Information on EC 2.7.7.6 - DNA-directed RNA polymerase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.7.6
-
RECOMMENDED NAME
GeneOntology No.
DNA-directed RNA polymerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
nucleoside triphosphate + RNAn = diphosphate + RNAn+1
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Purine metabolism
-
-
Pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
nucleoside-triphosphate:RNA nucleotidyltransferase (DNA-directed)
Catalyses DNA-template-directed extension of the 3'- end of an RNA strand by one nucleotide at a time. Can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. See also EC 2.7.7.19 (polynucleotide adenylyltransferase) and EC 2.7.7.48 (RNA-directed RNA polymerase).
CAS REGISTRY NUMBER
COMMENTARY hide
9014-24-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PCC 7120
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Autographa californica M nucleopolyhedrovirus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
DSM 1731
-
-
Manually annotated by BRENDA team
RNA polymerase I, II and III
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene k1ep
UniProt
Manually annotated by BRENDA team
several strains, gene rpoA
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain MHOM/IL/81/Friedlin
-
-
Manually annotated by BRENDA team
Leishmania major MHOM/IL/81/Friedlin
strain MHOM/IL/81/Friedlin
-
-
Manually annotated by BRENDA team
strain UR6
-
-
Manually annotated by BRENDA team
strain UR6
-
-
Manually annotated by BRENDA team
the nucleotide sequence of a part, 8334 bp, of the EcoRI fish lymphocystis disease virus DNA fragment B, between the EcoRI site and 259 nucleotides downstream from the second PstI site has been deposited in GenBank accession number: L34213
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
encoded on the linear mitochondrial plasmid
-
-
Manually annotated by BRENDA team
strain mc2155
-
-
Manually annotated by BRENDA team
strain mc2155
-
-
Manually annotated by BRENDA team
two RNA polymerase paralogues rpoB(S) and rpoB(R)
-
-
Manually annotated by BRENDA team
scorpionfly, collected in June in the village of Toksovo, Leningrad region, Russia
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
PpY101
-
-
Manually annotated by BRENDA team
strain WCS358
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain yBC-10
-
-
Manually annotated by BRENDA team
single phage-type RNA polymerase gene rpoT
UniProt
Manually annotated by BRENDA team
strain DSS12, a deep-sea piezophilic bacterium, genes rpoE2 and rpoE3
-
-
Manually annotated by BRENDA team
strain DSS12, a deep-sea piezophilic bacterium, genes rpoE2 and rpoE3
-
-
Manually annotated by BRENDA team
Q980R2: subunit A', P58192: subunit A'', Q980R1: subunit B, P95989: subunit D, Q980A3: subunit E', Q9UXD9: subunit F, Q980L5: subunit G, Q980Q9: subunit H, Q97ZJ9: subunit K, Q980K0: subunit L, Q980Z8: subunit N, Q97ZX7: subunit P, Q980B8: subunit 13
Q980R2 and P58192 and Q980R1 and P95989 and Q980A3 and Q9UXD9 and Q980L5 and Q980Q9 and Q97ZJ9 and Q980K0 and Q980Z8 and Q97ZX7 and Q980B8
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain WR
-
-
Manually annotated by BRENDA team
strain WR
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
UniProt
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
UniProt
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-C-methyl-ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
misincorporation frequency of approximately 1 in 7800 2'-C-methyl-ATP
-
-
?
2'-deoxy-ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
misincorporation
-
-
?
3'-deoxy-ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
misincorporation frequency of approximately 1 in 5 3'-dATP
-
-
?
ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
CTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
d(Ap4T) + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
d(TP4C) + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
d(Tp4G) + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
d(Tp4T) + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
DNA + 5-[[(2-aminoethyl)amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[(2-methylpropyl)amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[(2-pyridinylmethyl)amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[(4-pyridinylmethyl)amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[benzylamino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[[2-(1H-imidazol-4-yl)ethyl]amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[[2-(1H-indol-3-yl)ethyl]amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
dTTP + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
GTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
nucleoside triphosphate + A10G2A2C2C
?
show the reaction diagram
-
oligonucleotide extension
-
-
?
nucleoside triphosphate + A9G3A2C2C
?
show the reaction diagram
-
oligonucleotide extension
-
-
?
nucleoside triphosphate + G2CAC2C
?
show the reaction diagram
-
oligonucleotide extension
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
show the reaction diagram
nucleoside triphosphate + T10G2T2C2C
?
show the reaction diagram
-
oligonucleotide extension
-
-
?
rNTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
CTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
GTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe-S cluster
-
with 4Fe-4S cluster-binding motif
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
can replace Mg2+ in activation, 10% of the activity observed with Mg2+
NaCl
-
optimal activity at 50 mM NaCl2
Zinc
-
a conserved zinc binding domain in the largest subunit of DNA-dependent RNA polymerase modulates intrinsic transcription termination and antitermination but does not stabilize the elongation complex
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
-
(S)-2-((1-amino-1-oxo-3-phenylpropan-2-ylamino)methyl)-3-(4-amino phenoxy)-5-methoxy phenyl acetate
(S)-2-((1-amino-1-oxo-3-phenylpropan-2-ylamino)methyl)-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
(S)-2-((1-amino-3-(4-hydroxyphenyl)-1-oxopropan-2-ylamino)methyl)-3-(4-aminophenoxy)-5-methoxyphenyl acetate
(S)-2-((1-amino-3-(4-hydroxyphenyl)-1-oxopropan-2-ylamino)methyl)-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
1,3-dimethoxy-5-(4-nitrophenoxy) benzene
1-(3-C-ethynyl-beta-D-ribo-pentofuranosyl)cytosine
-
i.e. ECyd, TAS-106, a antitumor ribonucleoside that inhibits RNA polymerase, acts synergistically in inhibiting A-549 cancer cell growth and in tumor growth in vivo. The compound also inhibits the checkpoint-associated protein, the expression of Chk1 protein and the phosphorylation of Chk1 and Chk2, antitumour effects in combination with cisplatin, overview
1-[2-[3-(4-Chloro-3-trifluoromethylphenyl)ureido]-4-trifluoromethyl phenoxy]-4,5-dichlorobenzene sulfonic acid
-
-
2,4-dimethoxy-6-(4-nitrophenoxy) benzaldehyde
2-([[(1S)-2-amino-1-(4-hydroxybenzyl)-2-oxoethyl]amino]methyl)-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
2-acetyl-3-hydroxy-5-methoxyphenyl acetate
2-acetyl-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
2-formyl-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
2-hydroxy-4-methoxy-6-(4-nitrophenoxy) benzaldehyde
3'-ethynylcytidine-5'-triphosphate
-
i.e. ECTP, competitive inhibition in the presence of isolated nuclei from FM3A mouse tumor cells
4-[2-([[(1S)-2-amino-1-(4-hydroxybenzyl)ethyl]amino]methyl)-5-methoxy-3-(2-oxopropyl)benzyl]benzaldehyde
ABI-1131
-
-
actinomycin
-
-
actinomycin D
-
-
alpha-amanithin
alpha-Amanitin
amanitin
-
-
aureolic acid
-
-
B2 RNA
-
the about 180-nt B2 RNA potently represses mRNA transcription by binding tightly to RNA polymerase II and assembling with it into complexes on promoter DNA, where it keeps the polymerase from properly engaging the promoter DNA. The C-terminal domain of the largest Pol II subunit is not involved. B2 RNA binds Pol II and assembles into complexes at promoters. Binding site anaylsis usig Pol II peptides, binding structure, and mechanism of transcriptional repression by B2 RNA, detailed overview
-
breast cancer susceptibility gene 1
-
BRCA1, inhibits RNA pol III via inhibition of the essential transcription factor TFIIIB, mechanism, overview. BRCA1 is a tumor suppressor playing a role in DNA repair, cell cycle regulation, apoptosis, genome integrity, and ubiquitination, and it BRCA1 has a conserved N-terminal RING domain, an activation domain 1, AD1, and an acidic C-terminal domain, BRCA1 C-terminal region. Interaction with TFIIIB occurs via the BRCA1 C-terminal region domain of Fcp1p, an RNA polymerase II phosphatase. RNA pol III inhibition involves the TFIIB family members Brf1 and Brf2, overview
-
CBR-703
-
-
CBR703
-
the IC50s values are significantly decreased with template Kool NC-45, or increased with template poly(dA-dT)
Cdc14
-
a protein phosphatase required for nucleolar segregation and mitotic exit4, inhibits RNA polymerase I, the phosphatase activity of Cdc14 is required for Pol I inhibition in vitro and in vivo involving nucleolar exclusion of Pol I subunits
-
chromomycin
-
-
-
Cinerubin B
-
-
cisplatin
corallopyronin
-
inhibition is not affected by template Kool NC-45
corallopyronin A
-
-
Cordycepin triphosphate
-
-
d(Ap4C)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
d(Ap4G)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
d(Ap4T)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
d(Tp4C)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
d(Tp4T)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
daunomycin
-
-
Echinomycin
-
-
Eruticulomycin A
-
-
-
Ethidium bromide
-
-
etnangien
etnangien methyl ester
etoposide
-
treatment with 0.02 mM etoposide leads to a transient inhibition of rRNA synthesis
Exotoxin of Bacillus thuringiensis
-
-
-
GE-23077-A
-
-
GE-23077-B
-
-
