - ribonuclease H

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IC50 VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.00019

2-(2,3-dimethylphenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.00045

2-(2,5-dimethylphenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.00833

2-(4,6-dimethyl-3-oxo-1,2-benzothiazol-2(3H)-yl)-N-propylacetamide

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.0005

2-(4-chlorophenyl)-6-fluoro-1,2-benzothiazol-3(2H)-one

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.00005

2-(6-fluoro-3-oxo-1,2-benzothiazol-2(3H)-yl)-N-(4-methylphenyl)acetamide

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.00002

2-phenyl-1,2-benzothiazol-3(2H)-one

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.0019

2-[(cyclopentylcarbonyl)amino]-4-ethyl-5-methylthiophene-3-carboxamide

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.000357 - 0.000402

4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one

2 entries

0.05

5-nitrofuran-2-carboxylic acid [[4-(4-bromophenyl)-thiazol-2-yl]-(tetrahydrofuran-2-ylmethyl)-carbamoyl] methyl ester

Homo sapiens

-

inhibitory activity against human RNase H1. IC50 value about 50 microM

693579

0.0064

6-(naphthalen-2-yl)-3-(pyridin-3-yl)[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.00001

6-fluoro-2-(2-methylphenyl)-1,2-benzothiazol-3(2H)-one

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.0002

6-fluoro-2-(4-methylphenyl)-1,2-benzothiazol-3(2H)-one

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.01

7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione

2 entries

0.0054

N-(5-benzyl-1,3,4-thiadiazol-2-yl)-2-ethylhexanamide

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.00048

N-benzyl-2-(6-fluoro-3-oxo-1,2-benzothiazol-2(3H)-yl)acetamide

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.00332

N-cyclopentyl-2-(4,6-dimethyl-3-oxo[1,2]thiazolo[5,4-b]pyridin-2(3H)-yl)acetamide

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.0004

N-cyclopropyl-1-methyl-3-oxo-1,3-dihydro-2,1-benzothiazole-5-sulfonamide

Homo sapiens

-

60 mM KCl, 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.01% (w/v) bovine serum albumin, 0.01% (v/v) Triton X-100, at 20°C

730068

0.01

trihydroxy benzoyl biphenyl carboxylate hydrazone

2 entries

0.01

trihydroxybenzoyl naphthyl hydrazone

2 entries

additional information

additional information

Homo sapiens

-

no inhibitory activity against human RNase H1 for 5-nitrofuran-2-carboxylic acid adamantan-1-carbamoyl methyl ester

693579

-

0.000357

4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one

Xenotropic MuLV-related virus

A1Z651

with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C

728974

0.000402

4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one

Xenotropic MuLV-related virus

A1Z651

with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C

728974

0.01

7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione

Xenotropic MuLV-related virus

A1Z651

IC50 above 0.01 mM, with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C

728974

0.01

7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione

Xenotropic MuLV-related virus

A1Z651

IC50 above 0.01 mM, with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C

728974

0.01

trihydroxy benzoyl biphenyl carboxylate hydrazone

Xenotropic MuLV-related virus

A1Z651

IC50 above 0.01 mM, with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C

728974

0.01

trihydroxy benzoyl biphenyl carboxylate hydrazone

Xenotropic MuLV-related virus

A1Z651

IC50 above 0.01 mM, with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C

728974

0.01

trihydroxybenzoyl naphthyl hydrazone

Xenotropic MuLV-related virus

A1Z651

IC50 above 0.01 mM, with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C

728974

0.01

trihydroxybenzoyl naphthyl hydrazone

Xenotropic MuLV-related virus

A1Z651

IC50 above 0.01 mM, with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C

728974