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3.4.22.28: picornain 3C

This is an abbreviated version!
For detailed information about picornain 3C, go to the full flat file.

Word Map on EC 3.4.22.28

Reaction

Selective cleavage of Gln-/-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly =

Synonyms

3C protease, 3C protein, 3C proteinase, 3C-like protease, 3C-protease, 3CL, 3cLpro, 3CP, 3Cpro, 3Cpro protease, Avihepatovirus 3C protease, coxsackievirus 3C proteinase, cysteine proteinase 3C, DHAV 3C protease, enterovirus 3C protease, EV71 3Cpro, foot-and-mouth protease 3C, HAC 3C, HAV 3C protease, HAV 3Cpro, hepatitis A virus 3C proteinase, HRV 3C protease, HRV 3Cpro, HRV type-14 3C protease, HRV16 3C protease, Human rhinovirus 3C protease, human rhinovirus type-14 3C protease, nonstructural 3 protease, NS3 protease, NS6 protease, P3C, picornain 3C, picornain-3C, picornaviral 3C protease, picornavirus endopeptidase 3C, poliovirus protease 3C, poliovirus proteinase 3C, protease 3C, proteinase, foot-and-mouth-disease virus, 3C, proteinase, picornavirus, 3C, proteinase, poliovirus, 3C, proteinase, rhinovirus, 3C, rhinovirus 3C protease, rhinovirus protease 3C, SAT-type 3C protease, Senecavirus A 3C protease, SV3CP, SVA 3Cpro, tomato ringspot nepovirus 3C-related protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.28 picornain 3C

Cloned

Cloned on EC 3.4.22.28 - picornain 3C

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 recombinant adenoviruses are constructed expressing foot-and-mouth disease virus capsid and 3C/3CD proteins in replicative deficient human adenovirus type 5 vector, production of adeno-FMD hybrid virus, about 50% of vaccinated pigs can be protected after virus challenge when the LPB-ELISA antibody titers are between 4 and 45
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a cDNA fragment containing the 3C protease coding region is inserted into pET22b vector and expressed in Escherichia coli
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cloned and expressed in Escherichia coli as a His-tagged fusion protein
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cloned into a plasmid under the control of the bacteriophage T7 promoter, expressed both in an in vito transcription-translation system and in vivo in an Escherichia coli system containig an inducible T7 RNA polymerase
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cloned into cDNA and expressed in Escherichia coli BL21 (DE3) pLysS, transfection of HeLa cells
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expressed in CEF cells infected with the recombinant fowlpox virus vUTAL3CP1 containing the 3C protease coding regions of foot-and-mouth-disease virus
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expressed in COS-7 cells
expressed in Escherichia coli
expressed in Escherichia coli BL2 1 (DE3) pLysS cells
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expressed in Escherichia coli BL21 (DE3) pLysS cells
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
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expressed in Escherichia coli or BL21(D3)
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expressed in Escherichia coli strain JM 109
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expressed in Escherichia coli Tuner (DE3) cells
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expressed in HEK-293T cells
expressed in HeLa cells
expressed with pET16b vector contained a N- or C-terminal hexa-His tag in Escherichia coli, protease with a C-terminus His-tag has specific activity equal to that of the tag-free enzyme, but the enzyme with N-terminal His-tag displays 10fold lower activity, indicating that additional N-terminal amino acids interfered with the protease activity
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expression as a fusion protein in Escherichia coli
expression in baby hamster kidney cells and human A293 cells
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expression in bacteria, cell-free transcription-translation systems and eukaryotic cells
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expression in BLR pLys DE3
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expression in Escherichia coli
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expression in Escherichia coli 21 (DE3)
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expression in Escherichia coli BL21 (DE3) pLysS
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expression in Escherichia coli BL21 (DE3) pLysS from a pET-28 vector modified to add a thrombin-cleavable N-terminal polyhistidine tag
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expression of 3C mutants in Escherichia coli
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green fluorescent protein-tagged enzyme is expressed in COS-7 cells
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into pET-23a and expressed in Escherichia coli BL21 (DE3 pLysS)
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into pQE30 and expressed in Escherichia coli M15 pREP4 strain
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overexpressed
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poliovirus His-3Cpro is expressed in Escherichia coli from pET-3C
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recombinant 3Cpro is expressed in Escherichia coli
recombinant expression of FLAG-tagged enzyme in HEK-293T cells
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recombinant expression of soluble GST-tagged enzyme in Escherichia coli strain BL21(DE3), optimization of the expression using response surface methodology
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recombinant expression of wild-type and mutant GFP-tagged or mCherry-tagged enzymes in Escherichia coli and in COS-7 cells
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recombinant overexpression of 3C protease, containing a His8-tag, an extended linker (MSTLESSGAASG), maltose binding protein (MBP), and a TEV protease cleavable site upstream from the multiple cloning site, in Escherichia coli strains Rosetta 2 (DE3) or BL21(DE3)
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SAT1/NIG/5/81 genotype enzyme, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS
SAT1/UGA/1/97 genotype enzyme, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS
SAT2/GHA/8/91 genotype enzyme, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS
SAT2/ZIM/7/83 genotype enzyme, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS
segment of poliovirus genome: nucleotides 5438-6061
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sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged DHAV 3C protease in Escherichia coli strain BL21, method optimization. Recombinant expression of N-terminally GFP-tagged wild-type enzyme and enzyme mutants in DEF cells with localization in the cytoplasm
YP_007969882
the 3Cpro mutant is produced heterologously using Escherichia coli BL21(DE3) pLysS containing pHAV-3CEX
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