Information on EC 3.1.1.47 - 1-alkyl-2-acetylglycerophosphocholine esterase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
3.1.1.47
-
RECOMMENDED NAME
GeneOntology No.
1-alkyl-2-acetylglycerophosphocholine esterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of carboxylic ester
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ether lipid metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine acetohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
76901-00-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-didecanoyl-sn-glycerol + H2O
?
show the reaction diagram
1,2-dioctanoyl-sn-glycerol + H2O
?
show the reaction diagram
1,2-dioctanoylethyleneglycol + H2O
?
show the reaction diagram
1-acetyl-2-hexadecyl-sn-glycero-3-phosphocholine + H2O
2-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
1-acyl-2-linoleoyl-glycero-phosphocholine + H2O
2-linoleoyl-glycero-phosphocholine + acylic acid
show the reaction diagram
-
-
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
1-decanoyl-rac-glycerol + H2O
?
show the reaction diagram
-
-
-
?
1-decanoyl-rac-glycerol + H2O
decanoate + glycerol
show the reaction diagram
-
-
-
?
1-hexadecyl-2-acetyl-sn-glycerol-3-phosphocholine + H2O
1-hexadecyl-sn-glycerol-3-phosphocholine + acetate
show the reaction diagram
1-hexadecyl-2-butyryl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-glycero-3-phosphocholine + butanoate
show the reaction diagram
-
-
-
?
1-hexadecyl-2-glutaryl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-glycero-3-phosphocholine + glutarate
show the reaction diagram
1-hexadecyl-2-propionyl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-glycero-3-phosphocholine + propionate
show the reaction diagram
-
-
-
?
1-hexadecyl-2-succinyl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-glycero-3-phosphocholine + succinate
show the reaction diagram
1-myristoyl-2-(4-nitrophenyl succinyl)phosphatidylcholine + H2O
1-myristoyl-phosphatidylcholine + 4-nitrophenyl succinate
show the reaction diagram
-
artificial substrate
4-nitrophenyl succinate spontaneously degrades and liberates 4-nitrophenol
?
1-myristoyl-2-(4-nitrophenylsuccinyl)-3-phosphatidylcholine + H2O
1-myristoyl-3-phosphatidylcholine + 4-nitrophenylsuccinate
show the reaction diagram
-
-
-
-
?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
regulation of platelet-activating factor
-
-
?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid + H2O
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate
show the reaction diagram
-
preferred substrate for the alpha1/alpha1 homodimer and the alpha1/alpha2 heterodimer
-
?
1-O-alkyl-2-butyryl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + butanoate
show the reaction diagram
-
-
-
?
1-O-alkyl-2-propionyl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + propanoate
show the reaction diagram
-
-
-
?
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-hexadecyl-sn-glycerol-3-phosphocholine + acetate
show the reaction diagram
1-O-hexadecyl-2-N-methylcarbamoyl-sn-glycero-3-phospho(N,N,N-trimethyl)hexanolamine
?
show the reaction diagram
-
-
-
-
?
1-O-hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
-
-
-
?
1-octanoyl-rac-glycerol + H2O
?
show the reaction diagram
-
-
-
?
1-octanoyl-rac-glycerol + H2O
octanoate + glycerol
show the reaction diagram
-
-
-
?
1-oleoyl-2-acetyl-sn-glycerol + H2O
1-oleoyl-sn-glycerol + acetate
show the reaction diagram
1-palmitoyl-2(5-oxovaleroyl)-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + 5-oxopentanoate
show the reaction diagram
-
plasma isozyme
-
?
1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine + H2O
5-oxopentanoate + 1-palmitoyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + arachidonate
show the reaction diagram
-
plasma isozyme
-
?
1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + glutarate
show the reaction diagram
-
plasma isozyme
-
?
1-palmitoyl-2-oxopentanoyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + 2-oxopentanoate
show the reaction diagram
-
-
-
?
1-palmitoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
substrate activity assay
-
-
?
1-palmitoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
substrate activity assay
-
-
?
