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3.1.21.7: deoxyribonuclease V

This is an abbreviated version!
For detailed information about deoxyribonuclease V, go to the full flat file.

Word Map on EC 3.1.21.7

Reaction

endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate =

Synonyms

AGTendoV, deoxyinosine 3' endonuclease, Deoxyinosine 3'endonuclease, DNase V, EC 3.1.22.3, endo V, endodeoxyribonuclease V, endonuclease V, EndoV, Escherichia coli endodeoxyribonuclease V, nfi, PF0987, PfuEndoV, T4 endonuclease V, T4 UV endonuclease, Tb10.6k15.3890

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.7 deoxyribonuclease V

Cloned

Cloned on EC 3.1.21.7 - deoxyribonuclease V

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in a modified version of Escherichia coli ER 2566 (ER2566DELTAnfiDELTArnhB) where the endogenous nfi and rnhB genes have been inactivated by transformation with the kanamycin-deletion cassettes derived from the JW5547-1 (nfi::kan) and JW0178-1 (rnhB::kan) strains. For crystallisation, a site specific (D52A) truncated (residues 1-250) mutant of mEndoV is fused to His/MBP in pETM41 is expressed in BL21 CodonPlus(DE3)-RIPL Escherichia coli cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIPL cells
expressed in Escherichia coli strain BL21 CodonPlus (DE3) RIL
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli GWR109
-
HEK 293T cells are transiently transfected with a vector expressing GFP-hEndoVwt
overexpression in Escherichia coli
the MBP-TbEndoV protein is overexpressed in the Escherichia coli TB1 strain