Information on EC 2.7.7.6 - DNA-directed RNA polymerase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.7.6
-
RECOMMENDED NAME
GeneOntology No.
DNA-directed RNA polymerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
nucleoside triphosphate + RNAn = diphosphate + RNAn+1
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Purine metabolism
-
-
Pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
nucleoside-triphosphate:RNA nucleotidyltransferase (DNA-directed)
Catalyses DNA-template-directed extension of the 3'- end of an RNA strand by one nucleotide at a time. Can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. See also EC 2.7.7.19 (polynucleotide adenylyltransferase) and EC 2.7.7.48 (RNA-directed RNA polymerase).
CAS REGISTRY NUMBER
COMMENTARY hide
9014-24-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
beta-subunit; gene rpoB, Catharanthus roseus from Maryland, USA
UniProt
Manually annotated by BRENDA team
beta-subunit; gene rpoB, Catharanthus roseus from Maryland, USA
UniProt
Manually annotated by BRENDA team
subunit 6 and subunit beta
Q5UQ32 AND E3VZD2
UniProt
Manually annotated by BRENDA team
PCC 7120
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Arachis hypogaea phytoplasma
beta-subunit; gene rpoB, Arachis hypogaea from China
UniProt
Manually annotated by BRENDA team
Arachis hypogaea phytoplasma Peanut witches-broom
beta-subunit; gene rpoB, Arachis hypogaea from China
UniProt
Manually annotated by BRENDA team
Asclepias syriaca phytoplasma Milkweed yellows
Autographa californica M nucleopolyhedrovirus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Capsicum annum phytoplasma
beta-subunit; gene rpoB, Capsicum annum from Serbia
UniProt
Manually annotated by BRENDA team
Capsicum annum phytoplasma Candidatus Phytoplasma solani
beta-subunit; gene rpoB, Capsicum annum from Serbia
UniProt
Manually annotated by BRENDA team
beta-subunit; gene rpoB, Chrysanthemum coronarium from Japan
UniProt
Manually annotated by BRENDA team
Chrysanthemum coronarium phytoplasma Onion yellows
beta-subunit; gene rpoB, Chrysanthemum coronarium from Japan
UniProt
Manually annotated by BRENDA team
DSM 1731
-
-
Manually annotated by BRENDA team
RNA polymerase I, II and III
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene k1ep
UniProt
Manually annotated by BRENDA team
several strains, gene rpoA
-
-
Manually annotated by BRENDA team
beta-subunit; gene rpoB, Euphorbia pulcherrima from USA
UniProt
Manually annotated by BRENDA team
Euphorbia pulcherrima phytoplasma Poinsettia branch-inducing
beta-subunit; gene rpoB, Euphorbia pulcherrima from USA
UniProt
Manually annotated by BRENDA team
Fragaria x ananassa phytoplasma
beta-subunit; gene rpoB, Fragaria x ananassa from Lithuania
UniProt
Manually annotated by BRENDA team
Fragaria x ananassa phytoplasma Candidatus Phytoplasma fragariae
beta-subunit; gene rpoB, Fragaria x ananassa from Lithuania
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
beta-subunit; gene rpoB, Gomphocarpus physocarpus from Australia
UniProt
Manually annotated by BRENDA team
Gomphocarpus physocarpus phytoplasma Candidatus Phytoplasma australiense
beta-subunit; gene rpoB, Gomphocarpus physocarpus from Australia
UniProt
Manually annotated by BRENDA team
beta-subunit; gene rpoB, Heracleum sosnowskyi from Lithuania
UniProt
Manually annotated by BRENDA team
Heracleum sosnowskyi phytoplasma Heracleum yellows
beta-subunit; gene rpoB, Heracleum sosnowskyi from Lithuania
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Lactuca sativa phytoplasma
beta-subunit; gene rpoB, Lactuca sativa from Ohio, USA
UniProt
Manually annotated by BRENDA team
Lactuca sativa phytoplasma Aster yellows witches-broom
beta-subunit; gene rpoB, Lactuca sativa from Ohio, USA
UniProt
Manually annotated by BRENDA team
Lamium sp.
