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Literature summary for 2.4.1.5 extracted from
- Purification of the dimeric form of dextransucrase from Leuconostoc mesenteroides strains NRRL B-146 and B-1375 (1985), Agric. Biol. Chem., 49, 1385-1391.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dextrans | addition of 0.5% exogenous dextran activates B-1416 enzyme 4.35fold, B-1375 enzyme is activated 2.76fold | Leuconostoc mesenteroides |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | 133000 | disc gel electrophoresis | Leuconostoc mesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | - |
B-1375 | - |
| Leuconostoc mesenteroides | - |
NRRL B-1416 | - |
| Leuconostoc mesenteroides B-1375 | - |
B-1375 | - |
| Leuconostoc mesenteroides NRRL B-1416 | - |
NRRL B-1416 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| B-1375 | Leuconostoc mesenteroides |
| B-1416 | Leuconostoc mesenteroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Leuconostoc mesenteroides |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.1% bovine serum albumin, pH 5.2-8.5, stable for 24 h | Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
| sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides NRRL B-1416 | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
| sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides B-1375 | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 64000-68000, SDS-PAGE after alkaline treatment, pH 10.5 | Leuconostoc mesenteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
B-1375 enzyme with dextran | Leuconostoc mesenteroides |
| 35 | - |
B-1416 enzyme with and without dextran, B-1375 enzyme without dextran | Leuconostoc mesenteroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
B-1375 enzyme stable | Leuconostoc mesenteroides |
| 40 | - |
B-1416 enzyme is stable up to | Leuconostoc mesenteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Leuconostoc mesenteroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 8.5 | 4°C, 0.1% bovine serum albumin, stable for 24 h, rapid inactivation below pH 5.0 | Leuconostoc mesenteroides |
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