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Literature summary for 3.4.22.28 extracted from

  • Shang, L.; Zhang, S.; Yang, X.; Sun, J.; Li, L.; Cui, Z.; He, Q.; Guo, Y.; Sun, Y.; Yin, Z.
    Biochemical characterization of recombinant enterovirus 71 3C protease with fluorogenic model peptide substrates and development of a biochemical assay (2015), Antimicrob. Agents Chemother., 59, 1827-1836.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Enterovirus A71

General Stability

General Stability Organism
changes in salt concentration from 100 mM to 500 mM NaCl produce little effect on the enzymatic activity. Enterovirus A71

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0431
-
 (2-(N-methylamino)benzoyl)-IEALFQGPPK(2,4-dinitrophenyl)-FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0482
-
 2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0582
-
 2-(N-methylamino)benzoyl-RTATVQGPSLDFK-2,4-dinitrophenyl in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0632
-
 Dabcyl-IEALFQGPPKFRE-Edans in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0667
-
 2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0735
-
 2-(N-methylamino)benzoyl-DEAMEQGVSDYIK-2,4-dinitrophenyl in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.075
-
 2-(N-methylamino)benzoyl-TSAVLQSGFRK-(2,4-dinitrophenyl)-Met in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.075
-
 2-(N-methylamino)benzoyl-IAALFQGPPK(2,4-dinitrophenyl)FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0825
-
 Dabcyl-RTATVQGPSLDFKE-Edans in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0924
-
 2-(N-methylamino)benzoyl-RQAVTQGFPTELK-2,4-dinitrophenyl in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl highest proteolytic activity is at 150 mM NaCl Enterovirus A71

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO no significant difference in protease cleavage efficiency is observed with DMSO concentrations of 0.5% to 10% (v/v) Enterovirus A71

Organism

Organism UniProt Comment Textmining
Enterovirus A71 E7E815 Fuyang
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Enterovirus A71

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-(N-methylamino)benzoyl)-IEALFQGPPK(2,4-dinitrophenyl)-FR + H2O most efficient substrate Enterovirus A71 (2-(N-methylamino)benzoyl)-IEALFQ + GPPK(2,4-dinitrophenyl)-FR
-
 ?
2-(N-methylamino)benzoyl-DEAMEQGVSDYIK-2,4-dinitrophenyl + H2O
-
 Enterovirus A71 2-(N-methylamino)benzoyl-DEAMEQ + GVSDYIK-2,4-dinitrophenyl
-
 ?
2-(N-methylamino)benzoyl-IAALFQGPPK(2,4-dinitrophenyl)FR + H2O
-
 Enterovirus A71 2-(N-methylamino)benzoyl-IAALFQ + GPPK(2,4-dinitrophenyl)FR
-
 ?
2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR + H2O
-
 Enterovirus A71 2-(N-methylamino)benzoyl-IEAAFQ + GPPK(2,4-dinitrophenyl)FR
-
 ?
2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR + H2O poor substrate Enterovirus A71 2-(N-methylamino)benzoyl-IEAAFQ + GPPK(2,4-dinitrophenyl)FR
-
 ?
2-(N-methylamino)benzoyl-IEALAQGPPK(2,4-dinitrophenyl)FR + H2O poor substrate Enterovirus A71 2-(N-methylamino)benzoyl-IEALAQ + GPPK(2,4-dinitrophenyl)FR
-
 ?
2-(N-methylamino)benzoyl-IEALFQSPPK(2,4-dinitrophenyl)FR + H2O poor substrate Enterovirus A71 2-(N-methylamino)benzoyl-IEALFQ + SPPK(2,4-dinitrophenyl)FR
-
 ?
2-(N-methylamino)benzoyl-IEALKQGPPK(2,4-dinitrophenyl)FR + H2O poor substrate Enterovirus A71 2-(N-methylamino)benzoyl-IEALKQ + GPPK(2,4-dinitrophenyl)FR
-
 ?
2-(N-methylamino)benzoyl-IEFLFQGPPK(2,4-dinitrophenyl)FR + H2O poor substrate Enterovirus A71 2-(N-methylamino)benzoyl-IEFLFQ + GPPK(2,4-dinitrophenyl)FR
-
 ?
2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR + H2O
-
 Enterovirus A71 2-(N-methylamino)benzoyl-IELLFQ + GPPK(2,4-dinitrophenyl)FR
-
 ?
2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR + H2O poor substrate Enterovirus A71 2-(N-methylamino)benzoyl-IELLFQ + GPPK(2,4-dinitrophenyl)FR
-
 ?
2-(N-methylamino)benzoyl-LFAGFQGAYSGAK-2,4-dinitrophenyl + H2O
-
 Enterovirus A71 2-(N-methylamino)benzoyl-LFAGFQ + GAYSGAK-2,4-dinitrophenyl
-
 ?
2-(N-methylamino)benzoyl-QTGTIQGDRVADK-2,4-dinitrophenyl + H2O poor substrate Enterovirus A71 2-(N-methylamino)benzoyl-QTGTIQ + GDRVADK-2,4-dinitrophenyl
-
 ?
2-(N-methylamino)benzoyl-RQAVTQGFPTELK-2,4-dinitrophenyl + H2O
-
 Enterovirus A71 2-(N-methylamino)benzoyl-RQAVTQ + GFPTELK-2,4-dinitrophenyl
-
 ?
2-(N-methylamino)benzoyl-RTATVQGPSLDFK-2,4-dinitrophenyl + H2O
-
 Enterovirus A71 2-(N-methylamino)benzoyl-RTATVQ + GPSLDFK-2,4-dinitrophenyl
-
 ?
2-(N-methylamino)benzoyl-TSAVLQSGFRK-(2,4-dinitrophenyl)-Met + H2O
-
 Enterovirus A71 2-(N-methylamino)benzoyl-TSAVLQ + SGFRK-(2,4-dinitrophenyl)-Met
-
 ?
Dabcyl-IEALFQGPPKFRE-Edans + H2O
-
 Enterovirus A71 Dabcyl-IEALFQ + GPPKFRE-Edans
-
 ?
Dabcyl-RTATVQGPSLDFKE-Edans + H2O
-
 Enterovirus A71 Dabcyl-RTATVQ + GPSLDFKE-Edans
-
 ?
Dabcyl-TSAVLQSGFRKM-Edans + H2O poor substrate Enterovirus A71 Dabcyl-TSAVLQ + SGFRKM-Edans
-
 ?