genes rpoA, rpoB, rpoC1, and rpoC2 encoding the enzyme subunits
-
-
Manually annotated by BRENDA team
strain MHOM/IL/81/Friedlin
-
-
Manually annotated by BRENDA team
strain MHOM/IL/81/Friedlin
-
-
Manually annotated by BRENDA team
strain UR6
-
-
Manually annotated by BRENDA team
strain UR6
-
-
Manually annotated by BRENDA team
Lycopersicon esculentum phytoplasma Tomato big bud
beta-subunit; gene rpoB, Lycopersicon esculentum from Arkansas, USA
UniProt
Manually annotated by BRENDA team
Lycopersicon esculentum phytoplasma Tomato yellows
beta-subunit; gene rpoB, Lycopersicon esculentum from Japan
UniProt
Manually annotated by BRENDA team
the nucleotide sequence of a part, 8334 bp, of the EcoRI fish lymphocystis disease virus DNA fragment B, between the EcoRI site and 259 nucleotides downstream from the second PstI site has been deposited in GenBank accession number: L34213
SwissProt
Manually annotated by BRENDA team
Malus domestica phytoplasma
beta-subunit; gene rpoB, Malus domestica from Germany
UniProt
Manually annotated by BRENDA team
Malus domestica phytoplasma Candidatus Phytoplasma mali
beta-subunit; gene rpoB, Malus domestica from Germany
UniProt
Manually annotated by BRENDA team
Malus domesticus phytoplasma
beta-subunit; gene rpoB, Malus domesticus from Germany
UniProt
Manually annotated by BRENDA team
Malus domesticus phytoplasma Ca. Phytoplasma mali
beta-subunit; gene rpoB, Malus domesticus from Germany
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
encoded on the linear mitochondrial plasmid
-
-
Manually annotated by BRENDA team
strain mc2155
-
-
Manually annotated by BRENDA team
strain mc2155
-
-
Manually annotated by BRENDA team
gene rpoA encoding subunit A
UniProt
Manually annotated by BRENDA team
two RNA polymerase paralogues rpoB(S) and rpoB(R)
-
-
Manually annotated by BRENDA team
Oenothera biennis phytoplasma
beta-subunit; gene rpoB, Oenothera biennis from Lithuania
UniProt
Manually annotated by BRENDA team
Oenothera biennis phytoplasma Oenothera biennis yellows
beta-subunit; gene rpoB, Oenothera biennis from Lithuania
UniProt
Manually annotated by BRENDA team
scorpionfly, collected in June in the village of Toksovo, Leningrad region, Russia
-
-
Manually annotated by BRENDA team
DNA-directed RNA polymerase II subunit RPB6 and subunit RPB10
R4THW7 AND R4TFI0
UniProt
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
Poa pratensis phytoplasma
beta-subunit; gene rpoB, Poa pratensis from Lithuania
UniProt
Manually annotated by BRENDA team
Poa pratensis phytoplasma Poa stunt
beta-subunit; gene rpoB, Poa pratensis from Lithuania
UniProt
Manually annotated by BRENDA team
Prunus persica phytoplasma
beta-subunit; gene rpoB, Prunus persica from Canada
UniProt
Manually annotated by BRENDA team
Prunus persica phytoplasma Canada peach X-disease
beta-subunit; gene rpoB, Prunus persica from Canada
UniProt
Manually annotated by BRENDA team
PpY101
-
-
Manually annotated by BRENDA team
strain WCS358
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain JB740 derivative of strain GRF167
-
-
Manually annotated by BRENDA team
strain yBC-10
-
-
Manually annotated by BRENDA team
single phage-type RNA polymerase gene rpoT
UniProt
Manually annotated by BRENDA team
strain DSS12, a deep-sea piezophilic bacterium, genes rpoE2 and rpoE3
-
-
Manually annotated by BRENDA team
strain DSS12, a deep-sea piezophilic bacterium, genes rpoE2 and rpoE3
-
-
Manually annotated by BRENDA team
Spiraea sp. phytoplasma
beta-subunit; gene rpoB, Spiraea sp. from New York, USA
UniProt
Manually annotated by BRENDA team
Spiraea sp. phytoplasma Spiraea stunt
beta-subunit; gene rpoB, Spiraea sp. from New York, USA
UniProt
Manually annotated by BRENDA team
Stellaria media phytoplasma
beta-subunit; gene rpoB, Stellaria media from Lithuania
UniProt
Manually annotated by BRENDA team
Stellaria media phytoplasma Chickweed yellows
beta-subunit; gene rpoB, Stellaria media from Lithuania
UniProt
Manually annotated by BRENDA team
Q980R2: subunit A', P58192: subunit A'', Q980R1: subunit B, P95989: subunit D, Q980A3: subunit E', Q9UXD9: subunit F, Q980L5: subunit G, Q980Q9: subunit H, Q97ZJ9: subunit K, Q980K0: subunit L, Q980Z8: subunit N, Q97ZX7: subunit P, Q980B8: subunit 13
