Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
three-dimensional structural model of RNase HII complexed with its substrate, suggests that the amino acids D110, R113 and F114 are located in the region that discriminates DNA from RNA in the non-substrate strand of RNA-RNA | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
A115E | partially enhanced nuclease activity for the RNA-DNA duplex substrate | Pyrococcus furiosus |
F114E | reduced nuclease activity respectively for the RNA-DNA duplex substrate | Pyrococcus furiosus |
R113E | partially reduced nuclease activity respectively for the RNA-DNA duplex substrate | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
RNA-DNA duplex | mutant F114E, 50°C, presence of Mn2+ | Pyrococcus furiosus | |
0.00012 | - |
RNA-DNA duplex | wild-type, 50°C, presence of Mg2+ | Pyrococcus furiosus | |
0.00012 | - |
RNA-DNA duplex | wild-type, 50°C, presence of Mn2+ | Pyrococcus furiosus | |
0.00014 | - |
RNA-DNA duplex | mutant A115E, 50°C, presence of Mn2+ | Pyrococcus furiosus | |
0.0002 | - |
RNA-DNA duplex | mutant R113E, 50°C, presence of Mn2+ | Pyrococcus furiosus | |
0.0013 | - |
RNA-RNA duplex | wild-type, 50°C, presence of Mn2+ | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | wild-type digests RNA-RNA duplexes in presence of Mn2+ | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, SDS-PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U036 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA duplex + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
RNA-RNA duplex + H2O | reaction occurs in presence of Mn2+-ions. Residues D110, R113 and F114 are responsible for the activity, the residues are located in the region that discriminates DNA from RNA in the non-substrate strand of the duplexes | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
RNA-RNA duplex | wild-type, 50°C, presence of Mn2+ | Pyrococcus furiosus | |
0.0068 | - |
RNA-DNA duplex | mutant F114E, 50°C, presence of Mn2+ | Pyrococcus furiosus | |
0.11 | - |
RNA-DNA duplex | mutant R113E, 50°C, presence of Mn2+ | Pyrococcus furiosus | |
0.188 | - |
RNA-DNA duplex | wild-type, 50°C, presence of Mn2+ | Pyrococcus furiosus | |
0.255 | - |
RNA-DNA duplex | mutant A115E, 50°C, presence of Mn2+ | Pyrococcus furiosus | |
0.283 | - |
RNA-DNA duplex | wild-type, 50°C, presence of Mg2+ | Pyrococcus furiosus |