Crystallization (Comment) | Organism |
---|---|
in complex with DNA containing one nucleotide A:TT loop, vapor diffusion method, using 15% (w/v) polyethylene glycol 400 and 100 mM MES, pH 6.3 | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
D43A | active site mutant | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Thermotoga maritima | the enzyme binds A:TT loops with higher affinity than undamaged DNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X2H9 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap SP column chromatography, and Superdex 75 gel filtration | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | the enzyme binds A:TT loops with higher affinity than undamaged DNA | Thermotoga maritima | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endonuclease V | - |
Thermotoga maritima |
EndoV | - |
Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
physiological function | the DNA repair enzyme recognizes and cleaves DNA at deaminated adenine lesions (hypoxanthine) In addition, the enzyme cleaves DNA containing various helical distortions such as loops, hairpins, and flaps | Thermotoga maritima |