3.1.21.7: deoxyribonuclease V
This is an abbreviated version!
For detailed information about deoxyribonuclease V, go to the full flat file.
Word Map on EC 3.1.21.7
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3.1.21.7
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nick
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deaminate
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phosphodiester
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beta-polymerase
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deoxyinosine-containing
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3\'-hydroxyl
-
proofreading
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biotechnology
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analysis
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molecular biology
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drug development
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medicine
- 3.1.21.7
- nick
-
deaminate
-
phosphodiester
-
beta-polymerase
-
deoxyinosine-containing
-
3\'-hydroxyl
-
proofreading
- biotechnology
- analysis
- molecular biology
- drug development
- medicine
Reaction
endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate =
Synonyms
AGTendoV, deoxyinosine 3' endonuclease, Deoxyinosine 3'endonuclease, DNase V, EC 3.1.22.3, endo V, endodeoxyribonuclease V, endonuclease V, EndoV, Escherichia coli endodeoxyribonuclease V, nfi, PF0987, PfuEndoV, T4 endonuclease V, T4 UV endonuclease, Tb10.6k15.3890
ECTree
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Reference on EC 3.1.21.7 - deoxyribonuclease V
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nyaga, S.G.; Dodson, M.L.; Lloyd, R.S.
Role of specific amino acid residues in T4 endonuclease V that alter nontarget DNA binding
Biochemistry
36
4080-4088
1997
Tequatrovirus T4
McCullough, A.K.; Dodson, M.L.; Schärer, O.D.; Lloyd, R.S.
The role pf base flipping in damge recognition and catalysis by nT4 endonuclease V
J. Biol. Chem.
272
27210-27217
1997
Tequatrovirus T4
Vassylyev, D.G.; Kashiwagi, T.; Mikami, Y.; Ariyoshi, M.; Iwai, S.; Ohtsuka, E.; Morikawa, K.
Atomic model of a pyrmidine dimer excision repair enzyme complexed with a DNA substrate: structural basis for damaged DNA recognition
Cell
83
773-782
1995
Tequatrovirus T4
McCullough, A.K.; Schärer, O.; Verdine, G.L.; Lloyd, R.S.
Structural determinants for specific recognition by T4 endonuclease V
J. Biol. Chem.
271
32147-32152
1996
Tequatrovirus T4
Yao, M.; Kow, Y.W.
Further characterization of Escherichia coli endonuclease V. Mechanism of recognition for deoxyinosine, deoxyuridine, and base mismatches in DNA
J. Biol. Chem.
272
30774-30779
1997
Escherichia coli
Latham, K.A.; Rajendran, S.; Carmical, J.R.; Lee, J.C.; Lloyd, R.S.
T4 endonuclease V exists in solution as a monomer and binds to target sites as a monomer
Biochim. Biophys. Acta
1292
324-334
1996
Tequatrovirus T4
Latham, K.A.; Lloyd, R.S.
delta-Eliminiation by T4 endonuclease V at a thymine dimer site requires a secondary binding event and amino acid Glu-23
Biochemistry
34
8796-8803
1995
Tequatrovirus T4
Dodson, M.L.; Schrockk III, R.D.; Lloyd, R.S.
Evidence for an imino intermediate in the T4 endonuclease V reaction
Biochemistry
32
8284-8290
1993
Tequatrovirus T4
Sibghat-Ullah; Sancar, A.
In vitro characterization of repair synthesis initiated by T4 endonuclease V on a synthetic DNA substrate
Indian J. Biochem. Biophys.
29
227-230
1992
Tequatrovirus T4
Nickell, C.; Lloyd, R.S.
Mutations in endonuclease V that affect both protein-protein association and target site location
Biochemistry
30
8638-8648
1991
Tequatrovirus T4
Prince, M.A.; Friedman, B.; Gruskin, E.A.; Schrock III, R.D.; Lloyd, R.S.
Selective metal binding to Cys-78 within endonuclease V cause an inhibition of catalytic aactivities without altering nontarget and target DNA binding
J. Biol. Chem.
266
10686-10693
1991
Tequatrovirus T4
Morikawa, K.; Matsumoto, O.; Tsujimoto, M.; Katayanagi, K.; Ariyoshi, M.; Doi, T.; Ikehara, M.; Inaoka, T.; Ohtsuka, E.
