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Literature summary for 2.7.7.6 extracted from
- Purification and characterization of a DNA-dependent RNA polymerase from Pseudomonas putida (1992), Biosci. Biotechnol. Biochem., 56, 1797-1800.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| rifampicin | 0.00006 mg/ml, 50% inactivation | Pseudomonas putida |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activity in presence of 5-10 mM MgCl2 | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
| 87000 | - |
betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
| 151000 | - |
betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
| 156000 | - |
betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
PpY101 | - |
| Pseudomonas putida PpY101 | - |
PpY101 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Pseudomonas putida | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Pseudomonas putida PpY101 | diphosphate + RNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | betax,betax,alphax,sigmax, x * 156000 + x * 151000 + x * 87000 + x * 42000, SDS-PAGE | Pseudomonas putida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 50 | 37°C: about 50% of maximal activity, 50°C: about 80% of maximal activity | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
10 min, stable | Pseudomonas putida |
| 40 | - |
10 min, slight inactivation | Pseudomonas putida |
| 50 | - |
10 min, slight inactivation | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7.0: about 30% of maximal activity, pH 9.0: about 60% of maximal activity | Pseudomonas putida |
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