Literature summary for 2.4.1.5 extracted from

Shukla, S.; Shi, Q.; Maina, N.H.; Juvonen, M.; Maijatenkanen, M.; Goyal, A.
Weissella confusa Cab3 dextransucrase: properties and in vitro synthesis of dextran and glucooligosaccharides (2014), Carbohydr. Polym., 101, 554-564.

Search for more literature for 2.4.1.5

Application

Application	Comment	Organism
synthesis	purified Weissella confusa Cab3 dextransucrase (WcCab3-DSR) is used for in vitro synthesis of dextran and glucooligosaccharides	Weissella confusa

General Stability

General Stability	Organism
0.1% glycerol and 1.0% Tween 80 enhance enzyme stability, enzyme half-life at 30°C increases from 10 h to 74 h and 59 h, respectively, stabilization by 0.01 mg/ml PEG-8000	Weissella confusa
dextran T-500 slightly stabilizes	Weissella confusa
glutaraldehyde slightly destabilizes	Weissella confusa

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	98% inhibition at 5 mM	Weissella confusa
Mn2+	42% inhibition at 10 mM	Weissella confusa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates by 20% at 1 mM	Weissella confusa
Co2+	activates by 21% at 1 mM	Weissella confusa
additional information	poor effects by Zn2+, Mg2+, and Ni2+ at 1-2 mM	Weissella confusa

Organism

Organism	UniProt	Comment	Textmining
Weissella confusa	A0A0F6TP16	-	-

Purification (Commentary)

Purification (Comment)	Organism
dextransucrase WcCab3-DSR 11fold by PEG fractionation	Weissella confusa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10.9	-	purified enzyme, pH 5.4, 35°C	Weissella confusa

Storage Stability

Storage Stability	Organism
-20°C, purified enzyme, half-life: 37.9 days	Weissella confusa
-4°C, purified enzyme, half-life: 69.4 days	Weissella confusa
30°C, purified enzyme, half-life: 10.33 h	Weissella confusa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	Cab3 dextransucrase WcCab3-DSR is used for in vitro synthesis of dextran and glucooligosaccharides, product analysis by NMR and and mass spectrometry. Glucooligosaccarides are produced through the acceptor reaction with maltose	Weissella confusa	?	-	?
sucrose + maltose	-	Weissella confusa	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	glucooligosaccarides are produced through the acceptor reaction with maltose, a homologous series of isomaltooligosac-charides with reducing end maltose units	?
sucrose + [(1->6)-alpha-D-glucosyl]n	substrate is dextran	Weissella confusa	D-fructose + [(1->6)-alpha-D-glucosyl]n+1	the produced dextran confirmes the presence of main chain alpha-(1->6) linkages with only 3.0% of alpha-(1->3) branching, of which some are elongated	?

Synonyms

Synonyms	Comment	Organism
Cab3	-	Weissella confusa
WcCab3-DSR	-	Weissella confusa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Weissella confusa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	glycerol and Tween 80 enhance enzyme stability, enzyme half-life at 30°C increases from 10 h to 74 h and 59 h, respectively	Weissella confusa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.4	-	-	Weissella confusa

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Release 2023.1 (January 2023)