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine
show the reaction diagram
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-acetyl-1-O-alkyl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
i.e. platelet-activation factor, hydrolysis of sn-2 ester bond of platelet-activating factor PAF and related inflammatory phospholipids, no activity with the erythrocyte isozyme
-
?
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoethanolamine + H2O
1-O-alkyl-sn-glycero-3-phosphoethanolamine + acetate
show the reaction diagram
-
-
-
?
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate
show the reaction diagram
2-acetyl-1-octadecyl-sn-glycero-3-phosphocholine + H2O
1-octadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-acetyl-3-octadecyl-sn-glycero-1-phosphocholine + H2O
3-octadecyl-sn-glycero-1-phosphocholine + acetate
show the reaction diagram
2-butanoyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-butanoyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
transacetylase activity, 40% of the activity with PAF
-
?
2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-propionyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-propionyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
transacetylase activity, 73% of the activity with PAF
-
?
2-thio platelet-activating factor + H2O
?
show the reaction diagram
-
substrate activity assay
-
-
?
2-thio-[platelet activating factor] + H2O
acetate + deacetylated 2-thio-[platelet activating factor]
show the reaction diagram
-
-
-
-
?
2-valeroyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-valeroyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
transacetylase activity, 17% of the activity with PAF
-
?
3-O-hexadecyl-2-acetyl-sn-glycero-1-phosphocholine + H2O
3-O-hexadecyl-sn-glycero-1-phosphocholine + acetate
show the reaction diagram
4-(phenylthio)-N-(4-phenyl-2-oxobutyl)azetidin-2-one + H2O
thiophenol + 3-oxopropanoic acid + 1-amino-4-phenyl-2-butanone
show the reaction diagram
-
-
-
?
acetylated platelet-activation factor + H2O
?
show the reaction diagram
-
-
-
?
azelaoyl phosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
butyl acetate + H2O
butanol + acetate
show the reaction diagram
dibutyryl-phosphocholine + H2O
?
show the reaction diagram
didecanoyl-phosphocholine + H2O
?
show the reaction diagram
dioctanoyl-phosphocholine + H2O
?
show the reaction diagram
F2-isoprostane phospholipid + H2O
F2-isoprostane + phospholipid
show the reaction diagram
-
plays key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo, involved in oxidative stress response
-
-
?
lipoteichoic acid + H2O
?
show the reaction diagram
-
PAF-AH removes one acyl chain at the sn-2 position of pneumococcal lipoteichoic acid and produces a monoacyl-LTA that is inactive against mouse cells
-
-
?
methyl butyrate + H2O
methanol + butyrate
show the reaction diagram
octyl acetate + H2O
octanol + acetate
show the reaction diagram
oxidized phosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
?
oxidized phospholipids + H2O
?
show the reaction diagram
phospholipid + H2O
?
show the reaction diagram
-
degradation of phospholipids produced by oxidative stress
-
-
?
phospholipids + H2O
?
show the reaction diagram
platelet activating factor + H2O
acetate + deacetylated platelet activating factor
show the reaction diagram
platelet-activating factor + H2O
?
show the reaction diagram
platelet-activating factor + H2O
lyso-platelet-activating factor + acetate
show the reaction diagram
-
-
-
-
?
triacetylglycerol + H2O
diacetylglycerol + acetate
show the reaction diagram
tributyrylglycerol + H2O
dibutyrylglycerol + butyrate
show the reaction diagram
trioctanoylglycerol + H2O
dioctanoylglycerol + octanoate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
regulation of platelet-activating factor
-
-
?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
1-O-hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine + H2O
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
-
-
-
?
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
1-alkyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecanoyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
transacetylation
-
?
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-sn-glycero-3-phosphocholine
1-hexadecyl-sn-glycero-3-phosphocholine + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
the transacetylase activity may acetylate extracellular lyso-PAF into biologically active PAF
-
?
