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Information on EC 2.4.1.5 - dextransucrase
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2.4.1.5 dextransucraseIUBMB Comments
The glucansucrases transfer a D-glucosyl residue from sucrose to a glucan chain. They are classified based on the linkage by which they attach the transferred residue. In some cases, in which the enzyme forms more than one linkage type, classification relies on the relative proportion of the linkages that are generated. This enzyme extends the glucan chain by an alpha(1->6) linkage.
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Word Map
- 2.4.1.5
- streptococcus
- mutans
-
glucosylation
- glucans
- leuconostoc
-
dental
- mesenteroides
- caries
- udp-glucose
- oligosaccharide
-
cariogenic
- sobrinus
- glucoside
-
water-insoluble
- difficile
- plaque
- maltose
- glycosyltransferases
-
serotype
- saliva
- tooth
- sanguis
- gordonii
- fructosyltransferase
- udp-glc
-
monoglucosylated
- dextranase
- tcda
- glucansucrase
- pellicle
- salivarius
- gtases
-
galactosyltransferase
-
saliva-coated
- calnexin
- viscosus
-
lipid-linked
- industry
- food industry
- aureobasidium
- oralis
- pharmacology
-
pseudomembranous
- medicine
- 3-o-glucoside
- levansucrase
- biotechnology
-
alpha-1,6
- weissella
- man9glcnac2
-
transglucosylation
- synthesis
- glc3man9glcnac2
- nutrition
- isomaltose
- sordellii
-
holotoxins
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
glucosyltransferase, dextransucrase, b-512f dextransucrase, dsase, dsr-s, b-512fmc dextransucrase, dsrbcb4, dsrwc, gtf-dsm, dsrb742, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
B-512F dextransucrase
B-512FMC dextransucrase
Cab3
CEP
-
-
-
-
DexT
dextran-sucrase
-
-
-
-
DS
-
-
-
-
DSase
DSR
Dsr S protein
DSR-F
DSR-S
DSRB742
DSRBCB4
DSRC39-2
DsrE563
DSRS
DsrX
FT045B dextransucrase
glucansucrase
glucosyltransferase
glucosyltransferase, sucrose-1,6-alpha-glucan
-
-
-
-
Gtf
Gtf-DSM
SGE
-
-
-
-
sucrose 6-glucosyltransferase
-
-
-
-
sucrose:1, 6-alpha-D-glucan 6-alpha-glucosyltransferase
Wc392-rDSR
additional information
DSRS
-
-
-
-
additional information
-
the enzyme belongs to the glycoside hydrolase family 70
additional information
-
the enzyme belongs to the glycoside hydrolase family 70
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
-
-
-
sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1
involvement of the three conserved amino acids (Asp551, Glu589, and Asp662) in the organic reaction enzyme mechanism for the biosynthesis of dextran at the active site of dextransucrase by the insertion mechanism addition of D-glucose to the reducing-end of a growing dextran chain
-
sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1
catalytic reaction mechanism for dextran elongation, overview
-
sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1
involvement of the three conserved amino acids (Asp551, Glu589, and Asp662) in the organic reaction enzyme mechanism for the biosynthesis of dextran at the active site of dextransucrase by the insertion mechanism addition of D-glucose to the reducing-end of a growing dextran chain
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
sucrose:(1->6)-alpha-D-glucan 6-alpha-D-glucosyltransferase
The glucansucrases transfer a D-glucosyl residue from sucrose to a glucan chain. They are classified based on the linkage by which they attach the transferred residue. In some cases, in which the enzyme forms more than one linkage type, classification relies on the relative proportion of the linkages that are generated. This enzyme extends the glucan chain by an alpha(1->6) linkage.
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CAS REGISTRY NUMBER
COMMENTARY
9032-14-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
luteolin + sucrose
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
sucrose + 1,5-anhydro-D-fructose
alpha-D-glucopyranosyl-(1,6)-1,5-anhydro-D-fructose + alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-O-1,5-anhydro-D-fructose + alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-O-1,5-anhydro-D-fructose
sucrose + 2-chloroethanol
2-chloroethyl alpha-D-glucopyranoside + D-fructose
-
-
-
-
?
sucrose + 4-chlorobutanol
4-chlorobutyl alpha-D-glucopyranoside + D-fructose
-
-
-
-
?
sucrose + 6-chlorohexanol
6-chlorohexyl alpha-D-glucopyranoside + D-fructose
-
-
-
-
?
sucrose + alpha-butylglucopyranoside
alpha-D-glucopyranosyl-(1,6)-O-butyl-alpha-D-glucopyranoside + alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-O-butyl-alpha-D-glucopyranoside
-
-
-
-
?
sucrose + butan-1-ol
butyl alpha-D-glucopyranoside + D-fructose
-
-
-
-
?
sucrose + gentobiose
?
