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Literature summary for 2.7.7.6 extracted from

  • Jovcic, B.; Bertani, I.; Venturi, V.; Topisirovic, L.; Kojic, M.
    5' Untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation (2008), J. Microbiol., 46, 56-61.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Production of different translational and transcriptional fusions in Escherichia coli. PCR fragments of different sizes are cloned into plasmids resulting in intermediate constructs. Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
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Pseudomonas putida WCS358
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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15.4% activity in rpoS-lacZ2 translational fusion PMP77F4 (delta 50 bp), relative to pMP77FK Pseudomonas putida
additional information
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15.5% activity in rpoS-lacZ2 translational fusion PMP77F6 (delta 150 bp), relative to pMP77FK Pseudomonas putida
additional information
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23.3% activity in rpoS-lacZ2 translational fusion PMP77FdeltaEI (delta 31 bp) , relative to pMP77FK Pseudomonas putida
additional information
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35.8% activity in rpoS-lacZ2 translational fusion PMP77F5 (delta 100 bp), relative to pMP77FK Pseudomonas putida
additional information
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36.1% activity in rpoS-lacZ2 translational fusion PMP77F7 (delta 200 bp), relative to pMP77FK Pseudomonas putida
additional information
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55.7% activity in rpoS-lacZ2 translational fusion PMP77FdeltaE47III (delta 32 bp), relative to pMP77FK Pseudomonas putida
additional information
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77.7% activity in rpoS-lacZ2 translational fusion PMP77F3 (relative to pMP77FK) Pseudomonas putida
additional information
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78.2% activity in rpoS-lacZ2 translational fusion PMP77FINEI (insertion 78 bp), relative to pMP77FK Pseudomonas putida

Subunits

Subunits Comment Organism
More subunit RpoS of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genesin gram-negative bacteria. Pseudomonas putida

Synonyms

Synonyms Comment Organism
RNA polymerase
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Pseudomonas putida
RpoS subunit sigma factor Pseudomonas putida