Cloned (Comment) | Organism |
---|---|
- |
enterovirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu | - |
enterovirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
enterovirus | - |
human rhinovirus | - |
enterovirus | - |
type 14 | - |
Purification (Comment) | Organism |
---|---|
as expressed in Escherichia coli | enterovirus |
as fusion protein | enterovirus |
recombinant enzyme | enterovirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln-Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu + H2O | represents 2C/3A cleavage site, wild-type and E71D, D85N, K155E mutants, not other mutants | enterovirus | Asp-Ser-Leu-Glu-Thr-Leu-Phe-Gln + Gly-Pro-Val-Tyr-Lys-Asp-Leu-Glu | - |
? | |
additional information | non-proteolytic function: wild-type and H40D, E71A, C16S mutant enzymes bind specifically to 5'-noncoding region of viral RNA, not D85E or D85N mutant enzymes | enterovirus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
enterovirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
enterovirus |