Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.21.7: deoxyribonuclease V

This is an abbreviated version!
For detailed information about deoxyribonuclease V, go to the full flat file.

Word Map on EC 3.1.21.7

Reaction

endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate =

Synonyms

AGTendoV, deoxyinosine 3' endonuclease, Deoxyinosine 3'endonuclease, DNase V, EC 3.1.22.3, endo V, endodeoxyribonuclease V, endonuclease V, EndoV, Escherichia coli endodeoxyribonuclease V, nfi, PF0987, PfuEndoV, T4 endonuclease V, T4 UV endonuclease, Tb10.6k15.3890

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.7 deoxyribonuclease V

Inhibitors

Inhibitors on EC 3.1.21.7 - deoxyribonuclease V

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
50% inhibition at 25 mM, inhibition of formation of acid-soluble products
Ag2+
Tequatrovirus T4
-
50% inhibition at 0.01 mM
ATP
1 mM ATP almost completely inhibits hEndoV activity. hEndoV cleavage is inhibited by normal intracellular ATP concentrations. The ATP stores inside a cell do not overlay stress granules and it is suggest that hEndoV is redistributed to stress granules as a strategy to create a local environment low in ATP to permit hEndoV activity
Cu2+
Tequatrovirus T4
-
50% inhibition at 0.025 mM
Fe3+
-
strong inhibition
Hg2+
Tequatrovirus T4
-
50% inhibition at 0.0006 mM, inhibition of both DNA glycosylase and apurinic nicking activities, binding capability not reduced
KCl
above 100 mM, strong inhibition
Mn2+
at pH 8.5, a Mn2+ concentration of 2.5 mM and higher inhibits activity
NaCN
Tequatrovirus T4
-
50% inhibition at 3-5 mM
tRNA
-
half-maximal inhibition rate at 0.08 mM
additional information
Tequatrovirus T4
-
an oligonucleotide duplex containing a 2'-fluorinated sugar moiety at the cyclobutane pyrimidine dimer (CPD) site inhibits the T4 Endo V reaction by stabilizing the covalent enzyme-DNA complex
-