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Literature summary for 3.4.22.28 extracted from

  • Birtley, J.R.; Knox, S.R.; Jaulent, A.M.; Brick, P.; Leatherbarrow, R.J.; Curry, S.
    Crystal structure of foot-and-mouth disease virus 3C protease. New insights into catalytic mechanism and cleavage specificity (2005), J. Biol. Chem., 280, 11520-11527.
    View publication on PubMed

Application

Application Comment Organism
medicine 3Cpro as an attractive target for antiviral drugs Foot-and-mouth disease virus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) pLysS from a pET-28 vector modified to add a thrombin-cleavable N-terminal polyhistidine tag Foot-and-mouth disease virus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion, at 1.9 A resolution Foot-and-mouth disease virus

Protein Variants

Protein Variants Comment Organism
C142S enhanced solubility Foot-and-mouth disease virus
C163A elimination of the activity of the active site nucleophile Foot-and-mouth disease virus
C95K enhanced solubility Foot-and-mouth disease virus

Organism

Organism UniProt Comment Textmining
Foot-and-mouth disease virus
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Foot-and-mouth disease virus A10_61
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-
-

Purification (Commentary)

Purification (Comment) Organism
on TALON resin and by gel filtration on a Hi Load 16/60 Superdex 75 column Foot-and-mouth disease virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Foot-and-mouth disease virus polyprotein + H2O 3Cpro is only weakly selective for Gln over Glu, most susceptible peptides correspond to the VP1/2A and 2B/2C junctions, which were completely cleaved within 6 hours, whereas peptides corresponding to the VP2/VP3, 2C/3A, 3A/3B1, 3B1/3B2 and 3B2/3B3 junctions were uncleaved after 24 hours incubation Foot-and-mouth disease virus ?
-
?
Foot-and-mouth disease virus polyprotein + H2O 3Cpro is only weakly selective for Gln over Glu, most susceptible peptides correspond to the VP1/2A and 2B/2C junctions, which were completely cleaved within 6 hours, whereas peptides corresponding to the VP2/VP3, 2C/3A, 3A/3B1, 3B1/3B2 and 3B2/3B3 junctions were uncleaved after 24 hours incubation Foot-and-mouth disease virus A10_61 ?
-
?

Synonyms

Synonyms Comment Organism
3C protease
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Foot-and-mouth disease virus
3Cpro
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Foot-and-mouth disease virus