Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D39A | inactive | Saccharomyces cerevisiae |
G42S | the mutant shows strongly reduced activity compared to the wild type enzyme | Saccharomyces cerevisiae |
P45D/Y219A | the mutant shows strongly reduced activity compared to the wild type enzyme | Saccharomyces cerevisiae |
P45E/Y219A | inactive | Saccharomyces cerevisiae |
P45S/Y219A | inactive | Saccharomyces cerevisiae |
Y219A | inactive | Saccharomyces cerevisiae |
Y219D | inactive | Saccharomyces cerevisiae |
Y219E | inactive | Saccharomyces cerevisiae |
Y219S | inactive | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | Saccharomyces cerevisiae | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? | |
RNA-DNA hybrid + H2O | Saccharomyces cerevisiae W-303 | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae W-303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap heparin column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | - |
Saccharomyces cerevisiae | ribonucleotide 5'-phosphomonoester + ? | - |
? | |
RNA-DNA hybrid + H2O | - |
Saccharomyces cerevisiae W-303 | ribonucleotide 5'-phosphomonoester + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease H2 | - |
Saccharomyces cerevisiae |
RNase H2 | - |
Saccharomyces cerevisiae |