Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.1.5: dextransucrase

This is an abbreviated version!
For detailed information about dextransucrase, go to the full flat file.

Word Map on EC 2.4.1.5

Reaction

sucrose
+
[(1->6)-alpha-D-glucosyl]n
=
D-fructose
 +
[(1->6)-alpha-D-glucosyl]n+1

Synonyms

B-512F dextransucrase, B-512FMC dextransucrase, Cab3, CEP, DexT, dextran-sucrase, DS, DSase, DSR, Dsr S protein, DSR-F, DSR-S, DSRB742, DSRBCB4, DSRC39-2, DsrE563, DsrP, DSRS, DSRWC, DsrX, FT045B dextransucrase, glucansucrase, glucosyltransferase, glucosyltransferase, sucrose-1,6-alpha-glucan, glycosyltransferase R, Gtf, Gtf-DSM, GTFR, More, SGE, sucrose 6-glucosyltransferase, sucrose:1, 6-alpha-D-glucan 6-alpha-glucosyltransferase, sucrose:1,6-alpha-D-glucan-6-alpha-D-glucosyltransferase, Wc392-rDSR, WcCab3-DSR

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.5 dextransucrase

General Stability

General Stability on EC 2.4.1.5 - dextransucrase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
0.1% glycerol and 1.0% Tween 80 enhance enzyme stability, enzyme half-life at 30°C increases from 10 h to 74 h and 59 h, respectively, stabilization by 0.01 mg/ml PEG-8000
alginate entrapment enhances the thermal stability of dextransucrase for up to 3 days in orange juice at 30°C
-
Ca2+ is required for stability
-
Ca2+ significantly stabilizes the soluble enzyme
-
Ca2+ stabilizes the enzyme
-
dextran T-500 slightly stabilizes
dextran T10 stabilizes
-
dextransucrase cross-linked enzyme aggregates maintain 40% of their activity after 10 cycles
-
enzyme deactivation follows first order rate kinetics
-
enzyme immobilized on calcium-alginate (3% w/v) retains activity for 11 batch reactions without a decrease in activity
-
glutaraldehyde does not support the stability, rather it inhibits the enzyme activity, also glycerol, PEG 8000, and dextran (500 kDa) do not stabilize the enzyme
-
glutaraldehyde slightly destabilizes
glycerol and Tween 80 stabilize the enzyme 2fold and 12fold, respectively, overview
JX679020
glycerol enhances the stability at 25-40°C, enzyme stability at 35°C with glycerol is higher than at 25°C without glycerol
immobilization on calcium alginate as entrapment matrix increases heat stability of the enzyme
-
maximal stabilization of soluble enzyme by 5 mM Ca2+
-
native enzyme loses 90% of its inital activity in only 2 h at pH 5.4, 30°C, immobilization on Eupergit C 250L significantly stabilizes dextransucrase. Despite a fast partial inactivation in the first 2 h, the enzyme immobilized on Eupergit C 250L maintains more than 40% of the initial activity over the following 2 days, the enzyme immobilized on Eupergit C maintains about 15% of the initial activity after 1 day
-
PEG8000 stabilizes moderately with 39% activity remaining after 12 h
JX679020
stability is significantly increased by 33% glycerol and 0.1% bovine serum albumin
-
stability of crude dextransucrase from Leuconostoc citreum strain B-742 in synthetic and in cashew apple juice culture broth, evaluation, overview
-
the best conditions for the enzyme are 50% v/v dimethyl sulfoxide and immobilization in alginate beads
-
the enzyme can be stabilized by high-molecular-weight dextran, polyethyleneglycol or nonionic detergents such as Tween 80
-
the immobilization on calcium-alginate (3% w/v) improves the stability of the enzyme
-
Tween 80 stabilizes the purified enzyme, maximally at 30°C
-