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Literature summary for 2.4.1.5 extracted from
- Purified dextransucrase from Pediococcus pentosaceus CRAG3 as food additive (2014), Indian J. Exp. Biol., 52, 1036-1044.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme is a good food additive for improving the textures of dairy products due to dextran synthesis, e.g. solidification of sucrose-supplemented milk by the enzyme | Pediococcus pentosaceus |
General Stability
| General Stability | Organism |
|---|---|
| glycerol and Tween 80 stabilize the enzyme 2fold and 12fold, respectively, overview | Pediococcus pentosaceus |
| PEG8000 stabilizes moderately with 39% activity remaining after 12 h | Pediococcus pentosaceus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 18% inhibition at 2 mM, 89% at 8 mM | Pediococcus pentosaceus |  |
| Ni2+ | 12% inhibition at 1 mM, 90% at 8 mM | Pediococcus pentosaceus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Pediococcus pentosaceus | |
| 15.4 | - |
sucrose | pH 5.4, 30°C | Pediococcus pentosaceus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pediococcus pentosaceus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates by 44% at 2 mM | Pediococcus pentosaceus | |
| Co2+ | stimulates by 56% at 4 mM | Pediococcus pentosaceus | |
| Mg2+ | stimulates by 14% at 2 mM | Pediococcus pentosaceus | |
| Zn2+ | stimulates by 12% at 1 mM | Pediococcus pentosaceus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 224000 | - |
x * 224000, SDS-PAGE | Pediococcus pentosaceus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + [(1->6)-alpha-D-glucosyl]n | Pediococcus pentosaceus | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
| sucrose + [(1->6)-alpha-D-glucosyl]n | Pediococcus pentosaceus CRAG3 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pediococcus pentosaceus | JX679020 | isolated from fermented cucumber | - |
| Pediococcus pentosaceus CRAG3 | JX679020 | isolated from fermented cucumber | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 69fold by PEG1500 fractionation and gel filtration | Pediococcus pentosaceus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 46 | - |
purified native enzyme, pH 5.4, 30°C | Pediococcus pentosaceus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme, most stable at, half-life is 307 days, 95% activity remains afer 21 days | Pediococcus pentosaceus |
| 4°C, purified enzyme, half-life is 129 days, 89% activity remains afer 21 days | Pediococcus pentosaceus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with raffinose | Pediococcus pentosaceus | ? | - |
? | |
| additional information | no activity with raffinose | Pediococcus pentosaceus CRAG3 | ? | - |
? | |
| sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Pediococcus pentosaceus | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
| sucrose + [(1->6)-alpha-D-glucosyl]n | best at 5% sucrose in 20 mM sodium acetate buffer | Pediococcus pentosaceus | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
| sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Pediococcus pentosaceus CRAG3 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
| sucrose + [(1->6)-alpha-D-glucosyl]n | best at 5% sucrose in 20 mM sodium acetate buffer | Pediococcus pentosaceus CRAG3 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 224000, SDS-PAGE | Pediococcus pentosaceus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pediococcus pentosaceus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme, stable up to, rapid loss of activity above, 35% activity remains after 24 h at 30°C, 93% after 9 h at 30°C in presence of glycerol | Pediococcus pentosaceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Pediococcus pentosaceus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 7 | purified enzyme, stable at, 82% activity remains after 3 h at pH 5.4, 21% and 12% activity remains at pH 4.0 and pH 3.5, respectively | Pediococcus pentosaceus |
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