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Literature summary for 3.1.1.47 extracted from
- Intracellular erythrocyte PAF acetylhydrolase 1 inactivates aspirin in blood (2011), J. Biol. Chem., 286, 34820-34829.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of PAFAH1B2 or PAFAH1B3 subunits in HEK-293T cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methoxy arachidonyl fluorophosphonate | type 1 PAF acetylhydrolase is irreversibly inhibited | Homo sapiens |  |
| NaF | - |
Homo sapiens | |
| platelet activating factor | competitive | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
| 2.1 | - |
platelet activating factor | native enzyme, pH 7.2, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Homo sapiens | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 30000 | - |
1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 45000 | - |
1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
| 70000 | - |
native enzyme, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the type 1 PAF acetylhydrolase is one of three mammalian family members of the group 7 phospholipase A2 family, a family that is unique in that all members require a short sn-2 ester such as the acetyl ester of PAF | ? | - |
? | |
| platelet activating factor + H2O | Homo sapiens | erythrocyte and plasma PAF acetylhydrolases | acetate + deacetylated platelet activating factor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 1400fold from erythrocytes by two different steps of anion exchange chromatography, followed by gel filtration, recombinant PAFAH1B2 or PAFAH1B3 subunits from HEK-293T cells | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | erythrocyte aspirin hydrolysis varies among individuals | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the type 1 PAF acetylhydrolase is one of three mammalian family members of the group 7 phospholipase A2 family, a family that is unique in that all members require a short sn-2 ester such as the acetyl ester of PAF | Homo sapiens | ? | - |
? | |
| additional information | recombinant PAFAH1B2, but not its family member plasma PAF acetylhydrolase, hydrolyzes acetylsalicylic acid to acetate and salicylate, activity of EC 3.1.1.55 | Homo sapiens | ? | - |
? | |
| platelet activating factor + H2O | erythrocyte and plasma PAF acetylhydrolases | Homo sapiens | acetate + deacetylated platelet activating factor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | type I PAF acetylhydrolase is a trimer of a noncatalytic 45 kDa protein subunit associated with two catalytic subunits, PAFAH1B3 and PAFAH1B2 as homo- or heterodimers | Homo sapiens |
| trimer | 1 * 45000, noncatalytic subunit, + 1 * 29000, subunit alpha1 or PAFAH1B3, + 1 * 30000, subunit alpha2 or PAFAH1B2, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aspirin hydrolase | - |
Homo sapiens |
| More | type I PAF acetylhydrolase is a structurally unrelated member of this small family of group VII phospholipases A2 | Homo sapiens |
| PAF acetylhydrolase | - |
Homo sapiens |
| PAF acetylhydrolase I | - |
Homo sapiens |
| PAFAH | - |
Homo sapiens |
| plasma PAF acetylhydrolase | - |
Homo sapiens |
| type I PAF acetylhydrolase | - |
Homo sapiens |
| type I platelet-activating factor acetylhydrolase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
platelet activating factor | recombinant catalytic subunit PAFAH1B2, pH 7.2, 37°C | Homo sapiens | |
| 0.47 | - |
platelet activating factor | native enzyme, pH 7.2, 37°C | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | type I platelet-activating factor acetylhydrolase is a phospholipase A2 with selectivity for acetyl residues of PAF, and acts as aspirin acetylhydrolase. The intracellular erythrocyte PAF acetylhydrolase 1 inactivates aspirin in blood, and is the major aspirin hydrolase of human blood | Homo sapiens |
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Release 2023.1 (January 2023)
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