Q980R2 and P58192 and Q980R1 and P95989 and Q980A3 and Q9UXD9 and Q980L5 and Q980Q9 and Q97ZJ9 and Q980K0 and Q980Z8 and Q97ZX7 and Q980B8
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene TON-0309
UniProt
Manually annotated by BRENDA team
Trifolium repens phytoplasma
beta-subunit; gene rpoB, Trifolium repens from Canada
UniProt
Manually annotated by BRENDA team
Trifolium repens phytoplasma Clover phyllody
beta-subunit; gene rpoB, Trifolium repens from Canada
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain WR
-
-
Manually annotated by BRENDA team
strain WR
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
UniProt
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
UniProt
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
gene rpoB encodes the RNA polymerase beta subunit
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Zea mays phytoplasma
beta-subunit; gene rpoB, Zeamays from Mexico
UniProt
Manually annotated by BRENDA team
Zea mays phytoplasma Maize bushy stunt
beta-subunit; gene rpoB, Zeamays from Mexico
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-C-methyl-ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
misincorporation frequency of approximately 1 in 7800 2'-C-methyl-ATP
-
-
?
2'-deoxy-ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
misincorporation
-
-
?
3'-deoxy-ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
misincorporation frequency of approximately 1 in 5 3'-dATP
-
-
?
ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
CTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
d(Ap4T) + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
d(TP4C) + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
d(Tp4G) + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
d(Tp4T) + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
DNA + 5-[[(2-aminoethyl)amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[(2-methylpropyl)amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[(2-pyridinylmethyl)amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[(4-pyridinylmethyl)amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[benzylamino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[[2-(1H-imidazol-4-yl)ethyl]amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
DNA + 5-[[[2-(1H-indol-3-yl)ethyl]amino]carbonyl]-UTP
?
show the reaction diagram
-
-
-
-
?
dTTP + RNAn
?
show the reaction diagram
-
primer elongation
-
-
?
GTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
nucleoside triphosphate + A10G2A2C2C
?
show the reaction diagram
-
oligonucleotide extension
-
-
?
nucleoside triphosphate + A9G3A2C2C
?
show the reaction diagram
-
oligonucleotide extension
-
-
?
nucleoside triphosphate + G2CAC2C
?
show the reaction diagram
-
oligonucleotide extension
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
show the reaction diagram
nucleoside triphosphate + T10G2T2C2C
?
show the reaction diagram
-
oligonucleotide extension
-
-
?
rNTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
CTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
GTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe-S cluster
-
with 4Fe-4S cluster-binding motif
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
can replace Mg2+ in activation, 10% of the activity observed with Mg2+
NaCl
-
optimal activity at 50 mM NaCl2
Zinc
-
a conserved zinc binding domain in the largest subunit of DNA-dependent RNA polymerase modulates intrinsic transcription termination and antitermination but does not stabilize the elongation complex
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
-
(S)-2-((1-amino-1-oxo-3-phenylpropan-2-ylamino)methyl)-3-(4-amino phenoxy)-5-methoxy phenyl acetate
(S)-2-((1-amino-1-oxo-3-phenylpropan-2-ylamino)methyl)-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
(S)-2-((1-amino-3-(4-hydroxyphenyl)-1-oxopropan-2-ylamino)methyl)-3-(4-aminophenoxy)-5-methoxyphenyl acetate
(S)-2-((1-amino-3-(4-hydroxyphenyl)-1-oxopropan-2-ylamino)methyl)-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
1,3-dimethoxy-5-(4-nitrophenoxy) benzene
1-(3-C-ethynyl-beta-D-ribo-pentofuranosyl)cytosine
-
