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Literature summary for 2.4.1.5 extracted from
- Entrapment of purified novel dextransucrase obtained from newly isolated Acetobacter tropicalis and its comparative study of kinetic parameters with free enzyme (2019), Biocatal. Biotransform., 37, 349-360 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the increased reusability, higher pH range and storage stability of immobilized enzyme as compared with free enzyme can increase the sustainability and applicability of the enzyme in industries | Acetobacter tropicalis |
General Stability
| General Stability | Organism |
|---|---|
| enzyme immobilized on calcium-alginate (3% w/v) retains activity for 11 batch reactions without a decrease in activity | Acetobacter tropicalis |
| the immobilization on calcium-alginate (3% w/v) improves the stability of the enzyme | Acetobacter tropicalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | drastically decreases the specific activity in soluble enzyme and immobilized enzyme form | Acetobacter tropicalis |  |
| Fe2+ | drastically decreases the specific activity in soluble enzyme and immobilized enzyme form | Acetobacter tropicalis | |
| Pb2+ | drastically decreases the specific activity in soluble enzyme and immobilized enzyme form | Acetobacter tropicalis | |
| Zn2+ | drastically decreases the specific activity in soluble enzyme and immobilized enzyme form | Acetobacter tropicalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.5 | - |
sucrose | pH 5.5, 37°C, soluble enzyme | Acetobacter tropicalis | |
| 29 | - |
sucrose | pH 5.5, 37°C, enzyme immobilized on calcium-alginate | Acetobacter tropicalis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | both the free and immobilized dextransucrase work best with 0.2 mM CaCl2 and exhibit 6062 and 5830 U/mg specific activity, respectively | Acetobacter tropicalis | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| urea | stable in presence of urea | Acetobacter tropicalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacter tropicalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetobacter tropicalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5830 | - |
pH 5.5, 37°C, enzyme immobilized on calcium-alginate beads. Substrate: sucrose | Acetobacter tropicalis |
| 6062 | - |
pH 5.5, 37°C, soluble enzyme. Substrate: sucrose | Acetobacter tropicalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| the free as well as immobilized enzyme are most stable at 4°C for more than 1 month | Acetobacter tropicalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose | free and immobilized enzyme produces 5.7 mg/ml and 2.6 mg/ml of dextran in 2 l bench scale fermenter under optimum reaction conditions | Acetobacter tropicalis | D-fructose + dextran | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
the enzyme works best at pH 5.5 and 37°C for 30 min in both the free as well as immobilized (on calcium-alginate) state | Acetobacter tropicalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
T1/2: 8 h (soluble enzyme), T1/2: 10 h (enzyme immobilized on calcium-alginate) | Acetobacter tropicalis |
| 37 | - |
T1/2: 6 h (soluble enzyme), T1/2: 9 h (enzyme immobilized on calcium-alginate) | Acetobacter tropicalis |
| 40 | - |
soluble enzyme is very unstable, t1/2: 9 h (enzyme immobilized on calcium-alginate) | Acetobacter tropicalis |
| 50 | - |
half-life: 4.68 min (soluble enzyme), half-life: 385 min (enzyme immobilized on calcium-alginate) | Acetobacter tropicalis |
| 60 | - |
half-life: 4.68 min (soluble enzyme), half-life: 295 min (enzyme immobilized on calcium-alginate) | Acetobacter tropicalis |
| 70 | - |
half-life: 4 min (soluble enzyme), half-life: 42 min (enzyme immobilized on calcium-alginate) | Acetobacter tropicalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
the enzyme works best at pH 5.5 and 37°C for 30 min in both the free as well as immobilized (on calcium-alginate) state | Acetobacter tropicalis |
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