-
glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview
-
-
?
sucrose + maltose
?
-
glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview
-
-
?
sucrose + methanol
methyl alpha-D-glucopyranoside + D-fructose
-
-
-
-
?
sucrose + N-(tert-butoxycarbonyl)-L-serine methyl ester
N-tert-butoxycarbonyl-3-O-alpha-D-glucopyranosyl-L-serine methyl ester + D-fructose
-
-
-
-
?
sucrose + N-tert-butoxycarbonyl-D-serine methyl ester
N-tert-butoxycarbonyl-3-O-alpha-D-glycopyranosyl-D-serine methyl ester + D-fructose
-
-
-
-
?
sucrose + propan-1-ol
propyl alpha-D-glucopyranoside + D-fructose
-
-
-
-
?
sucrose + salicin
?
-
glucose transfer from donor sucrose to acceptors releasing D-fructose, acceptor specificity of wild-type and mutant enzymes, overview
-
-
?
sucrose + stevioside
13-O-beta-sophorosyl-19-O-beta-isomaltosyl-steviol + 13-O-(beta-(1->6) glucosyl)-beta-glucosylsophorosyl-19-O-beta-isomaltosyl-steviol + 13-O-beta-sophorosyl-19-O-beta-isomaltotriosyl-steviol
sucrose + tert-butanol
D-fructose + tert-butyl alpha-D-glucoside
-
-
-
-
?
1'-beta-D-fructofuranosyl alpha-acarbose
D-fructose + acarbose
-
-
-
-
?
1'-beta-D-fructofuranosyl alpha-acarbose
D-fructose + acarbose
-
-
-
-
?
luteolin + sucrose
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
-
44% conversion
luteolin-3'-O-alpha-D-glucopyranoside is the major product
-
?
luteolin + sucrose
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
-
44% conversion
luteolin-3'-O-alpha-D-glucopyranoside is the major product
-
?
luteolin + sucrose
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
-
44% conversion
luteolin-3'-O-alpha-D-glucopyranoside is the major product
-
?
sucrose
D-fructose + ?
a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies
-
-
?
sucrose
D-fructose + ?
a dextransucrase efficient in synthesizing oligosaccharides is designed. The truncation mutant DSR-S1-DELTAA (residues 1-3087 bp) by deleting the 1494 bp fragment of the C-terminal.The mutant enzyme (MW: 110 kDa) loses activity, when sucrose is used as only substrate. After adding an acceptor, DSR-S1-DELTAA is fully activated but with heavily impaired polysaccharide synthesis ability. The enzyme produces a large amount of oligosaccharides. DSR-S1-DELTAA shows transglycosylation for synthesizing more oligosaccharides of lower degree of polymerization (DP) with different acceptors, and it also improves the selection range of dextransucrase acceptor response to acceptors. The enzyme can be applied in glycodiversifcation studies
-
-
?
sucrose
D-fructose + ?
dextransucrase capable of producing a dextran polysaccharide with four types of linkages, including 69% (alpha1->6), 24% (alpha1->3), 6% (alpha1->4), and 1% (alpha1->2)
-
-
?
sucrose
D-fructose + ?
dextransucrase capable of producing a dextran polysaccharide with four types of linkages, including 69% (alpha1->6), 24% (alpha1->3), 6% (alpha1->4), and 1% (alpha1->2)
-
-
?
sucrose
D-fructose + dextran
-
free and immobilized enzyme produces 5.7 mg/ml and 2.6 mg/ml of dextran in 2 l bench scale fermenter under optimum reaction conditions
-
-
?
sucrose
D-fructose + dextran
dextran that is produced by wild-type enzyme has 95% alpha(1->6) linkages in the main chains and 5% alpha(1->3) branch linkage. the dextran synthesized by mutant P473S/P856S shows almost no obvious change with comparison of the wild-type enzyme
-
-
?
