Literature summary for 3.4.22.28 extracted from

Costenaro, L.; Kaczmarska, Z.; Arnan, C.; Janowski, R.; Coutard, B.; Sola, M.; Gorbalenya, A.E.; Norder, H.; Canard, B.; Coll, M.
Structural basis for antiviral inhibition of the main protease, 3C, from human enterovirus 93 (2011), J. Virol., 85, 10764-10773.

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Cloned(Commentary)

Cloned (Comment)	Organism
cloned and expressed in Escherichia coli as a His-tagged fusion protein	Enterovirus B93

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of 3Cpro alone and in complex with the anti-HRV molecules compound (AG7404) and rupintrivir (AG7088) at resolutions of 1.9, 1.3, and 1.5 A is reported. This 20.2-kDa cysteine protease adopts a chymotrypsin-like fold with a catalytic triad, Cys147-His40-Glu71, located in the cleft between the two six-stranded barrels. The residues that form the inhibitor binding pockets are highly conserved among EV 3Cpros, which explains the broad activity of compounds developed initially against a specific virus	Enterovirus B93

Crystallization (Comment)

Organism

crystal structure of 3Cpro alone and in complex with the anti-HRV molecules compound (AG7404) and rupintrivir (AG7088) at resolutions of 1.9, 1.3, and 1.5 A is reported. This 20.2-kDa cysteine protease adopts a chymotrypsin-like fold with a catalytic triad, Cys147-His40-Glu71, located in the cleft between the two six-stranded barrels. The residues that form the inhibitor binding pockets are highly conserved among EV 3Cpros, which explains the broad activity of compounds developed initially against a specific virus

Enterovirus B93

Protein Variants

Protein Variants	Comment	Organism
C147A	inactive mutant	Enterovirus B93

Inhibitors

Inhibitors	Comment	Organism	Structure
ethyl (2E,4S)-4-([(2S)-2-[3-[[(5-methyl-1,2-oxazol-3-yl)carbonyl]amino]-2-oxopyridin-1(2H)-yl]pent-4-ynoyl]amino)-5-[(3R)-2-oxopyrrolidin-3-yl]pent-2-enoate	compound AG7404	Enterovirus B93
rupintrivir	-	Enterovirus B93

Organism

Organism	UniProt	Comment	Textmining
Enterovirus B93	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Enterovirus B93

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ac-RHSVGATLEALFQGPPVYREIKIS-NH2 + H2O	-	Enterovirus B93	Ac-RHSVGATLEALFQ + GPPVYREIKIS-NH2	-	?

Synonyms

Synonyms	Comment	Organism
3C protease	-	Enterovirus B93
3Cpro	-	Enterovirus B93

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Enterovirus B93

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Enterovirus B93