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Literature summary for 2.4.1.5 extracted from

  • Chen, Z.; Tian, Y.; Zhang, W.; Guang, C.; Meng, X.; Mu, W.
    Novel dextransucrase Gtf-DSM, highly similar in sequence to reuteransucrase GtfO, displays unique product specificity (2019), J. Agric. Food Chem., 67, 12806-12815 .
    View publication on PubMed
Search for more literature for 2.4.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Limosilactobacillus ingluviei

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 1 mM, slight inhibition Limosilactobacillus ingluviei
Mn2+ 1 mM, slight inhibition Limosilactobacillus ingluviei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, enhances the transferase activity by 116% Limosilactobacillus ingluviei
Co2+ 1 mM, enhances the transferase activity by 25% Limosilactobacillus ingluviei
Cu2+ 1 mM, enhances the transferase activity by 25% Limosilactobacillus ingluviei
Mg2+ 1 mM, enhances the transferase activity by 10% Limosilactobacillus ingluviei
Ni2+ 1 mM, enhances the transferase activity by 40% Limosilactobacillus ingluviei
Zn2+ 1 mM, enhances the transferase activity by 30% Limosilactobacillus ingluviei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117000
-
 SDS-PAGE Limosilactobacillus ingluviei
117400
-
 calculated from sequence Limosilactobacillus ingluviei

Organism

Organism UniProt Comment Textmining
Limosilactobacillus ingluviei A0A0R2GUC5
-
-
Limosilactobacillus ingluviei DSM 14792 A0A0R2GUC5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Limosilactobacillus ingluviei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose dextransucrase capable of producing a dextran polysaccharide with four types of linkages, including 69% (alpha1->6), 24% (alpha1->3), 6% (alpha1->4), and 1% (alpha1->2) Limosilactobacillus ingluviei D-fructose + ?
-
 ?
sucrose dextransucrase capable of producing a dextran polysaccharide with four types of linkages, including 69% (alpha1->6), 24% (alpha1->3), 6% (alpha1->4), and 1% (alpha1->2) Limosilactobacillus ingluviei DSM 14792 D-fructose + ?
-
 ?

Synonyms

Synonyms Comment Organism
Gtf-DSM
-
 Limosilactobacillus ingluviei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 transglycosylation activity Limosilactobacillus ingluviei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 40 30°C: about 85% of maximal activity, 40°C: about 85% of maximal activity Limosilactobacillus ingluviei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Limosilactobacillus ingluviei

pH Range

pH Minimum pH Maximum Comment Organism
2.5 7 pH 2.5: about 75% of maximal activity, pH 7.0: about 70% of maximal activity Limosilactobacillus ingluviei