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate
show the reaction diagram
2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
1-hexadecyl-sn-glycero-3-phosphocholine + acetate
show the reaction diagram
-
i.e. platelet-activation factor, substrate and enzyme play important roles in inflammation processes
-
?
3-O-hexadecyl-2-acetyl-sn-glycero-1-phosphocholine + H2O
3-O-hexadecyl-sn-glycero-1-phosphocholine + acetate
show the reaction diagram
F2-isoprostane phospholipid + H2O
F2-isoprostane + phospholipid
show the reaction diagram
-
plays key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo, involved in oxidative stress response
-
-
?
oxidized phospholipids + H2O
?
show the reaction diagram
phospholipid + H2O
?
show the reaction diagram
-
degradation of phospholipids produced by oxidative stress
-
-
?
phospholipids + H2O
?
show the reaction diagram
-
hydrolyzes sn-2-ester bonds of fragmented or oxidized phospholipids, enzyme deficiency results in an increased risk of stroke, coronary artery disease, atherosclerotic occlusive disease, and abdominal aortic aneurysm
-
-
?
platelet activating factor + H2O
acetate + deacetylated platelet activating factor
show the reaction diagram
platelet-activating factor + H2O
?
show the reaction diagram
platelet-activating factor + H2O
lyso-platelet-activating factor + acetate
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-Alkyl-2,3-diacetyl-sn-glycerol
-
slight
1-Hexadecyl-2-acetyl-sn-glycero-3-phosphoethanolamine
-
-
1-O-alkyl-2-acetyl-sn-glycero-3-phosphodimethylethanolamine
-
-
1-O-alkyl-2-acetyl-sn-glycero-3-phosphoethanolamine
-
-
1-O-alkyl-2-acetyl-sn-glycero-3-phosphomonomethylethanolamine
-
-
1-O-alkyl-2-propionyl-sn-glycero-3-phosphocholine
-
-
1-O-hexadecanoyl-sn-glycero-3-phosphocholine
-
competitive inhibition of acetylhydrolase activity
1-O-hexadecyl-2-acetyl-rac-glycerol
-
-
1-O-hexadecyl-2-N-methylcarbamoyl-sn-glycero-3-phospho(N,N,N-trimethyl)hexanolamine
-
-
1-O-hexadecyl-2-N-methylcarbamoyl-sn-glycero-3-phosphocholine
-
-
1-O-hexadecyl-2-O-methyl-sn-glycero-3-phosphocholine
-
-
1-O-hexadecyl-2-thioacetyl-sn-glycero-3-phosphocholine
1-oleyl-2-acetyl-sn-glycerol
-
-
2-acetyl-1-acyl-glycero-sn-glycero-3-phosphocholine
-
-
2-N-methylcarbamyl platelet-activating factor
3-Hexadecyl-2-acetyl-sn-glycero-1-phosphocholine
-
-
4-(2-aminoethyl)benzenesulfonyl fluoride
-
i.e. pefabloc, inhibits both acetylhydrolase and transacetylase activities completely at 0.05 mM for the native enzyme, and at 1 mM for the recombinant enzyme
4-(phenylthio)-N-(4-phenyl-2-oxobutyl)azetidin-2-one
-
-
4-bromophenacyl bromide
-
-
5,5'-dithiobis(2-nitrobenzoate)
5,5'-dithiobis-2-nitrobenzoic acid
-
atorvastatin
-
reduces LDL-associated enzyme activity by reduction of LDL amount, does not reduce enzyme secretion by macrophages
benzodioxaphosphorin 2-oxides
BN 50739
-
antagonists of lipopolysaccharides, inhibition of induction of plasma enzyme expression
Ca2+
-
18% inhibition at 10 mM
chlorpyrifos oxon
corticoids
-
regulatory function on the enzyme form from HL-60 cells
-
croton oil
-
regulatory function on the enzyme form from plasma
-
CV 3988
-
-
deoxycholate
-
-
diazoxon
diethyldicarbonate
-
-
diisopropyl fluorophosphates
-
diisopropylfluorophosphate
dithioerythritol
-
-
DTNB
-
69% inhibition at 2 mM
Estrogens
-
regulatory function on the enzyme form from plasma
-
fenofibrate
-
decreases total plasma and LDL_associated enzyme activity but does not influence HDL-associated enzyme activity
IFN-gamma
-
regulatory function on the enzyme form from monocyte-derived macrophages
-
interleukin-1alpha
-
