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2.4.1.5: dextransucrase

This is an abbreviated version!
For detailed information about dextransucrase, go to the full flat file.

Word Map on EC 2.4.1.5

Reaction

sucrose
+
[(1->6)-alpha-D-glucosyl]n
=
D-fructose
 +
[(1->6)-alpha-D-glucosyl]n+1

Synonyms

B-512F dextransucrase, B-512FMC dextransucrase, Cab3, CEP, DexT, dextran-sucrase, DS, DSase, DSR, Dsr S protein, DSR-F, DSR-S, DSRB742, DSRBCB4, DSRC39-2, DsrE563, DsrP, DSRS, DSRWC, DsrX, FT045B dextransucrase, glucansucrase, glucosyltransferase, glucosyltransferase, sucrose-1,6-alpha-glucan, glycosyltransferase R, Gtf, Gtf-DSM, GTFR, More, SGE, sucrose 6-glucosyltransferase, sucrose:1, 6-alpha-D-glucan 6-alpha-glucosyltransferase, sucrose:1,6-alpha-D-glucan-6-alpha-D-glucosyltransferase, Wc392-rDSR, WcCab3-DSR

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.5 dextransucrase

Temperature Stability

Temperature Stability on EC 2.4.1.5 - dextransucrase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
about 35% loss of activity after 20 min, about 35% loss of activity after 45 min, about 40% loss of activity after 80 min
10 - 30
-
purified enzyme, stable at, rapid loss of activity above. Loss of 65% activity within 20 h at 30°C without added stabilizer, in presence of Tween 80 the enzyme loses only 8% activity. Loss of 92% activity at 30°C within 4 days
10 - 50
purified isozyme 2, thermostable at
10 - 60
15 - 37
stable, rapid decrease in activity above 37°C
20 - 30
purified recombinant enzyme, in 20 mM sodium acetate, pH 5.4, 30 min, stable, rapid loss of activity above 30°C
28
partially purified recombinant fusion enzyme DXSR, stable
3
-
about 25% loss of activity after 20 min, about 45% loss of activity after 45 min, about 55% loss of activity after 80 min
35 - 40
enzyme stability decreases sharply when the temperature is raised from 35°C to 40°C, half-life at 35°C is 26.3 h and at 40°C 7.4 h without glycerol, and 37.5 h and 11.2 h with glycerol, respectively
35.7
T50-value of wild-type enzyme
37
-
T1/2: 6 h (soluble enzyme), T1/2: 9 h (enzyme immobilized on calcium-alginate)
37.5
T50-value of mutant P473S/P856S
4 - 60
-
the native DsrX shows higher stability than the recombinant DsrX at the temperature range from 4°C to 60°C for 2 h, native enzyme shows 84% remaining activity at 40°C after 2 h, while recombinant enzyme shows only 3.1%
57
-
80 min, stable
70
-
half-life: 4 min (soluble enzyme), half-life: 42 min (enzyme immobilized on calcium-alginate)
additional information