Cloned (Comment) | Organism |
---|---|
the recombinant wild-type enzyme and mutant proteins are expressed from Escherichia coli BL21 star (DE3) | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
A670V | the mutation increases the percentage of alpha(1->3) linkages (9% at most) and decreases the percentage of alpha(1->6) linkages in the product | Leuconostoc mesenteroides |
D590A | the mutation introduces a decrease the percentage of alpha(1->3) linkage in the product | Leuconostoc mesenteroides |
E664K | the mutation introduces additional alpha(1->3) glycosidic linkages and also some additional alpha(1->2) glycosidic linkages, the mutation decreases the percentage of alpha(1->6) linkages in the product | Leuconostoc mesenteroides |
additional information | DSR-S1-DELTAV (residues 1-1425) and DSR-S2-DELTA(V) (residues 1-1279) are constructed by deleting partial YG repeats of domain V. DSR-S3-DELTA(V) (residues 1-1160), DSR-S-DELTA IV (residues 1-1124), and DSR-S-DELTA(B) (residues 1-1110) are constructed by deleting the relevant fragments from C-terminal ends. The truncation mutant DSR-S1-DELTA(A) (residues 1-1029) is constructed by deleting partial domain A while containing complete conserved Motif regions I. DSR-S2-DELTA(A) (residues 1-1022) is constructed by deleting partial domain A including conserved Motif regions I. DSRS3-DELTA(A) (residues 1-1000) is constructed by deleting more domain A fragment, allowing further investigation of the functions of C-terminal end domain. 102 amino acids of C-terminal end has no effect on dextran synthesis, but it will improve enzyme protein expression by deleting these amino acids. After further deletion, polysaccharidesynthesizing capability of dextransucrase will be inhibited. With the addition of maltose as postreceptors, truncated enzymes undergoes glycosylation reaction and transferred glucosyl from sucrose to acceptor effectively. By deleting the 417 amino acid fragment, its oligosaccharide synthesizing capability significantly increases. This is an effective way to make use of dextransucrase for prebiotic synthesis | Leuconostoc mesenteroides |
N555Y | the mutation increases the percentage of alpha(1->3) linkages (9% at most) and decreases the percentage of alpha(1->6) linkages in the product | Leuconostoc mesenteroides |
Q1029K | the mutation has no significant effect on the linkage specificity | Leuconostoc mesenteroides |
Q666R | the mutation introduces a small amount of additional alpha(1->4) glycosidic linkages, the mutation decreases the percentage of alpha(1->6) linkages in the product | Leuconostoc mesenteroides |
S663N | the mutation increases the percentage of alpha(1->3) linkages (9% at most) and decreases the percentage of alpha(1->6) linkages in the product | Leuconostoc mesenteroides |
V553A | the mutation introduces a small amount of additional alpha(1->4) glycosidic linkages, the mutation decreased the production of alpha(1->3) linkage polysaccharides | Leuconostoc mesenteroides |
V556I | the mutation introduces a small amount of additional alpha(1->4) glycosidic linkages, the mutation decreased the production of alpha(1->3) linkage polysaccharides | Leuconostoc mesenteroides |
V665A | the mutation introduces a small amount of additional alpha(1->4) glycosidic linkages, the mutation decreases the percentage of alpha(1->6) linkages in the product | Leuconostoc mesenteroides |
W591G | the mutation introduces a decrease the percentage of alpha(1->3) linkage in the product | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics analysis of continuously truncated enzymes with sucrose and maltose as the substrate | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | Q2I2N5 | - |
- |
Leuconostoc mesenteroides 0326 | Q2I2N5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + maltose | - |
Leuconostoc mesenteroides | D-fructose + ? | - |
? | |
sucrose + maltose | - |
Leuconostoc mesenteroides 0326 | D-fructose + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Leuconostoc mesenteroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics analysis of continuously truncated enzymes with sucrose and maltose as the substrate | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Leuconostoc mesenteroides |