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Literature summary for 2.7.7.6 extracted from

  • Domecq, C.; Kireeva, M.; Archambault, J.; Kashlev, M.; Coulombe, B.; Burton, Z.F.
    Site-directed mutagenesis, purification and assay of Saccharomyces cerevisiae RNA polymerase II (2010), Protein Expr. Purif., 69, 83-90.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.6

Cloned(Commentary)

Cloned (Comment) Organism
RPB2 gene encoding the Rpb2 subunit of yeast RNAP II, expression of mutant enzyme and subunits in Escherichia coli strain XL-1 Blue Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D505A mutation in subunit Rpb2, the mutant shows a weak defect in the escape from a transcriptional stall at A20 Saccharomyces cerevisiae
E1028Q mutation in subunit Rpb2, the mutant shows transcription elongation defects Saccharomyces cerevisiae
E529A mutation in subunit Rpb2, the mutant is faster in elongation compared to wild type RNAP II Saccharomyces cerevisiae
E529D mutation in subunit Rpb2, the mutant is faster in elongation compared to wild type RNAP II Saccharomyces cerevisiae
G985A/G987A the double substitution in subunit Rpb2 is expected to subtly affect the conformation and/or dynamics of K987, an essential residue Saccharomyces cerevisiae
K979Q lethal mutation in subunit Rpb2 Saccharomyces cerevisiae
K979R lethal mutation in subunit Rpb2 Saccharomyces cerevisiae
K987Q lethal mutation in subunit Rpb2 Saccharomyces cerevisiae
K987R lethal mutation in subunit Rpb2 Saccharomyces cerevisiae
additional information mutations are made in highly conserved residues Rpb2 K979, K987, and R1020 within the active site region of RNAP II, at positions that potentially might be essential for catalytic function. Mutations are constructed in an extended fork region of subunit Rpb2, residues R504-E529, construction of diverse mutant strains, e.g. strain yBC-9 expressing subunit Rpb2 under the control of the Gal promoter and is deleted for the chromosomal copy of the DST1 gene, encoding transcription factor IIS, TFIIS. From the strain yBC-9, the strain yBC-32 was constructed by transformation with pFL39-RPB2-TAP, TRP1 Saccharomyces cerevisiae
Q513A mutation in subunit Rpb2, the mutant shows a weak defect in the escape from a transcriptional stall at A20 Saccharomyces cerevisiae
R1020K lethal mutation in subunit Rpb2 Saccharomyces cerevisiae
R1020Q lethal mutation in subunit Rpb2 Saccharomyces cerevisiae
R512A mutation in subunit Rpb2, the mutant shows transcription elongation defects Saccharomyces cerevisiae
R512C mutation in subunit Rpb2, the mutant shows transcription elongation defects Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Saccharomyces cerevisiae 5634
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae yBC-10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant RNAP II proteins Saccharomyces cerevisiae

Subunits

Subunits Comment Organism
dodecamer the Rpb2 subunit of RNAP II combines with the Rpb1 subunit to form the RNAP II active site. RNAP II is isolated as a 12 subunit enzyme, Rpb1-Rpb12 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
RNA polymerase II
-
 Saccharomyces cerevisiae
RNAP II
-
 Saccharomyces cerevisiae