Cloned (Comment) | Organism |
---|---|
overexpression of N-terminally His-tagged RNase HIII in Escherichia coli strain BL21 CodonPlus (DE3) RIPL | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme Sa-RNase HIII, sitting drop vapour diffusion method, 0.004 ml of 3.2 mg/ml protein in PBS containing 137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate dibasic, 2 mM potassium phosphate monobasic, pH 7.4, is mixed with 0.002 ml of precipitation solution containing 0.17 M ammonium sulfate, 25.5% w/v PEG 8000, 15% v/v glycerol, and 0.085 M sodium cacodylate pH 6.5, X-ray diffraction structure determination and analysis at 2.59-2.70 A resolution | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+ | Staphylococcus aureus | |
Mn2+ | RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+ | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Staphylococcus aureus | RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+ | ? | - |
? | |
additional information | Staphylococcus aureus MRSA252 | RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+ | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus MRSA252 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged RNase HIII from Escherichia coli strain BL21 CodonPlus (DE3) RIPL by nickel affinity chromatography, dialysis and cleavage of the His-tag by TEV protease | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+ | Staphylococcus aureus | ? | - |
? | |
additional information | RNases hydrolyze RNA/DNA in the presence of various divalent cofactors such as Mg2+ and Mn2+ | Staphylococcus aureus MRSA252 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | RNase HIII-type ribonucleases are members of the RNase H group of endonucleases | Staphylococcus aureus |
RNase HIII | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
physiological function | RNase HIII-type ribonucleases are members of the RNase H group of endonucleases which hydrolyze RNA from RNA/DNA hybrids and are possibly be involved in DNA replication and repair | Staphylococcus aureus |