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Literature summary for 2.7.7.6 extracted from

  • Pich, A.; Baghl, H.
    Purification and characterization of the DNA-dependent RNA polymerase from Clostridium acetobutylicum (1991), J. Bacteriol., 173, 2120-2124.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
40% glycerol is absolutely required for stability Clostridium acetobutylicum

Inhibitors

Inhibitors Comment Organism Structure
rifampicin 0.1 mg/ml, complete inhibition Clostridium acetobutylicum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Mg2+ in activation, 10% of the activity observed with Mg2+ Clostridium acetobutylicum
Mg2+ highest activity is obtained with 20 mM Mg2+ on poly(dA-dT) DNA or Clostridium acetobutylicum DNA as template, with calf thymus DNA as template maximal activity is achieved with 10 mM Mg2+ Clostridium acetobutylicum
Mn2+ 3 mM Mn2+ results in comparable activity with poly(dA-dT) DNA but reduced activity on calf thymus DNA or Clostridium acetobutylicum DNA and Clostridium acetobutylicum DNA as substrated, compared to Mg2+ activition Clostridium acetobutylicum

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
DSM 1731
-

Purification (Commentary)

Purification (Comment) Organism
-
Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0527
-
-
Clostridium acetobutylicum

Storage Stability

Storage Stability Organism
-20°C, 60 mM Tris-HCl buffer, pH 7.8, 20 mM NH4Cl, 1 mM EDTA, 10 mM 2-mercaptoethanol, 40% glycerol, stable for several months Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn absolutely dependent on the presence of a double-stranded or single-stranded DNA template Clostridium acetobutylicum diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn with poly(dA-dT) DNA as template Clostridium acetobutylicum diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn the enzyme requires DNA as template Clostridium acetobutylicum diphosphate + RNAn+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45 with poly(dA-dT) DNA or Clostridium acetobutylicum DNA as template Clostridium acetobutylicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
poly(dA-dT) DNA as a template Clostridium acetobutylicum