3.1.21.7: deoxyribonuclease V
This is an abbreviated version!
For detailed information about deoxyribonuclease V, go to the full flat file.
Word Map on EC 3.1.21.7
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3.1.21.7
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nick
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deaminate
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phosphodiester
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beta-polymerase
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deoxyinosine-containing
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3\'-hydroxyl
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proofreading
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biotechnology
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analysis
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molecular biology
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drug development
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medicine
- 3.1.21.7
- nick
-
deaminate
-
phosphodiester
-
beta-polymerase
-
deoxyinosine-containing
-
3\'-hydroxyl
-
proofreading
- biotechnology
- analysis
- molecular biology
- drug development
- medicine
Reaction
endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate =
Synonyms
AGTendoV, deoxyinosine 3' endonuclease, Deoxyinosine 3'endonuclease, DNase V, EC 3.1.22.3, endo V, endodeoxyribonuclease V, endonuclease V, EndoV, Escherichia coli endodeoxyribonuclease V, nfi, PF0987, PfuEndoV, T4 endonuclease V, T4 UV endonuclease, Tb10.6k15.3890
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Inhibitors
Inhibitors on EC 3.1.21.7 - deoxyribonuclease V
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(NH4)2SO4
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50% inhibition at 25 mM, inhibition of formation of acid-soluble products
ATP
1 mM ATP almost completely inhibits hEndoV activity. hEndoV cleavage is inhibited by normal intracellular ATP concentrations. The ATP stores inside a cell do not overlay stress granules and it is suggest that hEndoV is redistributed to stress granules as a strategy to create a local environment low in ATP to permit hEndoV activity
Hg2+
Tequatrovirus T4
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50% inhibition at 0.0006 mM, inhibition of both DNA glycosylase and apurinic nicking activities, binding capability not reduced
Mn2+
at pH 8.5, a Mn2+ concentration of 2.5 mM and higher inhibits activity
additional information
Tequatrovirus T4
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an oligonucleotide duplex containing a 2'-fluorinated sugar moiety at the cyclobutane pyrimidine dimer (CPD) site inhibits the T4 Endo V reaction by stabilizing the covalent enzyme-DNA complex
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NaCl
activity is suppressed by high NaCl concentration. When adding 500 mM NaCl in the cleavage reaction, the cleavage efficiency of the enzyme is reduced to be approximately 50%. At NaCl concentrations over 800 mM, only slight endonuclease activity remains