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Literature summary for 2.7.7.6 extracted from

  • Prangishvilli, D.; Zillig, W.; Gierl, A.; Biesert, L.; Holz, I.
    DNA-dependent RNA polymerase of thermoacidophilic archaebacteria (1982), Eur. J. Biochem., 122, 471-477.
    View publication on PubMed
Search for more literature for 2.7.7.6

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by 0.1 mg/ml of rifampicin, streptolydigin or alpha-amanitin Desulfurococcus mucosus
additional information no inhibition by 0.1 mg/ml of rifampicin, streptolydigin or alpha-amanitin Sulfolobus acidocaldarius
additional information no inhibition by 0.1 mg/ml of rifampicin, streptolydigin or alpha-amanitin Thermoproteus tenax
oxygen the enzyme is highly oxygen sensitive. Inactivation is accompanied by cross-linking of components. Inactivated enzyme can be reactivated by reduction with sodium dithionite Thermoproteus tenax

Metals/Ions

Metals/Ions Comment Organism Structure
K+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Sulfolobus acidocaldarius
K+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Thermoproteus tenax
K+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Desulfurococcus mucosus
Mg2+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Sulfolobus acidocaldarius
Mg2+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Thermoproteus tenax
Mg2+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Desulfurococcus mucosus

Organism

Organism UniProt Comment Textmining
Desulfurococcus mucosus
-
-
-
Desulfurococcus mucosus 07
-
-
-
Sulfolobus acidocaldarius
-
-
-
Sulfolobus acidocaldarius DSM 639
-
-
-
Thermoproteus tenax
-
-
-
Thermoproteus tenax DSM 2078
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is highly oxygen sensitive. Inactivation is accompanied by cross-linking of components. Inactivated enzyme can be reactivated by reduction with sodium dithionite Thermoproteus tenax

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius
-
 Thermoproteus tenax
-
 Desulfurococcus mucosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn DNA-template dependent reaction. T7 DNA and the plasmid PBR322 are by far the best templates. P2, T4 and T5 DNA are weak templates Desulfurococcus mucosus diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn DNA-template dependent reaction. The best of the templates is phiH DNA, whereas T7 and T4 DNA are comparatively inactive and P2 DNA is a very weak template Sulfolobus acidocaldarius diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn DNA-template dependent reaction. The enzyme transcribes phiH DNA as efficiently and T4 DNA as weakly as the Sulfolobus enzyme but T7 DNA even better than phiH DNA Thermoproteus tenax diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn DNA-template dependent reaction. The best of the templates is phiH DNA, whereas T7 and T4 DNA are comparatively inactive and P2 DNA is a very weak template Sulfolobus acidocaldarius DSM 639 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn DNA-template dependent reaction. The enzyme transcribes phiH DNA as efficiently and T4 DNA as weakly as the Sulfolobus enzyme but T7 DNA even better than phiH DNA Thermoproteus tenax DSM 2078 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn DNA-template dependent reaction. T7 DNA and the plasmid PBR322 are by far the best templates. P2, T4 and T5 DNA are weak templates Desulfurococcus mucosus 07 diphosphate + RNAn+1
-
 ?

Subunits

Subunits Comment Organism
More components of the RNA polymerase and their molecular weights determined by SDS-PAGE Sulfolobus acidocaldarius
More components of the RNA polymerase and their molecular weights determined by SDS-PAGE Thermoproteus tenax
More components of the RNA polymerase and their molecular weights determined by SDS-PAGE Desulfurococcus mucosus

Synonyms

Synonyms Comment Organism
DNA-dependent RNA polymerase
-
 Sulfolobus acidocaldarius
DNA-dependent RNA polymerase
-
 Thermoproteus tenax
DNA-dependent RNA polymerase
-
 Desulfurococcus mucosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 assay at Sulfolobus acidocaldarius
85
-
 assay at Thermoproteus tenax
85
-
 assay at Desulfurococcus mucosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Sulfolobus acidocaldarius
8
-
 assay at Thermoproteus tenax
8
-
 assay at Desulfurococcus mucosus
8 8.5
-
 Sulfolobus acidocaldarius
8 8.5
-
 Thermoproteus tenax
8 8.5
-
 Desulfurococcus mucosus