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Literature summary for 2.7.7.6 extracted from

  • Zalenskaya, K.; Lee, J.; Gujuluva, C.N.; Shin, Y.K.; Slutsky, M.; Goldfarb, A.
    Recombinant RNA polymerase: inducible overexpression, purification and assembly of Escherichia coli rpo gene products (1990), Gene, 89, 7-12.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the genes rpoA, rpoB and rpoC which encode the RNA polymerase, alpha-, beta- and beta‘subunits, respectively, have been individually placed on expression plasmids under control of the bacteriophage T7 promoter. Induction of the T7 RNA polymerase gene in hist cells harbouring each of the three plasmids, results in the extensive overproduction of the three polypeptides Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Purification (Commentary)

Purification (Comment) Organism
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Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn the enzyme requires DNA as template Escherichia coli diphosphate + RNAn+1
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