regulatory function on the enzyme form from decidual macrophages
-
Interleukin-1beta
-
regulatory function on the enzyme form from decidual macrophages
-
interleukin-8
-
regulatory function on the enzyme form from decidual and monocyte-derived macrophages
-
iodoacetamide
lipopolysaccharides
lyso-2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine
-
22% inhibition at 0.05 mM
-
lyso-platelet-activating factor
-
regulatory function on the enzyme form from hepatocytes
methoxy arachidonyl fluorophosphonate
-
type 1 PAF acetylhydrolase is irreversibly inhibited
methyl arachidonyl fluorophosphonate
n-alkyl methylphosphonofluoridates
N-tosyl-L-lysine chloromethyl ketone
-
-
N-tosyl-L-phenylalanyl chloromethyl ketone
-
-
p-bromophenacyl bromide
p-bromophenacylbromide
p-nitroguanidinobenzoate
-
-
pefabloc
-
-
pepstatin A
-
-
phenylmethylsulfonyl fluoride
phosphatidylcholine
-
from egg, long chain diacetyl type, slight
phosphocholine
-
25% inhibition at 0.05 mM
platelet activating factor
-
competitive
SB-677116
-
-
-
SB222657
-
-
Sulfonyl fluorides
tribufos
tumor growth factor-beta
-
regulatory function on the enzyme form from HL-60 cells
-
tumor necrosis factor-alpha
-
regulatory function on the enzyme form from decidual macrophages
-
WEB 2170
-
antagonists of lipopolysaccharides, inhibition of induction of plasma enzyme expression
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
corticoids
deoxycholate
-
activation
dithiothreitol
dyslipidemia
-
induces an increase in total plasma enzyme activity and alters the enzyme distribution between proatherogenic apoB- and antiatherogenic apo L-containing lipoproteins by increasing the enzyme activity associated with apoB-containing lipoproteins, atorvastin and fenofibrate can restore the normal level by reduction of LDL amount
-
GM-CSF
-
regulatory function on the enzyme form from monocyte-derived macrophages
-
interleukin-beta
-
regulatory function on the enzyme form from plasma, liver and spleen
-
lipopolysaccharides
M-CSF
-
regulatory function on the enzyme form from monocyte-derived macrophages
-
platelet-activating factor
progestin
tumor necrosis factor-alpha
-
regulatory function on the enzyme form from plasma, liver and spleen
-
turpentine
-
regulatory function on the enzyme form from plasma
-
zymosan
-
regulatory function on the enzyme form from plasma
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011
1,2-didecanoyl-sn-glycerol
-
plasma isozyme, pH 7.5, 22C
0.005
1,2-dioctanoyl-sn-glycerol
0.086
1-decanoyl-rac-glycerol
-
plasma isozyme, pH 7.5, 22C
0.0031
1-hexadecyl-2-acetyl-sn-glycerol-3-phosphocholine
-
-
0.0052 - 0.392
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine
1.4
1-octanoyl-rac-glycerol
-
plasma isozyme, pH 7.5, 22C
0.0193 - 0.0431
1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine
0.0049 - 0.0137
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
0.005
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
0.006
2-acetyl-1-oleoyl-sn-glycerol
-
plasma isozyme, pH 7.5, 22C
0.082 - 0.206
acetylated platelet-activation factor
63
butyl acetate
-
plasma isozyme, pH 7.5, 22C
4.7
dibutanoyl-phosphocholine
-
plasma isozyme, pH 7.5, 22C
0.005
didecanoyl-phosphocholine
-
below, plasma isozyme, pH 7.5, 22C
0.047
dioctanoyl-phosphocholine
-
plasma isozyme, pH 7.5, 22C
0.0000045 - 0.000057
F2-isoprostane-phosphocholine
143
methyl butyrate
-
plasma isozyme, pH 7.5, 22C
0.055
octyl acetate
-
plasma isozyme, pH 7.5, 22C
2.1
platelet activating factor
-
native enzyme, pH 7.2, 37C
131
triacetylglycerol
-
plasma isozyme, pH 7.5, 22C
0.136
tributyryl-glycerol
-
plasma isozyme, pH 7.5, 22C
