Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 2 mM, 99% inhibition | Leuconostoc mesenteroides | |
Fe3+ | 2 mM, 97% inhibition | Leuconostoc mesenteroides | |
sucrose | substrate inhibition at concentrations over 584 mM sucrose | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
584 | - |
sucrose | pH 5, 30°C | Leuconostoc mesenteroides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Leuconostoc mesenteroides | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 2 mM, 27% increase in activity | Leuconostoc mesenteroides | |
Mg2+ | 2 mM, 37% increase in activity | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170100 | - |
calculation from sequence | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Leuconostoc mesenteroides IBUN 91.2.98 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified using a methodology which combines the immobilization of the enzyme in the produced biopolymer dextran, followed by a concentration step by ultrafiltration, using a membrane with a pore size of 300 kDa and subsequent hydrolysis of dextran by action of a dextranase and finally enzyme purification by anion exchange chromatography. The obtained enzyme has a purification factor of 118 and a yield of 26% from the initial extract | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
335.1 | - |
pH 5, 30°C | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | the produced dextran has a molecular size of 800-1000 kDa | Leuconostoc mesenteroides | D-fructose + dextran | - |
? | |
sucrose | the produced dextran has a molecular size of 800-1000 kDa | Leuconostoc mesenteroides IBUN 91.2.98 | D-fructose + dextran | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Leuconostoc mesenteroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
3 | - |
about 25% loss of activity after 20 min, about 45% loss of activity after 45 min, about 55% loss of activity after 80 min | Leuconostoc mesenteroides |
10 | - |
about 35% loss of activity after 20 min, about 35% loss of activity after 45 min, about 40% loss of activity after 80 min | Leuconostoc mesenteroides |
57 | - |
80 min, stable | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Leuconostoc mesenteroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | pH 4.0: 49% of maximal activity, pH 7.0: 25% of maximal activity | Leuconostoc mesenteroides |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leuconostoc mesenteroides | calculation from sequence | - |
4.2 |