Cloned (Comment) | Organism |
---|---|
the genes rpoA, rpoB and rpoC which encode the RNA polymerase, alpha-, beta- and betasubunits, respectively, have been individually placed on expression plasmids under control of the bacteriophage T7 promoter. Induction of the T7 RNA polymerase gene in hist cells harbouring each of the three plasmids, results in the extensive overproduction of the three polypeptides | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Escherichia coli | diphosphate + RNAn+1 | - |
? |