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Literature summary for 2.4.1.5 extracted from
- Mutational analysis and characterization of dextran synthesizing enzyme from wild and mutant strain of Leuconostoc mesenteroides (2013), Carbohydr. Polym., 91, 209-216.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | citrate buffer at 0.3 M results in highest activity of wild-type and mutant strains, overview | Leuconostoc mesenteroides |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of diverse mutant enzymes using UV irradiation random mutagensis, mutant screeening, overview. Mutant KIBGE IB-22M20 exhibits 6.75fold increased dextransucrase activity compared to the wild-type enzyme | Leuconostoc mesenteroides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 77.09 | - |
sucrose | partially purified wild-type enzyme, pH 4.5, 30°C | Leuconostoc mesenteroides |  |
| 101.7 | - |
sucrose | partially purified mutant enzyme, pH 5.0, 35°C | Leuconostoc mesenteroides | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Leuconostoc mesenteroides | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 221000 | - |
x * 221000, partially purified enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | - |
isolated from fermented cabbage | - |
| Leuconostoc mesenteroides KIBGE IB-22 | - |
isolated from fermented cabbage | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type and mutant extracellular enzymes 11.35fold and 10.27fold, respectively, by PEG 4000 fractionation | Leuconostoc mesenteroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
dextransucrae activity in different strains, i.e. KIBGE IB-06, KIBGE IB-07, KIBGE IB-08, KIBGE IB-09, KIBGE IB-10, KIBGE IB-11, KIBGE IB-12, KIBGE IB-13, KIBGE IB-19, and KIBGE IB-22, all isolated from different sources, phylogenetic tree, overview. Strain KIBGE IB-22 exhibits the highest enzyme activity | Leuconostoc mesenteroides |
| 31.3 | - |
partially purified wild-type enzyme, pH 4.5, 30°C | Leuconostoc mesenteroides |
| 173.2 | - |
partially purified mutant enzyme, pH 5.0, 35°C | Leuconostoc mesenteroides |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 221000, partially purified enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
wild-type enzyme | Leuconostoc mesenteroides |
| 35 | - |
mutant enzyme | Leuconostoc mesenteroides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 45 | mutant enzyme, activity range | Leuconostoc mesenteroides |
| 15 | 40 | wild-type enzyme, activity range | Leuconostoc mesenteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
wild-type enzyme | Leuconostoc mesenteroides |
| 5 | - |
mutant enzyme | Leuconostoc mesenteroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
no enzyme activity at neutral pH by the wild-type strain | Leuconostoc mesenteroides |
| 4 | 7.5 | mutant enzyme, maximal activity at pH 5.0, 25% of maximal activity at pH 4.0, and 7.5% of maximal activity at pH 7.5 | Leuconostoc mesenteroides |
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