Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
D35A | inactive mutant enzyme | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 200 mM, complete inhibition | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity is stimulated by the presence of Mg2+ or Mn2+ in the reaction buffer, with an optimum at 1 mM Mg2+ | Pyrococcus furiosus | |
Mn2+ | activity is stimulated by the presence of Mg2+ or Mn2+ in the reaction buffer, with an optimum at 1 mM Mg2+ | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Pyrococcus furiosus | endonuclease V recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion in damaged DNA. The enzyme possesses specific endonuclease activity for the deoxyinosine-containing DNA strand | ? | - |
? | |
nicked DNA + H2O | Pyrococcus furiosus | - |
? | - |
? | |
RNA + H2O | Pyrococcus furiosus | the enzyme also cleaves inosine-containing RNA strands | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U263 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | endonuclease V recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion in damaged DNA. The enzyme possesses specific endonuclease activity for the deoxyinosine-containing DNA strand | Pyrococcus furiosus | ? | - |
? | |
dsDNA containing deoxyinosine + H2O | no cleavage activity is detected with the dsDNA containing a T/G mismatch base pair and a dsDNA containing an apurinic site. The enzyme possesses strict substrate specificity to deoxyinosine-containing DNA. The enzyme cleaves inosine-containing RNA strands as well as DNA substrates | Pyrococcus furiosus | ? | - |
? | |
nicked DNA + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
RNA + H2O | the enzyme also cleaves inosine-containing RNA strands | Pyrococcus furiosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo V | - |
Pyrococcus furiosus |
endonuclease V | - |
Pyrococcus furiosus |
EndoV | - |
Pyrococcus furiosus |
PF0987 | locus name | Pyrococcus furiosus |
PfuEndoV | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
a moderate reaction temperature is used for the biochemical studies, because of the melting temperature of the DNA duplex substrates (Tm = 67ºC) | Pyrococcus furiosus |
90 | - |
- |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 100 | 60°C: about 45% of maximal activity, 100°C: about 70% of maximal activity | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.8 | pH 7.0: about 45% of maximal activity, pH 8.8: about 90% of maximal activity | Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
metabolism | this DNA repair enzyme is also involved in RNA metabolism | Pyrococcus furiosus |
physiological function | DNA repair enzyme, may also be involved in RNA metabolism | Pyrococcus furiosus |