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2.4.1.5: dextransucrase

This is an abbreviated version!
For detailed information about dextransucrase, go to the full flat file.

Word Map on EC 2.4.1.5

Reaction

sucrose
+
[(1->6)-alpha-D-glucosyl]n
=
D-fructose
 +
[(1->6)-alpha-D-glucosyl]n+1

Synonyms

B-512F dextransucrase, B-512FMC dextransucrase, Cab3, CEP, DexT, dextran-sucrase, DS, DSase, DSR, Dsr S protein, DSR-F, DSR-S, DSRB742, DSRBCB4, DSRC39-2, DsrE563, DsrP, DSRS, DSRWC, DsrX, FT045B dextransucrase, glucansucrase, glucosyltransferase, glucosyltransferase, sucrose-1,6-alpha-glucan, glycosyltransferase R, Gtf, Gtf-DSM, GTFR, More, SGE, sucrose 6-glucosyltransferase, sucrose:1, 6-alpha-D-glucan 6-alpha-glucosyltransferase, sucrose:1,6-alpha-D-glucan-6-alpha-D-glucosyltransferase, Wc392-rDSR, WcCab3-DSR

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.5 dextransucrase

Purification

Purification on EC 2.4.1.5 - dextransucrase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms: I and II
2 forms: I and N, enzyme I is gradually converted into enzyme N upon ageing, conversion is stimulated in the presence of NaCl
-
2 forms: MW 177000 and MW 158000
-
25fold purification by PEG-400 and PEG-1500 fractionation, followed by gel filtration
-
dextransucrase II
-
dextransucrase WcCab3-DSR 11fold by PEG fractionation
extracellular enzyme from kimchi by anion exchange chromatography
-
multiple forms: I, II and III
-
mutant enzyme P473S/P856S
native enzyme 37fold by PEG400 fractionation and gel filtration
native enzyme 61fold from strain NRRL B-640 by repeated polyethylene glycol fractionation and gel filtration
-
native enzyme by PEG 1500 fractionation
-
native enzyme from cell-free extracts by gel filtration
-
native enzyme from strain nRRL-B 640 by polyethylene glycol fractionation and gel filtration, with 23-40fold purification by 10% w/v PEG 1500 and 35-60fold of total purification, method optimization, overview
-
native enzyme partially by ammonium sulfate fractionation and dialysis, recombinant N-terminally His6-tagged DsrX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
native enzyme partially from strain PCSIR-4 2.5fold by ethanol
-
native extracellular enzyme 69fold by PEG1500 fractionation and gel filtration
JX679020
native extracellular enzyme by a single-step fractionation using polyethylene glycols of different molecular mass, 31fold purification by PEG 400 and 45fold purification by PEG 1500
-
native extracellular enzyme isozyme DS-III 8.55fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography
native extracellular isozyme DS-I 6.02fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography
native extracellular isozyme DS-II 5.95fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography
purified using a methodology which combines the immobilization of the enzyme in the produced biopolymer dextran, followed by a concentration step by ultrafiltration, using a membrane with a pore size of 300 kDa and subsequent hydrolysis of dextran by action of a dextranase and finally enzyme purification by anion exchange chromatography. The obtained enzyme has a purification factor of 118 and a yield of 26% from the initial extract
-
recombinant enzyme
-
recombinant enzyme 11.4fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and nickel affinity chromatography
-
recombinant enzyme 5.1fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
recombinant extracellular enzyme 4fold from Lactococcus lactis by PEG fractionation
recombinant His-tagged DSRC39-2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatogaphy
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography. Various methods such as precipitation by ammonium sulphate, ethanol, or polyethylene glycol, phase partitioning, ultrafiltration and chromatography are used to purify the enzyme, detailed overview. Purification of dextransucrase is rendered difficult by the presence of viscous dextran in the medium
-
recombinant His-tagged enzyme from Lactococcus lactis by nickel affinity chromatography
recombinant His-tagged glucansucrase 2.4fold from Escherichia coli by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes about 6fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 AI by nickel affinity chromatography
recombinant thioredoxin-His-tagged wild-type and truncated mutant C-terminal domain GBD-7 about 6fold by nickel affinity chromatography
-
recombinant wild-type and DRN1-DRN4 mutant enzymes from Escherichia coli by nickel affinity chromatography
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by anion exchange and hydrophobic interaction chromatography
recombinant wild-type DSRBCB4 and mutant fusion enzyme DXSR, partially from Escherichia coli by anion exchange chromatography and ultrafiltration
wild-type and mutant extracellular enzymes 11.35fold and 10.27fold, respectively, by PEG 4000 fractionation
-
wild-type native DsrP and DsrE from strains IBT-PQ and NRRL B-1299
-