i.e. ECyd, TAS-106, a antitumor ribonucleoside that inhibits RNA polymerase, acts synergistically in inhibiting A-549 cancer cell growth and in tumor growth in vivo. The compound also inhibits the checkpoint-associated protein, the expression of Chk1 protein and the phosphorylation of Chk1 and Chk2, antitumour effects in combination with cisplatin, overview
1-[2-[3-(4-Chloro-3-trifluoromethylphenyl)ureido]-4-trifluoromethyl phenoxy]-4,5-dichlorobenzene sulfonic acid
-
-
2,4-dimethoxy-6-(4-nitrophenoxy) benzaldehyde
2-([[(1S)-2-amino-1-(4-hydroxybenzyl)-2-oxoethyl]amino]methyl)-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
2-acetyl-3-hydroxy-5-methoxyphenyl acetate
2-acetyl-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
2-formyl-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
2-hydroxy-4-methoxy-6-(4-nitrophenoxy) benzaldehyde
3'-ethynylcytidine-5'-triphosphate
-
i.e. ECTP, competitive inhibition in the presence of isolated nuclei from FM3A mouse tumor cells
4-[2-([[(1S)-2-amino-1-(4-hydroxybenzyl)ethyl]amino]methyl)-5-methoxy-3-(2-oxopropyl)benzyl]benzaldehyde
6,10,6'-tri-O-tert-butyldimethylsilyl-6-epi-catalpol
-
-
ABI-1131
-
-
actinomycin
-
-
actinomycin D
-
-
alpha-amanithin
alpha-Amanitin
amanitin
-
-
aureolic acid
-
-
B2 RNA
-
the about 180-nt B2 RNA potently represses mRNA transcription by binding tightly to RNA polymerase II and assembling with it into complexes on promoter DNA, where it keeps the polymerase from properly engaging the promoter DNA. The C-terminal domain of the largest Pol II subunit is not involved. B2 RNA binds Pol II and assembles into complexes at promoters. Binding site anaylsis usig Pol II peptides, binding structure, and mechanism of transcriptional repression by B2 RNA, detailed overview
-
breast cancer susceptibility gene 1
-
BRCA1, inhibits RNA pol III via inhibition of the essential transcription factor TFIIIB, mechanism, overview. BRCA1 is a tumor suppressor playing a role in DNA repair, cell cycle regulation, apoptosis, genome integrity, and ubiquitination, and it BRCA1 has a conserved N-terminal RING domain, an activation domain 1, AD1, and an acidic C-terminal domain, BRCA1 C-terminal region. Interaction with TFIIIB occurs via the BRCA1 C-terminal region domain of Fcp1p, an RNA polymerase II phosphatase. RNA pol III inhibition involves the TFIIB family members Brf1 and Brf2, overview
-
CBR-703
-
-
CBR703
-
the IC50s values are significantly decreased with template Kool NC-45, or increased with template poly(dA-dT)
Cdc14
-
a protein phosphatase required for nucleolar segregation and mitotic exit4, inhibits RNA polymerase I, the phosphatase activity of Cdc14 is required for Pol I inhibition in vitro and in vivo involving nucleolar exclusion of Pol I subunits
-
chromomycin
-
-
-
Cinerubin B
-
-
cisplatin
corallopyronin
-
inhibition is not affected by template Kool NC-45
corallopyronin A
-
-
Cordycepin triphosphate
-
-
d(Ap4C)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
d(Ap4G)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
d(Ap4T)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
d(Tp4C)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
d(Tp4T)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
-
daunomycin
-
-
Echinomycin
-
-
Eruticulomycin A
-
-
-
Ethidium bromide
-
-
etnangien
etnangien methyl ester
etoposide
-
treatment with 0.02 mM etoposide leads to a transient inhibition of rRNA synthesis
Exotoxin of Bacillus thuringiensis
-
-
-
GE-23077-A
-
-
GE-23077-B
-
-
heparin
Isoquinocyclin
-
-
-
KCl
-
above 10 mM
lipiarmycin
-
-
ML-60218
-
treatment of A-549 cells with the Pol III inhibitor ML-60218 decreased the cytosolic RNA:DNA hybrid staining
-
MnCl2
-
in presence of 10 mM MgCl2
myxopyronin
myxopyronin A
-
-
Nogalamycin
-
-
Olivomycin
-
-
oxygen
-
the enzyme is highly oxygen sensitive. Inactivation is accompanied by cross-linking of components. Inactivated enzyme can be reactivated by reduction with sodium dithionite
per-O-acetyl-verbascoside
-
compound isolated from aerial part of Buddleja cordobensis Grisebach, most active among the compounds tested
procyclin-associated genes
-
