Cloned (Comment) | Organism |
---|---|
stable ectopic expression of subunit RPC32alpha in IMR90 fibroblasts | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | suppression of subunit RPC32alpha expression in HeLa cells by siRNAs. Levels of p53 and lamin A/C are dramatically downregulated by ectopic RPC32alpha expression, but either unchanged (lamin A/C) or moderately increased (p53) by ectopic RPC32beta subunit expression | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | isozyme Pol IIIalpha and Pol IIIbeta, primarily | Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | RNA polymerase III transcribes small untranslated RNAs that include tRNAs, 5S RNA, U6 RNA, and some microRNAs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | IMR90 fibroblasts | Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | RNA polymerase III transcribes small untranslated RNAs that include tRNAs, 5S RNA, U6 RNA, and some microRNAs | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pol III | - |
Homo sapiens |
Pol IIIalpha | - |
Homo sapiens |
Pol IIIbeta | - |
Homo sapiens |
RNA polymerase III | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | suppression of subunit RPC32alpha expression by siRNAs impedes anchorage-independent growth of HeLa cells, whereas ectopic expression of RPC32alpha in IMR90 fibroblasts enhances cell transformation and dramatically changes the expression of several tumor-related mRNAs and that of a subset of Pol III RNAs | Homo sapiens |
physiological function | increased Pol III transcription accompanies or causes cell transformation. RPC32beta subunit-containing isozyme Pol IIIbeta is ubiquitously expressed and essential for growth of human cells. RPC32alpha subunit-containing isozyme Pol IIIalpha is dispensable for cell survival, with expression being restricted to undifferentiated ES cells and to tumor cells. Dramatic changes in 5S RNA, U6 RNA, and 7SKRNA expression are specifically caused by ectopic expression of RPC32alpha and not due to a general deregulation of transcription | Homo sapiens |