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1 - 8.1
1-acetyl-2-hexadecyl-sn-glycero-3-phosphocholine
0.34 - 24.6
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine
0.68 - 6.08
2-acetyl-1-octadecyl-sn-glycero-3-phosphocholine
0.25 - 3
2-acetyl-1-oleoyl-sn-glycerol
0.28 - 6.08
octyl acetate
1.7 - 37
triacetylglycerol
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29 - 0.47
platelet activating factor
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000001
-
acetylhydrolase activity mg tissue, mammary artery, plus Pefablok
0.0000003
-
acetylhydrolase activity mg tissue, aorta, plus Pefablok
0.0000005
-
mutant S257A, substrate azelaoyl phosphatidylcholine
0.0000014
-
acetylhydrolase activity mg tissue, mammary artery
0.0000028
-
acetylhydrolase activity mg tissue, aorta
0.000008
-
cytosolic fraction, nonpregnant uterus
0.00001
-
wild type, substrate azelaoyl phosphatidylcholine
0.000012
-
PAF-AH isozyme Ib alpha2 subunit, transacetylase activity
0.000073
-
cytosolic fraction, pregnant uterine myometrium
0.000221
-
cytosolic fraction, myoma
0.00028
-
PAF-AH isozyme Ib alpha1 subunit, acetylhydrolase activity
0.00123
-
-
0.00236
-
PAF-AH isozyme Ib alpha2 subunit, acetylhydrolase activity
0.01572
-
-
0.02
-
partially purified enzyme
0.022
-
acetylhydrolase activity
0.038
-
-
0.0408
-
PAF-AH isozyme II, transacetylase activity
0.0445
-
plasma enzyme
0.105
-
PAF-AH isozyme II, acetylhydrolase activity
0.114
-
plasma PAF-AH, transacetylase activity
0.131
-
plasma PAF-AH, acetylhydrolase activity
0.176
-
purified enzyme, 37C, pH 7.0
0.47
-
supernatant fraction
0.55
-
alveolar type II cells and 1000 x g fraction
0.77
-
macrophages
0.805
-
purified from erythrocytes
0.85
-
100000 x g fraction
1.32
-
60000 x g fraction
1.45
-
intracellular enzyme form Ib from brain
3.7
-
bronchoalveolar lavage
4.2
-
purifed enzyme using 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine as substrate
4.5
-
Ca2+-dependent PLA2, cell-free hemolymph of Rhodnius prolixus feeding with blood plus dexamethasone
6.7
-
Ca2+-dependent PLA2, control, cell-free hemolymph of Rhodnius prolixus after regular blood feeding
7.2
-
cytosolic isoform II from liver
9.46
-
plasma enzyme
10.7
-
Ca2+-dependent PLA2, cell-free hemolymph of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes
12
-
Ca2+-independent PLA2, cell-free hemolymph of Rhodnius prolixus feeding with blood plus dexamethasone
14
-
Ca2+-independent PLA2, control, cell-free hemolymph of Rhodnius prolixus after regular blood feeding
17
-
total PLA2, cell-free hemolymph of Rhodnius prolixus feeding with blood plus dexamethasone
21
-
total PLA2, control, cell-free hemolymph of Rhodnius prolixus after regular blood feeding
24
-
PAF-AH, control, cell-free hemolymph of Rhodnius prolixus after regular blood feeding
27.1
-
plasma
32
-
Ca2+-independent PLA2, cell-free hemolymph of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes
34
-
PAF-AH, cell-free hemolymph of Rhodnius prolixus feeding with blood plus dexamethasone
43
-
total PLA2, cell-free hemolymph of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes
119
-
Ca2+-independent PLA2, hemocyte of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes
140
-
Ca2+-independent PLA2, hemocyte of Rhodnius prolixus feeding with blood plus dexamethasone
186
-
Ca2+-independent PLA2, control, hemocyte of Rhodnius prolixus after regular blood feeding
203
-
PAF-AH, cell-free hemolymph of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes
381
-
Ca2+-dependent PLA2, hemocyte of Rhodnius prolixus feeding with blood plus dexamethasone
427
-
