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Literature summary for 3.1.21.7 extracted from

  • Demple, B.; Gates III, F.T.; Linn, S.
    Purification and properties of Escherichia coli endodeoxyribonuclease V (1980), Methods Enzymol., 65, 224-231.
    View publication on PubMed
Search for more literature for 3.1.21.7

General Stability

General Stability Organism
unstable to freeze-thawing, unstable for long-term storage Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ strong inhibition Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strong requirement, completely inactive if absent Escherichia coli
Mn2+ 15% of the Mg2+ enhanced activity at 2 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 glycerol density gradient sedimentation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded plasmid DNA + H2O Escherichia coli 10fold lower activity than for single-stranded circular DNA, relaxed plasmid DNA, exposure to UV light or sodium bisulfite, to pH 5.2 or osmium tetroxide renders duplex DNA 7fold more active, best substrate is uracil containing DNA from Bacillus subtilis nicked circular DNA + linearized DNA
-
 ?
single-stranded circular DNA + H2O Escherichia coli 10fold more activity than for duplex DNA linearized single-stranded DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.67
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, somewhat stable for several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded plasmid DNA + H2O 10fold lower activity than for single-stranded circular DNA, relaxed plasmid DNA, exposure to UV light or sodium bisulfite, to pH 5.2 or osmium tetroxide renders duplex DNA 7fold more active, best substrate is uracil containing DNA from Bacillus subtilis Escherichia coli nicked circular DNA + linearized DNA
-
 ?
single-stranded circular DNA + H2O 10fold more activity than for duplex DNA Escherichia coli linearized single-stranded DNA
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Escherichia coli