Application | Comment | Organism |
---|---|---|
food industry | the enzyme is a good food additive for improving the textures of dairy products due to dextran synthesis, e.g. solidification of sucrose-supplemented milk by the enzyme | Pediococcus pentosaceus |
General Stability | Organism |
---|---|
glycerol and Tween 80 stabilize the enzyme 2fold and 12fold, respectively, overview | Pediococcus pentosaceus |
PEG8000 stabilizes moderately with 39% activity remaining after 12 h | Pediococcus pentosaceus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 18% inhibition at 2 mM, 89% at 8 mM | Pediococcus pentosaceus | |
Ni2+ | 12% inhibition at 1 mM, 90% at 8 mM | Pediococcus pentosaceus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Pediococcus pentosaceus | |
15.4 | - |
sucrose | pH 5.4, 30°C | Pediococcus pentosaceus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pediococcus pentosaceus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates by 44% at 2 mM | Pediococcus pentosaceus | |
Co2+ | stimulates by 56% at 4 mM | Pediococcus pentosaceus | |
Mg2+ | stimulates by 14% at 2 mM | Pediococcus pentosaceus | |
Zn2+ | stimulates by 12% at 1 mM | Pediococcus pentosaceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
224000 | - |
x * 224000, SDS-PAGE | Pediococcus pentosaceus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->6)-alpha-D-glucosyl]n | Pediococcus pentosaceus | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Pediococcus pentosaceus CRAG3 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pediococcus pentosaceus | JX679020 | isolated from fermented cucumber | - |
Pediococcus pentosaceus CRAG3 | JX679020 | isolated from fermented cucumber | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 69fold by PEG1500 fractionation and gel filtration | Pediococcus pentosaceus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46 | - |
purified native enzyme, pH 5.4, 30°C | Pediococcus pentosaceus |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, most stable at, half-life is 307 days, 95% activity remains afer 21 days | Pediococcus pentosaceus |
4°C, purified enzyme, half-life is 129 days, 89% activity remains afer 21 days | Pediococcus pentosaceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with raffinose | Pediococcus pentosaceus | ? | - |
? | |
additional information | no activity with raffinose | Pediococcus pentosaceus CRAG3 | ? | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Pediococcus pentosaceus | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | best at 5% sucrose in 20 mM sodium acetate buffer | Pediococcus pentosaceus | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Pediococcus pentosaceus CRAG3 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | best at 5% sucrose in 20 mM sodium acetate buffer | Pediococcus pentosaceus CRAG3 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 224000, SDS-PAGE | Pediococcus pentosaceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pediococcus pentosaceus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, stable up to, rapid loss of activity above, 35% activity remains after 24 h at 30°C, 93% after 9 h at 30°C in presence of glycerol | Pediococcus pentosaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Pediococcus pentosaceus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | 7 | purified enzyme, stable at, 82% activity remains after 3 h at pH 5.4, 21% and 12% activity remains at pH 4.0 and pH 3.5, respectively | Pediococcus pentosaceus |