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3.1.21.7: deoxyribonuclease V

This is an abbreviated version!
For detailed information about deoxyribonuclease V, go to the full flat file.

Word Map on EC 3.1.21.7

Reaction

endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate =

Synonyms

AGTendoV, deoxyinosine 3' endonuclease, Deoxyinosine 3'endonuclease, DNase V, EC 3.1.22.3, endo V, endodeoxyribonuclease V, endonuclease V, EndoV, Escherichia coli endodeoxyribonuclease V, nfi, PF0987, PfuEndoV, T4 endonuclease V, T4 UV endonuclease, Tb10.6k15.3890

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
                3.1.21.7 deoxyribonuclease V

Crystallization

Crystallization on EC 3.1.21.7 - deoxyribonuclease V

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor-diffusion method at 25 °C, 2.4 A crystal structure
sitting-drop vapor-diffusion method at 25°C, 2.4 A crystal structure
sitting drop vapor diffusion method, using 19% (w/v) PEG 3350, 0.2 M sodium formate, 0.1 M Tris-HCl pH 7.5 or 1.4 M ammonium sulfate, 0.2 M sodium acetate, 0.1 M Tris-HCl pH 7.5
2.5 A resolution
Tequatrovirus T4
-
2.75 A resolution, complexed with a duplex DNA substrate, adenine base complementary to the 5'-side of the thymine dimer is completely flipped out of the DNA duplex
Tequatrovirus T4
-
EndoV in complex with a hypoxanthine lesion substrate and with product DNA, hanging drop vapour diffusion method, in 6-12% (w/v) MPEG 2k in 200 mM imidazole-matate buffer, pH 5.8-7.4
in complex with DNA containing one nucleotide A:TT loop, vapor diffusion method, using 15% (w/v) polyethylene glycol 400 and 100 mM MES, pH 6.3