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Literature summary for 2.4.1.5 extracted from

  • Kajala, I.; Shi, Q.; Nyyssoelae, A.; Maina, N.H.; Hou, Y.; Katina, K.; Tenkanen, M.; Juvonen, R.
    Cloning and characterization of a Weissella confusa dextransucrase and its application in high fibre baking (2015), PLoS ONE, 10, e0116418.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.5

Application

Application Comment Organism
food industry the enzyme from Weissella confusa strain VTT E-90392 is useful in dextran production during sour dough production. The hydrocolloidal properties of dextran can facilitate a more substantial use of wheat bran and counter the negative effects of bran on bread quality, optimization of enzymatic dextran production in wheat bran Weissella confusa

Cloned(Commentary)

Cloned (Comment) Organism
gene dsr, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Lactococcus lactis Weissella confusa

General Stability

General Stability Organism
glycerol enhances the stability at 25-40°C, enzyme stability at 35°C with glycerol is higher than at 25°C without glycerol Weissella confusa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Weissella confusa
17.4
-
 sucrose pH 5.4, 35°C, recombinant enzyme Weissella confusa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Weissella confusa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 x * 160000, recombinant His-tagged enzyme, SDS-PAGE Weissella confusa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n Weissella confusa substrate is dextran D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n Weissella confusa VTT E-90392 substrate is dextran D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Weissella confusa X4QKX0
-
-
Weissella confusa VTT E-90392 X4QKX0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Lactococcus lactis by nickel affinity chromatography Weissella confusa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2
-
 recombinant His-tagged enzme, by the 14C-sucrose radioisotope assay, pH 5.4, 35°C Weissella confusa
18.9
-
 recombinant His-tagged enzme, by and Nelson-Somogyi assay, pH 5.4, 35°C Weissella confusa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information optimization of enzymatic dextran production in wheat bran, overview Weissella confusa ?
-
 ?
additional information optimization of enzymatic dextran production in wheat bran, overview Weissella confusa VTT E-90392 ?
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1 product determination by NMR spectroscopy ?
sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Weissella confusa VTT E-90392 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Weissella confusa VTT E-90392 D-fructose + [(1->6)-alpha-D-glucosyl]n+1 product determination by NMR spectroscopy ?

Subunits

Subunits Comment Organism
? x * 160000, recombinant His-tagged enzyme, SDS-PAGE Weissella confusa
More enzyme tertiary structure modeling Weissella confusa

Synonyms

Synonyms Comment Organism
Wc392-rDSR
-
 Weissella confusa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 recombinant enzyme Weissella confusa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 over 65% activity within this range, purified recombinant enzyme Weissella confusa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 40 enzyme stability decreases sharply when the temperature is raised from 35°C to 40°C, half-life at 35°C is 26.3 h and at 40°C 7.4 h without glycerol, and 37.5 h and 11.2 h with glycerol, respectively Weissella confusa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.2
-
 sucrose pH 5.4, 35°C, recombinant enzyme Weissella confusa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 recombinant enzyme Weissella confusa

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 over 60% activity within this range, purified recombinant enzyme Weissella confusa

General Information

General Information Comment Organism
evolution sequence comparison with sequence alignment of Weissella confusa LBAE C39-2 dextransucrase and Lactobacillus reuteri 180, GTF180 Weissella confusa
additional information structure and size of in vitro dextran product is similar to dextran produced in vivo. Enzymatic production of dextran into wheat bran, overview Weissella confusa