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Literature summary for 3.1.21.7 extracted from

  • Lee, C.; Yang, Y.; Goodman, S.; Yu, Y.; Lin, S.; Kao, J.; Tsai, K.; Fang, W.
    Endonuclease V-mediated deoxyinosine excision repair in vitro (2010), DNA Repair, 9, 1073-1079.
    View publication on PubMed
Search for more literature for 3.1.21.7

Activating Compound

Activating Compound Comment Organism Structure
ATP required Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information the deoxyinosine-containing heteroduplex is added to a purified system consisting of soluble endonuclease V fusion protein, DNA polymerase I, and DNA ligase, along with the four deoxynucleoside triphosphates. The three proteins alone are sufficient to process the dI lesion efficiently, and the 3'-exonuclease activity of DNA polymerase I is sufficient to remove the dI lesion in this minimum reconstituted assay, overview. Reconstitution of endonuclease V-mediated repair in vitro Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 several strains, gene nfi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows the repair reaction in vitro on M13mp18 derived heteroduplexes containing a site-specific deoxyinosine. Unpaired dI/G mismatch resides within the recognition site for XhoI restriction endonucleases, permitting evaluation of repair occurring on deoxyinosine-containing DNA strand Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
endonuclease V
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 endonuclease V incision assay Escherichia coli
7.9
-
 reconstituted endonuclease V-mediated excision repair assay at Escherichia coli

General Information

General Information Comment Organism
additional information the deoxyinosine-containing heteroduplex is added to a purified system consisting of soluble endonuclease V fusion protein, DNA polymerase I, and DNA ligase, along with the four deoxynucleoside triphosphates. The three proteins alone are sufficient to process the dI lesion efficiently, and the 3'-exonuclease activity of DNA polymerase I is sufficient to remove the dI lesion in this minimum reconstituted assay, overview Escherichia coli