Synonyms

Synonyms Comment Organism
3C protease
-
 Enterovirus A71

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Enterovirus A71

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 2-(N-methylamino)benzoyl-TSAVLQSGFRK-(2,4-dinitrophenyl)-Met in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0005
-
 2-(N-methylamino)benzoyl-DEAMEQGVSDYIK-2,4-dinitrophenyl in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.002
-
 Dabcyl-RTATVQGPSLDFKE-Edans in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0027
-
 2-(N-methylamino)benzoyl-IAALFQGPPK(2,4-dinitrophenyl)FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0028
-
 Dabcyl-IEALFQGPPKFRE-Edans in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.003
-
 2-(N-methylamino)benzoyl-RQAVTQGFPTELK-2,4-dinitrophenyl in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0035
-
 2-(N-methylamino)benzoyl-RTATVQGPSLDFK-2,4-dinitrophenyl in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.004
-
 2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.005
-
 2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.0085
-
 (2-(N-methylamino)benzoyl)-IEALFQGPPK(2,4-dinitrophenyl)-FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Enterovirus A71

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0045
-
 2-(N-methylamino)benzoyl-TSAVLQSGFRK-(2,4-dinitrophenyl)-Met in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.007
-
 2-(N-methylamino)benzoyl-DEAMEQGVSDYIK-2,4-dinitrophenyl in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.024
-
 Dabcyl-RTATVQGPSLDFKE-Edans in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.036
-
 2-(N-methylamino)benzoyl-IAALFQGPPK(2,4-dinitrophenyl)FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.037
-
 2-(N-methylamino)benzoyl-RQAVTQGFPTELK-2,4-dinitrophenyl in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.045
-
 Dabcyl-IEALFQGPPKFRE-Edans in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.06
-
 2-(N-methylamino)benzoyl-RTATVQGPSLDFK-2,4-dinitrophenyl in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.075
-
 2-(N-methylamino)benzoyl-IEAAFQGPPK(2,4-dinitrophenyl)FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
0.083
-
 2-(N-methylamino)benzoyl-IELLFQGPPK(2,4-dinitrophenyl)FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71
1.97
-
 (2-(N-methylamino)benzoyl)-IEALFQGPPK(2,4-dinitrophenyl)-FR in 50 mM Tris, pH 7.0, at 30°C Enterovirus A71