Cloned (Comment) | Organism |
---|---|
expression of C-terminally His-tagged alpha subunit-encoded by gene rpoA, and coexpression of the subunits of Xanthomonas campestris pv. campestris core RNAP from plasmid pBBad22K in Escherichia coli strain BL21(DE3), overexpression with or without omega | Xanthomonas campestris |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant Xcc core RNAP lacking omega shows a 13fold decrease in enzymatic activity in comparison with that containing omega. Promoter-specific transcription assays by recombinant Xcc core RNAP reconstituted with external added sigma factor show that the absence of omega debilitates the transcriptional activity of Xcc RNAP | Xanthomonas campestris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Xanthomonas campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | O08348 | rpoD; pv. campestris | - |
Xanthomonas campestris | P0A0Y1 | rpoA, alpha-subunit; pv. campestris | - |
Xanthomonas campestris | Q8PC55 | rpoC; pv. campestris | - |
Xanthomonas campestris | Q8PC56 | rpoB; pv. campestris | - |
Purification (Comment) | Organism |
---|---|
recombinant Xcc core RNAP subunits from Escherichia coli by a two-step chromatographic process | Xanthomonas campestris |
Renatured (Comment) | Organism |
---|---|
assembly of Xcc core RNAP subunits recombinantly coexpressed in Escherichia coli after purification by a two-step chromatographic process | Xanthomonas campestris |
assembly of Xcc core RNAP subunits recombinantly coexpressed in Escherichia coli after purification by a two-step chromatographic process including nickel affinity chromatography | Xanthomonas campestris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant Xcc core RNAP lacking omega shows a 13fold decrease in enzymatic activity in comparison with that containing omega | Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in vitro transcriptional activity of recombinant assembled Xcc RNAP, overview | Xanthomonas campestris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA polymerase | - |
Xanthomonas campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Xanthomonas campestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Xanthomonas campestris |