Literature summary for 3.4.22.28 extracted from

Gouvea, I.E.; Judice, W.A.; Cezari, M.H.; Juliano, M.A.; Juhasz, T.; Szeltner, Z.; Polgar, L.; Juliano, L.
Kosmotropic salt activation and substrate specificity of poliovirus protease 3C (2006), Biochemistry, 45, 12083-12089.

Search for more literature for 3.4.22.28

Activating Compound

Activating Compound	Comment	Organism	Structure
acetate	activates	Enterovirus C
D2O	-	Enterovirus C
Sodium citrate	the peptidase activity of picornain-3C is 100fold higher in the presence of 1.5 M sodium citrate	Enterovirus C

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells	Enterovirus C

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	2-aminobenzoyl-EALFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.016	-	2-aminobenzoyl-EALFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.0179	-	2-aminobenzoyl-EALFQGPLQ-EDDnp	in D2O, in the absence of sodium citrate	Enterovirus C
0.0182	-	2-aminobenzoyl-AALFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.0185	-	2-aminobenzoyl-EALFQGPAQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.02	-	2-aminobenzoyl-AALFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.0203	-	2-aminobenzoyl-EALFQGPLQ-EDDnp	in D2O, in the presence of 1 M sodium citrate	Enterovirus C
0.0213	-	2-aminobenzoyl-EALFQGPAQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.0264	-	2-aminobenzoyl-EAAFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.0349	-	2-aminobenzoyl-EAAFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.0393	-	2-aminobenzoyl-EALFQAPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.0413	-	2-aminobenzoyl-EALAQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.0435	-	2-aminobenzoyl-EALAQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.0533	-	2-aminobenzoyl-EALFQGALQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cl-	activates	Enterovirus C
HPO42-	activates	Enterovirus C
SO42-	activates	Enterovirus C

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	in the presence of sodium citrate, gel filtration	Enterovirus C
22400	-	in the absence of sodium citrate, gel filtration	Enterovirus C

Organism

Organism	UniProt	Comment	Textmining
Enterovirus C	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Resource-Q column chromatography and a Superdex-75 gel filtration	Enterovirus C

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-aminobenzoyl-AALFQGPLQ-EDDnp + H2O	-	Enterovirus C	2-aminobenzoyl-AALFQ + GPLQ-EDDnp	-	?
2-aminobenzoyl-EAAFQGPLQ-EDDnp + H2O	-	Enterovirus C	2-aminobenzoyl-EAAFQ + GPLQ-EDDnp	-	?
2-aminobenzoyl-EALAQGPLQ-EDDnp + H2O	-	Enterovirus C	2-aminobenzoyl-EALAQ + GPLQ-EDDnp	-	?
2-aminobenzoyl-EALFQAPLQ-EDDnp + H2O	-	Enterovirus C	2-aminobenzoyl-EALFQ + APLQ-EDDnp	-	?
2-aminobenzoyl-EALFQGALQ-EDDnp + H2O	-	Enterovirus C	2-aminobenzoyl-EALFQ + GALQ-EDDnp	-	?
2-aminobenzoyl-EALFQGPAQ-EDDnp + H2O	-	Enterovirus C	2-aminobenzoyl-EALFQ + GPAQ-EDDnp	-	?
2-aminobenzoyl-EALFQGPLQ-EDDnp + H2O	-	Enterovirus C	2-aminobenzoyl-EALFQ + GPLQ-EDDnp	-	?
additional information	does not hydrolyze 4-aminobenzyl-EALFAGPLQED-2,4-dinitrophenol, 4-aminobenzyl-EALFEGPLQED-2,4-dinitrophenol, 4-aminobenzyl-EALFRGPLQED-2,4-dinitrophenol, 4-aminobenzyl-EALFHGPLQED-2,4-dinitrophenol, 4-aminobenzyl-EALFNGPLQED-2,4-dinitrophenol, and 4-aminobenzyl-EALFYGPLQED-2,4-dinitrophenol	Enterovirus C	?	-	?

Synonyms

Synonyms	Comment	Organism
picornain-3C	-	Enterovirus C

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	25	-	Enterovirus C

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	2-aminobenzoyl-EALAQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.01	-	2-aminobenzoyl-AALFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.03	-	2-aminobenzoyl-EALFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.03	-	2-aminobenzoyl-EALFQGPAQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.06	-	2-aminobenzoyl-EALFQGPLQ-EDDnp	in D2O, in the absence of sodium citrate	Enterovirus C
0.08	-	2-aminobenzoyl-EAAFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the absence of sodium citrate	Enterovirus C
0.28	-	2-aminobenzoyl-EALFQGALQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.34	-	2-aminobenzoyl-EALFQAPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.66	-	2-aminobenzoyl-EALAQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.87	-	2-aminobenzoyl-EALFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
0.96	-	2-aminobenzoyl-AALFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
2.02	-	2-aminobenzoyl-EALFQGPLQ-EDDnp	in D2O, in the presence of 1 M sodium citrate	Enterovirus C
2.72	-	2-aminobenzoyl-EALFQGPAQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C
3.34	-	2-aminobenzoyl-EAAFQGPLQ-EDDnp	in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate	Enterovirus C

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Release 2023.1 (January 2023)