X-ray structure of T4 endonuclease V: An excision repair enzyme specific for a pyrimidine dimer
Science
256
523-526
1992
Tequatrovirus T4
Kim, J.; Linn, S.
The mechanism of action of E. coli endonuclease III and T4 UV endonuclease (endonuclease V) at AP sites
Nucleic Acids Res.
16
1135-1141
1988
Tequatrovirus T4
Mosbaugh, D.W.; Linn, S.
Excision repair and DNA synthesis with a combination of HeLa DNA polymerase beta and DNase V
J. Biol. Chem.
258
108-118
1983
Homo sapiens
Demple, B.; Linn, S.
On the recognition and cleavage mechanism of Escherichia coli endodeoxyribonuclease V, a possible DNA repair enzyme
J. Biol. Chem.
257
2848-2855
1982
Escherichia coli
Demple, B.; Gates III, F.T.; Linn, S.
Purification and properties of Escherichia coli endodeoxyribonuclease V
Methods Enzymol.
65
224-231
1980
Escherichia coli
Wang, E.G.; Furth, J.J.
Mammalian endonuclease, DNase V. Purification and properties of enzyme of calf thymus
J. Biol. Chem.
252
116-124
1977
Bos taurus
Gates III, F.T.; Linn, S.
Endonuclease from Escherichia coli that acts specifically upon duplex DNA damaged by ultraviolet light, osmium tetroxide, acid, or x-rays
J. Biol. Chem.
252
2802-2807
1977
Escherichia coli
Kanugula, S.; Pauly, G.T.; Moschel, R.C.; Pegg, A.E.
A bifunctional DNA repair protein from Ferroplasma acidarmanus exhibits O6-alkylguanine-DNA alkyltransferase and endonuclease V activities
Proc. Natl. Acad. Sci. USA
102
3617-3622
2005
Ferroplasma acidarmanus
Feng, H.; Dong, L.; Klutz, A.M.; Aghaebrahim, N.; Cao, W.
Defining amino acid residues involved in DNA-protein interactions and revelation of 3'-exonuclease activity in endonuclease V
Biochemistry
44
11486-11495
2005
Thermotoga maritima
Feng, H.; Klutz, A.; Cao, W.
Active site plasticity of endonuclease V from Salmonella typhimurium
Biochemistry
44
675-683
2005
Salmonella enterica subsp. enterica serovar Typhimurium
Miyazaki, K.
Random DNA fragmentation with enonuclease V: application to DNA shuffling
Nucleic Acids Res.
30
e139
2002
Escherichia coli
Hitchcock, T.M.; Gao, H.; Cao, W.
Cleavage of deoxyoxanosine-containing oligodeoxyribonucleotides by bacterial endonuclease V
Nucleic Acids Res.
32
4071-4080
2004
Salmonella sp.
Doi, Y.; Katafuchi, A.; Fujiwara, Y.; Hitomi, K.; Tainer, J.A.; Ide, H.; Iwai, S.
Synthesis and characterization of oligonucleotides containing 2'-fluorinated thymidine glycol as inhibitors of the endonuclease III reaction
Nucleic Acids Res.
34
1540-1551
2006
Tequatrovirus T4
Turner, D.J.; Pingle, M.R.; Barany, F.
Harnessing asymmetrical substrate recognition by thermostable EndoV to achieve balanced linear amplification in multiplexed SNP typing
Biochem. Cell Biol.
84
232-242
2006
Thermotoga maritima
Feng, H.; Dong, L.; Cao, W.
Catalytic Mechanism of Endonuclease V: A Catalytic and Regulatory Two-Metal Model
Biochemistry
45
10251-10259
2006
Thermotoga maritima (Q9X2H9)
Lin, J.; Gao, H.; Schallhorn, K.A.; Harris, R.M.; Cao, W.; Ke, P.C.
Lesion Recognition and Cleavage by Endonuclease V: A Single-Molecule Study
Biochemistry
46
7132-7137
2007
Thermotoga maritima (Q9X2H9)
Wright, T.I.; Spencer, J.M.; Flowers, F.P.
