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Literature summary for 3.4.22.28 extracted from

  • Herod, M.R.; Prince, C.A.; Skilton, R.J.; Ward, V.K.; Cooper, J.B.; Clarke, I.N.
    Structure-based design and functional studies of novel noroviral 3C protease chimaeras offer insights into substrate specificity (2014), Biochem. J., 464, 461-472.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Norwalk virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.33
-
 N-acetyl-DEFQLQ-4-nitroanilide pH and temperature not specified in the publication Norwalk virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 x * 18000, SDS-PAGE Norwalk virus

Organism

Organism UniProt Comment Textmining
Norwalk virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-DEFQLQ-4-nitroanilide + H2O
-
 Norwalk virus N-acetyl-DEFQLQ + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 18000, SDS-PAGE Norwalk virus

Synonyms

Synonyms Comment Organism
3C protease
-
 Norwalk virus
NS6 protease
-
 Norwalk virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 N-acetyl-DEFQLQ-4-nitroanilide pH and temperature not specified in the publication Norwalk virus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.034
-
 N-acetyl-DEFQLQ-4-nitroanilide pH and temperature not specified in the publication Norwalk virus