heparin
Isoquinocyclin
-
-
-
KCl
-
above 10 mM
lipiarmycin
-
-
MnCl2
-
in presence of 10 mM MgCl2
myxopyronin
myxopyronin A
-
-
Nogalamycin
-
-
Olivomycin
-
-
oxygen
-
the enzyme is highly oxygen sensitive. Inactivation is accompanied by cross-linking of components. Inactivated enzyme can be reactivated by reduction with sodium dithionite
procyclin-associated genes
-
i.e. PAG1, PAG2 or PAG3, inhibit RNA synthesis, deletion of PAGs lead to increased mRNA levels, regulation of PAG expressions, overview
-
proflavin sulfate
-
-
protein TLS
-
translocated in liposarcoma, a protein originally identified as the product of a chromosomal translocation, which associates with both RNAP II and the spliceosome, also represses transcription by RNAP III. It represses transcription from all three classes of RNAP III promoters in vitro and to associates with RNAP III genes in vivo. Depletion of TLS by siRNA in HeLa cells resulted in increased steady-state levels of RNAP III transcripts as well as increased RNAP III and TBP occupancy at RNAP III-transcribed genes
-
RBL-1
-
oligonucleotide, efficiently inhibits
-
RECQL5
-
a DNA helicase of the RECQ family, directly inhibits RNA polymerase II. It RECQL5 inhibits both initiation and elongation in transcription assays reconstituted with highly purified general transcription factors and RNAPII, RECQL5 helicase activity is not required for inhibition
-
rifabutin
-
-
rifalazil
-
-
rifampicin
ripostatin A
-
-
sorangicin A
-
-
Spt5
-
the large subunit of the DRB sensitivity-inducing factor, DSIF, represses or activates RNAPII elongation in vitro. CTR1 and CTR2CT, the two repeat-containing regions constituting the C-terminus of Spt5, play a redundant role in repressing RNAPII elongation in vivo, overview. Mutant NSpt5, lacking the C-terminus, directly associates with hsp70-4 chromatin in vivo and increases the occupancy of RNAPII, positive transcription elongation factor b, histone H3 Lys 4 trimethylation, and surprisingly, the negative elongation factor A at the locus, indicating a direct action of NSpt5 on the elongation repressed locus, nuclear extracts containing the constitutively active P-TEFb and WT DSIF lead to a time-dependent increase of the long, promoter-distal RNase T1-resistant products, reflecting the elongation stimulatory activity of Spt5, overview
-
Streptolydigin
streptovaracin
-
-
-
streptovaricin
-
-
Tagetitoxin
TFAM
-
DNA packaging by TFAM makes the DNA more resistant to unwinding
-
ureidothiophene
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ctk1
-
the kinase is required for the stability of the scaffold, but Ctk1 kinase activity is not required for the dissociation of basal transcription factors
-
glutamate
-
glutamate remodels the sigma38 transcription complex for activation. Accumulation of the simple signaling molecule glutamate can reprogram RNA polymerase in vitro without the need for specific protein receptors. During osmotic activation, glutamate appears to act as a Hofmeister series osmolyte to facilitate promoter escape. Escape is accompanied by a remodeling of the key interaction between the sigma38 stress protein and the beta-flap of the bacterial core RNA polymerase. This activation event contrasts with the established mechanism of inhibition in which glutamate, by virtue of its electrostatic properties, helps to inhibit binding to ribosomal promoters after osmotic shock
potassium acetate
-
activates
potassium aspartate
-
activates
potassium chloride
-
activates
potassium glutamate
-
activates highly, role of potassium ion in the activation of osmotic transcription
potassium nitrate
-
activates
RNase P
-
required for Pol I and Pol III required
-
sigma
-
a dissociable specificity sigma factor, regulated by factors such as anti-sigma factors, which can sequester r factors and prevent core association, and possibly by factors that enhance sigma-core association
-
sigma factor
sigma70
-
the sigma factor increases the transcription efficiency of templates with nonphysiological nonprokaryotic promoters
-
spermidine
Spt5
-
the large subunit of the DRB sensitivity-inducing factor, DSIF, represses or activates RNAPII elongation in vitro. CTR1 and CTR2CT, the two repeat-containing regions constituting the C-terminus of Spt5, play a redundant role in repressing RNAPII elongation in vivo, overview. Mutant NSpt5, lacking the C-terminus, directly associates with hsp70-4 chromatin in vivo and increases the occupancy of RNAPII, positive transcription elongation factor b, histone H3 Lys 4 trimethylation, and surprisingly, the negative elongation factor A at the locus, indicating a direct action of NSpt5 on the elongation repressed locus, nuclear extracts containing the constitutively active P-TEFb and WT DSIF lead to a time-dependent increase of the long, promoter-distal RNase T1-resistant products, reflecting the elongation stimulatory activity of Spt5, overview
