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3.4.22.28: picornain 3C

This is an abbreviated version!
For detailed information about picornain 3C, go to the full flat file.

Word Map on EC 3.4.22.28

Reaction

Selective cleavage of Gln-/-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly =

Synonyms

3C protease, 3C protein, 3C proteinase, 3C-like protease, 3C-protease, 3CL, 3cLpro, 3CP, 3Cpro, 3Cpro protease, Avihepatovirus 3C protease, coxsackievirus 3C proteinase, cysteine proteinase 3C, DHAV 3C protease, enterovirus 3C protease, EV71 3Cpro, foot-and-mouth protease 3C, HAC 3C, HAV 3C protease, HAV 3Cpro, hepatitis A virus 3C proteinase, HRV 3C protease, HRV 3Cpro, HRV type-14 3C protease, HRV16 3C protease, Human rhinovirus 3C protease, human rhinovirus type-14 3C protease, nonstructural 3 protease, NS3 protease, NS6 protease, P3C, picornain 3C, picornain-3C, picornaviral 3C protease, picornavirus endopeptidase 3C, poliovirus protease 3C, poliovirus proteinase 3C, protease 3C, proteinase, foot-and-mouth-disease virus, 3C, proteinase, picornavirus, 3C, proteinase, poliovirus, 3C, proteinase, rhinovirus, 3C, rhinovirus 3C protease, rhinovirus protease 3C, SAT-type 3C protease, Senecavirus A 3C protease, SV3CP, SVA 3Cpro, tomato ringspot nepovirus 3C-related protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.28 picornain 3C

KM Value

KM Value on EC 3.4.22.28 - picornain 3C

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0431
(2-(N-methylamino)benzoyl)-IEALFQGPPK(2,4-dinitrophenyl)-FR
in 50 mM Tris, pH 7.0, at 30°C
0.00018
(E)-5-chloropyridin-3-yl 3-(furan-2-yl)acrylate
-
-
0.0735
2-(N-methylamino)benzoyl-DEAMEQGVSDYIK-2,4-dinitrophenyl
in 50 mM Tris, pH 7.0, at 30°C
0.075
2-(N-methylamino)benzoyl-IAALFQGPPK(2,4-dinitrophenyl)FR
in 50 mM Tris, pH 7.0, at 30°C
0.0667
2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR
in 50 mM Tris, pH 7.0, at 30°C
0.0482
2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR
in 50 mM Tris, pH 7.0, at 30°C
0.0924
2-(N-methylamino)benzoyl-RQAVTQGFPTELK-2,4-dinitrophenyl
in 50 mM Tris, pH 7.0, at 30°C
0.0582
2-(N-methylamino)benzoyl-RTATVQGPSLDFK-2,4-dinitrophenyl
in 50 mM Tris, pH 7.0, at 30°C
0.075
2-(N-methylamino)benzoyl-TSAVLQSGFRK-(2,4-dinitrophenyl)-Met
in 50 mM Tris, pH 7.0, at 30°C
0.0182 - 0.02
2-aminobenzoyl-AALFQGPLQ-EDDnp
0.0264 - 0.0349
2-aminobenzoyl-EAAFQGPLQ-EDDnp
0.0413 - 0.0435
2-aminobenzoyl-EALAQGPLQ-EDDnp
0.0393
2-aminobenzoyl-EALFQAPLQ-EDDnp
-
in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate
0.0533
2-aminobenzoyl-EALFQGALQ-EDDnp
-
in 50 mM Tris-HCl, at pH 8.0, in the presence of 1 M sodium citrate
0.0185 - 0.0213
2-aminobenzoyl-EALFQGPAQ-EDDnp
0.014 - 0.0203
2-aminobenzoyl-EALFQGPLQ-EDDnp
0.0058
4-(4-dimethylaminophenylazo)benzoic acid-KTSAVLQSGFRKME-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
-
-
0.05078
4-(4-dimethylaminophenylazo)benzoyl-ASFMNQSKVRRFE-5'-[(2-aminoethyl)amino] naphthalene-1-sulfonic acid
YP_007969882
pH 7.0, 37°C, recombinant enzyme
0.00012
5-bromopyridin-3-yl furan-3-carboxylate
-
-
0.00024
5-chloropyridin-3-yl furan-2-carboxylate
-
-
0.8
Ac-DEFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
0.3
Ac-EFQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
1.5
Ac-FQLQ-4-nitroanilide
pH 8.5, temperature not specified in the publication
1.3
Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu
-
-
0.0632
Dabcyl-IEALFQGPPKFRE-Edans
in 50 mM Tris, pH 7.0, at 30°C
0.0825
Dabcyl-RTATVQGPSLDFKE-Edans
in 50 mM Tris, pH 7.0, at 30°C
2.33
N-acetyl-DEFQLQ-4-nitroanilide
-
pH and temperature not specified in the publication
0.25 - 2.6
peptides derived from polyprotein cleavage sites
-
-
-
0.099 - 0.391
SLEVLFQGPVRR
0.037 - 0.087
TENLYFQSGTRR
additional information
additional information
-