sucrose
D-fructose + dextran
-
the produced dextran has a molecular size of 800-1000 kDa
-
-
?
sucrose
D-fructose + dextran
dextran that is produced by wild-type enzyme has 95% alpha(1->6) linkages in the main chains and 5% alpha(1->3) branch linkage. the dextran synthesized by mutant P473S/P856S shows almost no obvious change with comparison of the wild-type enzyme
-
-
?
sucrose
D-fructose + dextran
Leuconostoc mesenteroides IBUN 91.2.98
-
the produced dextran has a molecular size of 800-1000 kDa
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
high amounts of enzyme catalyze the hydrolysis of the D-glucose residues from the ends of the dextran chains, giving a decrease in the amount of dextran
-
r
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
native enzyme produces mainly 6-linked glucopyranosylresidues, while Escherichia coli recombinant enzyme produces a glucan consisting of 70% 6-linked glucopyranosyl residues and 15% 3,6-glucopyranosyl residues. Mutant enzyme T350K and S455K produce a glucan with 85% 6-linked glucopyranosyl residues. The mutant T350K/S455K produces adhesive, water-insoluble glucan with 77% 6-linked glucopyranosyl residues, 8% 3,6-linked glucopyranosyl residues and 4% 2,6-linked glucopyranosyl residues
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
with increasing concentrations of sucrose, from 0.1 to 4.0 M, the amount of high-molecular weight dextran decreases with a concomitant increase in low-molecular weight dextran. At 0.1 M sucrose, pH 5.5, and 28°C, 99.8% of the dextran had a MW of more than 1000000 Da and at 4.0 M sucrose, 69.9% have a MW below 100000 Da and 30.1% have a MW of more than 1000000 Da, giving a bimodal distribution. The degree of branching increased from 5% for 0.1 M sucrose to 16.6% for 4.0 M sucrose. The temperature has very little effect on the size of the dextran, which is above 1000000 Da, but it has a significant effect on the degree of branching, which is 4.8% at 4 °C and increases to 14.7% at 45°C. Both the molecular weight and the degree of branching are not significantly affected by different pH values between 4.5 and 6.0
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
dextransucrase preferentially produces an isomaltooligosaccharide series, whose concentration is always low because of the high ability of these products to be elongated and form high molecular weight dextran. In dextransucrase, the A repeats define anchoring zones for the growing chains, favoring their elongation. Based on these results, a semi-processive mechanism involving only one active site and an elongation by the non-reducing end is proposed
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
high amounts of enzyme catalyze the hydrolysis of the D-glucose residues from the ends of the dextran chains, giving a decrease in the amount of dextran
-
r
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
with increasing concentrations of sucrose, from 0.1 to 4.0 M, the amount of high-molecular weight dextran decreases with a concomitant increase in low-molecular weight dextran. At 0.1 M sucrose, pH 5.5, and 28°C, 99.8% of the dextran had a MW of more than 1000000 Da and at 4.0 M sucrose, 69.9% have a MW below 100000 Da and 30.1% have a MW of more than 1000000 Da, giving a bimodal distribution. The degree of branching increased from 5% for 0.1 M sucrose to 16.6% for 4.0 M sucrose. The temperature has very little effect on the size of the dextran, which is above 1000000 Da, but it has a significant effect on the degree of branching, which is 4.8% at 4 °C and increases to 14.7% at 45°C. Both the molecular weight and the degree of branching are not significantly affected by different pH values between 4.5 and 6.0
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n
D-fructose + (1,6-alpha-D-glucosyl)n+1
-
native enzyme produces mainly 6-linked glucopyranosylresidues, while Escherichia coli recombinant enzyme produces a glucan consisting of 70% 6-linked glucopyranosyl residues and 15% 3,6-glucopyranosyl residues. Mutant enzyme T350K and S455K produce a glucan with 85% 6-linked glucopyranosyl residues. The mutant T350K/S455K produces adhesive, water-insoluble glucan with 77% 6-linked glucopyranosyl residues, 8% 3,6-linked glucopyranosyl residues and 4% 2,6-linked glucopyranosyl residues
-
-
?
sucrose + (1,6-alpha-D-glucosyl)n