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Literature summary for 3.4.22.28 extracted from

  • Leong, L.E.C.; Walker, P.A.; Porter, A.G.
    Human rhinovirus-14 protease 3C (3Cpro) binds specifically to the 5-noncoding region of the viral RNA. Evidence that 3Cpro has different domains for the RNA binding and proteolytic activities (1993), J. Biol. Chem., 268, 25735-25739.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
enterovirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu
-
enterovirus

Organism

Organism UniProt Comment Textmining
enterovirus
-
human rhinovirus
-
enterovirus
-
type 14
-

Purification (Commentary)

Purification (Comment) Organism
as expressed in Escherichia coli enterovirus
as fusion protein enterovirus
recombinant enzyme enterovirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu + H2O represents 2C/3A cleavage site, wild-type and E71D, D85N, K155E mutants, not other mutants enterovirus Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln + Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu
-
?
additional information non-proteolytic function: wild-type and H40D, E71A, C16S mutant enzymes bind specifically to 5'-noncoding region of viral RNA, not D85E or D85N mutant enzymes enterovirus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
enterovirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
enterovirus