Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
E23Q | no detection of base movement prior to catalysis | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Tequatrovirus T4 | pyrimidine dimer-specific | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? | |
DNA + H2O | Tequatrovirus T4 | moving a DNA base extrahelical opposite the target site that induces a conformational change to facilitate catalysis | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
49-mer oligonucleotide + H2O | also possesses abasic lyase activity | Tequatrovirus T4 | hydrolysed DNA | - |
? | |
49-mer oligonucleotide + H2O | double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | hydrolysed DNA | - |
? | |
DNA + H2O | abasic lyase activity | Tequatrovirus T4 | 3-alpha,beta-unsaturated aldehyde + 5'-phosphate DNA-termini | - |
? | |
DNA + H2O | pyrimidine dimer-specific | Tequatrovirus T4 | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? | |
DNA + H2O | moving a DNA base extrahelical opposite the target site that induces a conformational change to facilitate catalysis | Tequatrovirus T4 | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
T4 endonuclease V | - |
Tequatrovirus T4 |