Chemoprevention of nonmelanoma skin cancer
J. Am. Acad. Dermatol.
54; 933-46
quiz 947-50
2006
Tequatrovirus T4
Lhiaubet-Vallet, V.; Cuquerella, M.C.; Castell, J.V.; Bosca, F.; Miranda, M.A.
Triplet excited fluoroquinolones as mediators for thymine cyclobutane dimer formation in DNA
J. Phys. Chem. B
111
7409-7414
2007
Tequatrovirus T4
Gao, H.; Huang, J.; Barany, F.; Cao, W.
Switching base preferences of mismatch cleavage in endonuclease V: an improved method for scanning point mutations
Nucleic Acids Res.
35
e2/1-e2/6
2007
Thermotoga maritima (Q9X2H9)
-
Jiang, Y.; Rabbi, M.; Kim, M.; Ke, C.; Lee, W.; Clark, R.L.; Mieczkowski, P.A.; Marszalek, P.E.
UVA generates pyrimidine dimers in DNA directly
Biophys. J.
96
1151-1158
2009
Tequatrovirus T4
Dong, K.K.; Damaghi, N.; Picart, S.D.; Markova, N.G.; Obayashi, K.; Okano, Y.; Masaki, H.; Grether-Beck, S.; Krutmann, J.; Smiles, K.A.; Yarosh, D.B.
UV-induced DNA damage initiates release of MMP-1 in human skin
Exp. Dermatol.
17
1037-1044
2008
Tequatrovirus T4
Cafardi, J.A.; Elmets, C.A.
T4 endonuclease V: review and application to dermatology
Expert. Opin. Biol. Ther.
8
829-838
2008
Tequatrovirus T4
Majorek, K.A.; Bujnicki, J.M.
Modeling of Escherichia coli Endonuclease V structure in complex with DNA
J. Mol. Model.
15
173-182
2009
Escherichia coli
Dalhus, B.; Arvai, A.S.; Rosnes, I.; Olsen, O.E.; Backe, P.H.; Alseth, I.; Gao, H.; Cao, W.; Tainer, J.A.; Bjoras, M.
Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair
Nat. Struct. Mol. Biol.
16
138-143
2009
Thermotoga maritima (Q9X2H9)
Lee, C.; Yang, Y.; Goodman, S.; Yu, Y.; Lin, S.; Kao, J.; Tsai, K.; Fang, W.
Endonuclease V-mediated deoxyinosine excision repair in vitro
DNA Repair
9
1073-1079
2010
Escherichia coli
Mi, R.; Abole, A.; Cao, W.
Dissecting endonuclease and exonuclease activities in endonuclease V from Thermotoga maritima
Nucleic Acids Res.
39
536-544
2011
Thermotoga maritima
Kiyonari, S.; Egashira, Y.; Ishino, S.; Ishino, Y.
Biochemical characterization of endonuclease V from the hyperthermophilic archaeon, Pyrococcus furiosus
J. Biochem.
155
325-333
2014
Pyrococcus furiosus (Q8U263), Pyrococcus furiosus
Zhang, Z.; Hao, Z.; Wang, Z.; Li, Q.; Xie, W.
Structure of human endonuclease V as an inosine-specific ribonuclease
Acta Crystallogr. Sect. D
70
2286-2294
2014
Homo sapiens (Q8N8Q3)
Baumann, T.; Arndt, K.; Mueller, K.
Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V
BMC Biotechnol.
13
81
2013
Escherichia coli, Thermotoga maritima
Vik, E.S.; Nawaz, M.S.; Strom Andersen, P.; Fladeby, C.; Bjoras, M.; Dalhus, B.; Alseth, I.
Endonuclease V cleaves at inosines in RNA
Nat. Commun.
4
2271
2013
Escherichia coli, Homo sapiens (Q8N8Q3)
Fladeby, C.; Vik, E.S.; Laerdahl, J.K.; Gran Neurauter, C.; Heggelund, J.E.; Thorgaard, E.; Strom-Andersen, P.; Bjoras, M.; Dalhus, B.; Alseth, I.