i.e. PAG1, PAG2 or PAG3, inhibit RNA synthesis, deletion of PAGs lead to increased mRNA levels, regulation of PAG expressions, overview
-
proflavin sulfate
-
-
protein Rim1
-
the ssDNA-binding protein Rim1 severely inhibits theRNAsynthesis activity of Rpo41, but not the Rpo41-Mtf1 complex, which continues to prime DNA synthesis efficiently in the presence of Rim1
-
protein TLS
-
translocated in liposarcoma, a protein originally identified as the product of a chromosomal translocation, which associates with both RNAP II and the spliceosome, also represses transcription by RNAP III. It represses transcription from all three classes of RNAP III promoters in vitro and to associates with RNAP III genes in vivo. Depletion of TLS by siRNA in HeLa cells resulted in increased steady-state levels of RNAP III transcripts as well as increased RNAP III and TBP occupancy at RNAP III-transcribed genes
-
RBL-1
-
oligonucleotide, efficiently inhibits
-
RECQL5
-
a DNA helicase of the RECQ family, directly inhibits RNA polymerase II. It RECQL5 inhibits both initiation and elongation in transcription assays reconstituted with highly purified general transcription factors and RNAPII, RECQL5 helicase activity is not required for inhibition
-
rifabutin
-
-
rifalazil
-
-
rifampicin
ripostatin A
-
-
sorangicin A
-
-
Spt5
-
the large subunit of the DRB sensitivity-inducing factor, DSIF, represses or activates RNAPII elongation in vitro. CTR1 and CTR2CT, the two repeat-containing regions constituting the C-terminus of Spt5, play a redundant role in repressing RNAPII elongation in vivo, overview. Mutant NSpt5, lacking the C-terminus, directly associates with hsp70-4 chromatin in vivo and increases the occupancy of RNAPII, positive transcription elongation factor b, histone H3 Lys 4 trimethylation, and surprisingly, the negative elongation factor A at the locus, indicating a direct action of NSpt5 on the elongation repressed locus, nuclear extracts containing the constitutively active P-TEFb and WT DSIF lead to a time-dependent increase of the long, promoter-distal RNase T1-resistant products, reflecting the elongation stimulatory activity of Spt5, overview
-
Streptolydigin
streptovaracin
-
-
-
streptovaricin
-
-
Tagetitoxin
terminatin factor NsiI
-
N-terminally FLAG-tagged fusion protein Nsi1 expressed from Sf21 insect cells. Binding of the termination factor Nsi1 to its cognate DNA site is sufficient to terminate RNA polymerase I transcription in vitro and to induce termination in vivo. Nsi1 contains Myb-like DNA binding domains and associates in vivo near the 3' end of rRNA genes to rDNA. Binding of Nsi1 to a stretch of 11 nucleotides in the correct orientation is sufficient to pause elongating Pol I shortly upstream of the Nsi1 binding site and to release the transcripts in vitro, and the same minimal DNA element triggers Nsi1-dependent termination of pre-rRNA synthesis in vivo. Termination efficiency in the in vivo system can be enhanced by inclusion of specific DNA sequences downstream of the Nsi1 binding site
-
TFAM
-
DNA packaging by TFAM makes the DNA more resistant to unwinding
-
ureidothiophene
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ctk1
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the kinase is required for the stability of the scaffold, but Ctk1 kinase activity is not required for the dissociation of basal transcription factors
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glutamate
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glutamate remodels the sigma38 transcription complex for activation. Accumulation of the simple signaling molecule glutamate can reprogram RNA polymerase in vitro without the need for specific protein receptors. During osmotic activation, glutamate appears to act as a Hofmeister series osmolyte to facilitate promoter escape. Escape is accompanied by a remodeling of the key interaction between the sigma38 stress protein and the beta-flap of the bacterial core RNA polymerase. This activation event contrasts with the established mechanism of inhibition in which glutamate, by virtue of its electrostatic properties, helps to inhibit binding to ribosomal promoters after osmotic shock
histone-like nucleoid structuring