Ca2+-dependent PLA2, hemocyte of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes
529
-
total PLA2, hemocyte of Rhodnius prolixus feeding with blood plus dexamethasone
570
-
total PLA2, hemocyte of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes
702
-
Ca2+-dependent PLA2, control, hemocyte of Rhodnius prolixus after regular blood feeding
915
-
total PLA2, control, hemocyte of Rhodnius prolixus after regular blood feeding
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
-
-
7.6
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
-
broad range, inactivation below pH 4.0
5 - 7.4
-
inactivation below pH 5.0, active to mild alkaline range
additional information
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at
25
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
atherogenic aorta
Manually annotated by BRENDA team
-
nonatherogenic mammary artery
Manually annotated by BRENDA team
-
melanoma cell line, implanted in syngenic C57BI/6J mice which then show reduced vascularization and growth
Manually annotated by BRENDA team
-
platelets contain the plasma type as well as the intracellular type II PAF-AH. Platelets contain high levels of the mRNA of plasma PAF-AH, whereas only a small quantity of the type II PAF-AH mRNA is detected. Platelets secrete the plasma type of platelet-activating factor acetylhydrolase primarily associated with microparticles
Manually annotated by BRENDA team
-
isozyme II, of children with meningitis
Manually annotated by BRENDA team
Q29460 and P68401
the greatest PAF-AH activity is detected on the day of parturition (day 0), after which its activity dramatically decreases and by day 5, the enzyme activity is less than 7% of the level at parturition
Manually annotated by BRENDA team
-
monocyte-derived cells show very low enzyme expression
Manually annotated by BRENDA team
-
isozyme in endometrium is of the plasma enzyme type, enzyme activity decreases on day 20 in pregnant cows compared to cyclic cows on the same day, which show no changes in enzyme activity level
Manually annotated by BRENDA team
-
secretion of plasma isozyme
Manually annotated by BRENDA team
-
epidermal, isozyme II, lower amount compared to sebaceous gland
Manually annotated by BRENDA team
-
Kaposi's sarcoma cell line, implanted into SCID mice which then show reduced vascularization and growth
Manually annotated by BRENDA team
-
MM6 cell
Manually annotated by BRENDA team
-
enzyme activity increases during differentiation, only monocyte-derived dendritic cells show highly reduced enzyme expression
Manually annotated by BRENDA team
-
at least 2 isozymes I and II, increased amount of isozyme I beta-subunit
Manually annotated by BRENDA team
-
at least 2 isozymes I and II
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
clonal derivative of PC12 cell line
Manually annotated by BRENDA team
-
robust expression of PLA2G7 mRNA
Manually annotated by BRENDA team
-
PAF-AH activity in the salivary gland homogenates is lower than in the saliva
Manually annotated by BRENDA team
-
PAF-AH activity in the salivary gland homogenates is lower than in the saliva
Manually annotated by BRENDA team
-
isozyme II, high amount
Manually annotated by BRENDA team
-
the activity is significantly and negatively correlated with sperm motility. Platelet-activating factor acetylhydrolase serves as a decapacitation factor
Manually annotated by BRENDA team
-
isozyme II
Manually annotated by BRENDA team
-
isozyme I activity is lower in pregnant uterus compared to the nonpregnant one
Manually annotated by BRENDA team
-
robust expression of PLA2G7 mRNA
Manually annotated by BRENDA team
additional information