The human homolog of Escherichia coli endonuclease V is a nucleolar protein with affinity for branched DNA structures
PLoS ONE
7
e47466
2012
Homo sapiens (Q8N8Q3)
Rosnes, I.; Rowe, A.D.; Vik, E.S.; Forstrom, R.J.; Alseth, I.; Bjoras, M.; Dalhus, B.
Structural basis of DNA loop recognition by endonuclease V
Structure
21
257-265
2013
Thermotoga maritima (Q9X2H9)
Ishino, S.; Makita, N.; Shiraishi, M.; Yamagami, T.; Ishino, Y.
EndoQ and EndoV work individually for damaged DNA base repair in Pyrococcus furiosus
Biochimie
118
264-269
2015
Pyrococcus furiosus (Q8U263)
Wang, W.; Peng, F.; Li, W.; Huang, Y.; Nie, Z.; Yao, S.
A dual enzymatic amplified strategy for the detection of endonuclease v activity
Anal. Methods
7
8453-8458
2015
Escherichia coli
-
Su, K.Y.; Lin, L.I.; Goodman, S.D.; Yen, R.S.; Wu, C.Y.; Chang, W.C.; Yang, Y.C.; Cheng, W.C.; Fang, W.H.
DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo
DNA Repair
64
59-67
2018
Escherichia coli (P68739), Escherichia coli, Escherichia coli K12 (P68739)
Kim, J.; Tohashi, K.; Iwai, S.; Kuraoka, I.
Inosine-specific ribonuclease activity of natural variants of human endonuclease V
FEBS Lett.
590
4354-4360
2016
Homo sapiens (Q8N8Q3)
-
Wang, Y.; Zhang, L.; Zhu, X.; Li, Y.; Shi, H.; Oger, P.; Yang, Z.
Biochemical characterization of a thermostable endonuclease V from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5
Int. J. Biol. Macromol.
117
17-24
2018
Thermococcus barophilus (A0A0S1X9V3), Thermococcus barophilus, Thermococcus barophilus Ch5 (A0A0S1X9V3)
Nawaz, M.S.; Vik, E.S.; Berges, N.; Fladeby, C.; Bjoras, M.; Dalhus, B.; Alseth, I.
Regulation of human endonuclease V activity and relocalization to cytoplasmic stress granules
J. Biol. Chem.
291
21786-21801
2016
Homo sapiens (Q8N8Q3)
Ahmadi, A.; Rosnes, I.; Blicher, P.; Diekmann, R.; Schuettpelz, M.; Glette, K.; Torresen, J.; Bjoras, M.; Dalhus, B.; Rowe, A.D.
Breaking the speed limit with multimode fast scanning of DNA by endonuclease V
Nat. Commun.
9
5381
2018
Thermotoga maritima (Q9X2H9), Thermotoga maritima ATCC 43589 (Q9X2H9)
Zhang, Z.; Jia, Q.; Zhou, C.; Xie, W.
Crystal structure of E. coli endonuclease V, an essential enzyme for deamination repair
Sci. Rep.
5
12754
2015
Escherichia coli (B7MIY4), Escherichia coli O45:K1/S88/ExPEC (B7MIY4), Escherichia coli O45:K1/S88 (B7MIY4)
Nawaz, M.S.; Vik, E.S.; Ronander, M.E.; Solvoll, A.M.; Blicher, P.; Bjoras, M.; Alseth, I.; Dalhus, B.
Crystal structure and MD simulation of mouse EndoV reveal wedge motif plasticity in this inosine-specific endonuclease
Sci. Rep.
6
24979
2016
Mus musculus (Q8C9A2)
Garcia-Caballero, D.; Perez-Moreno, G.; Estevez, A.M.; Ruiz-Perez, L.M.; Vidal, A.E.; Gonzalez-Pacanowska, D.
Insights into the role of endonuclease V in RNA metabolism in Trypanosoma brucei
Sci. Rep.
7
8505
2017
Trypanosoma brucei (Q38AS1), Trypanosoma brucei brucei (Q38AS1), Trypanosoma brucei 927 (Q38AS1), Trypanosoma brucei brucei 927/4 GUTat10.1 (Q38AS1)
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