-
Spt6
-
transcription factor, Pol II shows a broad requirement for essential Spt6 during different stages of development, e.g. for for maximal recruitment of Paf1 and Spt5 to transcriptionally active Hsp70. Spt6 interacts with both nucleosome structure and Pol II, it has a role in elongation, directed RNAi knock-down of Spt6 reduces the elongation rate, the Spt6-dependent effect on elongation rate persists during steady-state-induced transcription, reducing the elongation rate from about 1100 to 500 bp/min. Stimulation of Pol II elongation rate by Spt6 is not mediated through transcription factor TFIIS
-
TAFI protein
TFB2
-
the essential initiation factor forms a network of interactions with DNA near the transcription start site and facilitates promoter melting but may not be essential for promoter recognition, TFB2 bridges upstream and downstream promoter contacts of the initiation complex, mapping of TFB2-DNA interactions at the transcription start site, overview
-
TFB2M
-
the requirement for TFB2M in transcription of dsDNA is that it can stabilize an incompletely single-stranded template established by negative supercoiling
-
TFIIIE
-
a basal transcription factor, complexes with several ribosomal proteins and enhances tRNA and 5S rRNA transcription of the RNA polymerase, regualtion, overview
-
TFIIS
-
an RNA cleavage stimulatory factor TFIIS. TFIIS can rescue an arrested polymerase by creating a new RNA 3' end at the active site from which transcription can resume, mechanism, overview
-
thermine
-
optimal activity at pH 8.5 is obtained in presence of 18 mM MgCl2, 200 mM KCl, 1 mM thermine and 1 mM spermidine
TIF-IB/SL 1
transcription factor TFIIIB
-
proper initiation by RNA pol III requires the transcription factor TFIIIB. Gene-external U6 snRNA transcription requires TFIIIB consisting of Bdp1, TBP, and Brf2. Transcription from the gene internal tRNA promoter requires TFIIIB composed of Bdp1, TBP, and Brf1. Breast cancer susceptibility gene 1, BRCA1, inhibits TFIIB, which interacts with the BRCA1 C-terminal region domain of Fcp1p, an RNA polymerase II phosphatase, TFIIIB regulation network, overview
-
upstream binding factor
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018
A10G2A2C2C
-
-
-
0.041
A9G3A2C2C
-
-
-
0.0095 - 0.384
ATP
0.143 - 0.18
CTP
-
wild-type enzyme
0.00211
d(Ap4T)
-
-
-
0.00222
d(Tp4C)
-
-
-
0.00174
d(Tp4G)
-
-
-
0.00072
d(Tp4T)
-
-
-
0.015 - 1.4
dGTP
0.0004
dTTP
-
-
1.2 - 1.7
dUTP
0.043
G2CAC2C
-
-
-
0.1 - 0.234
GTP
0.000016
promoter complex
-
-
-
0.0103 - 0.32
rGTP
0.036 - 0.041
rUTP
0.0018
T10G2T2C2C
-
-
-
0.079 - 0.107
UTP
-
wild-type enzyme
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.26
A10G2A2C2C
Enterobacteria phage T7
-
-
-
4
A9G3A2C2C
Enterobacteria phage T7
-
-
-
0.00065 - 0.482
ATP
0.1 - 0.34
dGTP
0.03
G2CAC2C
Enterobacteria phage T7
-
-
-
8.3
promoter complex
Enterobacteria phage T7
-
-
-
0.25 - 0.38
rGTP
0.23
T10G2T2C2C
Enterobacteria phage T7
-
-
-
additional information
additional information
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000343
-
-
0.006
-
-
0.0156
-
-
4600
purified recombinant His6-tagged enzyme, pH 7.9, 37C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
Tris-HCl buffer
9
-
glycine-NaOH buffer
10
-
poly(dA-dT) DNA as a template
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.7
-
pH 7.0: about 75% of maximal activity, pH 8.7: about 75% of maximal activity
7 - 9
-
pH 7.0: about 30% of maximal activity, pH 9.0: about 60% of maximal activity
7.5 - 9
80% of maximall activity within this range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
assay at
37
-
assay at
40 - 45
-
with poly(dA-dT) DNA or Clostridium acetobutylicum DNA as template
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
activity range, profile, overview
30 - 50
-
30C: about 50% of maximal activity, 50C: about 20% of maximal activity
37 - 50
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
stably transfected with plasmid RPB3-SPB
Manually annotated by BRENDA team
-
in embryonic stem cells, silent developmental regulator genes that are repressed by Polycomb are associated with a form of RNAPII that can elongate through coding regions but that lacks the post-translational modifications that are important for coupling RNA synthesis to co-transcriptional maturation
Manually annotated by BRENDA team