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3.4.22.28: picornain 3C

This is an abbreviated version!
For detailed information about picornain 3C, go to the full flat file.

Word Map on EC 3.4.22.28

Reaction

Selective cleavage of Gln-/-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly =

Synonyms

3C protease, 3C protein, 3C proteinase, 3C-like protease, 3C-protease, 3CL, 3cLpro, 3CP, 3Cpro, 3Cpro protease, Avihepatovirus 3C protease, coxsackievirus 3C proteinase, cysteine proteinase 3C, DHAV 3C protease, enterovirus 3C protease, EV71 3Cpro, foot-and-mouth protease 3C, HAC 3C, HAV 3C protease, HAV 3Cpro, hepatitis A virus 3C proteinase, HRV 3C protease, HRV 3Cpro, HRV type-14 3C protease, HRV16 3C protease, Human rhinovirus 3C protease, human rhinovirus type-14 3C protease, nonstructural 3 protease, NS3 protease, NS6 protease, P3C, picornain 3C, picornain-3C, picornaviral 3C protease, picornavirus endopeptidase 3C, poliovirus protease 3C, poliovirus proteinase 3C, protease 3C, proteinase, foot-and-mouth-disease virus, 3C, proteinase, picornavirus, 3C, proteinase, poliovirus, 3C, proteinase, rhinovirus, 3C, rhinovirus 3C protease, rhinovirus protease 3C, SAT-type 3C protease, Senecavirus A 3C protease, SV3CP, SVA 3Cpro, tomato ringspot nepovirus 3C-related protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.28 picornain 3C

Purification

Purification on EC 3.4.22.28 - picornain 3C

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoforms, as expressed in Escherichia coli
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as expressed in Escherichia coli
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as fusion protein
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from Escherichia coli inclusion bodies
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from Escherichia coli membrane fraction
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His-3Cpro is purified using Ni-nitrilotriacetic acid chelating resin under non-denaturing conditions
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HisTrap column chromatography and Superdex 75 gel filtration
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mutant enzyme
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Ni-NTA column chromatography
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Ni-NTA column chromatography and DEAE column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
nickel-chelating column chromatography
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on TALON resin and by gel filtration on a Hi Load 16/60 Superdex 75 column
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Q-Sepharose column chromatography and BioGel hydroxyapatite column chromatography
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recombinant enzyme
recombinant enzyme from Escherichia coli in soluble form
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recombinant enzyme from infected HeLa cells, to near homogeneity
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recombinant enzyme is purified by ion exchange chromatography
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recombinant enzymes
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recombinant enzymes are purified by using a Ni-NTA column
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recombinant His-tagged DHAV 3C protease from Escherichia coli strain BL21 by nickel affinity chromatography
YP_007969882
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS lysed cells by protamin treatment, metal affinity chromatography, thrombin treatment for tag cleavage, and dialysis followed by gel filtration
recombinant protein purified to 90% homogeneity
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recombinant soluble GST-tagged enzyme by glutathione affinity chromatography from Escherichia coli strain BL21(DE3), method optimization for large-scale purification
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Resource-Q column chromatography and a Superdex-75 gel filtration
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soluble mutant proteins are purified to greater than 80% purity in a single step by using a nickel nitrilotriacetic acid agarose spin column
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Talon beads chromatography
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TALON resin chromatography and by gel filtration
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to near homogeneity
using a DEAE column
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using Ni-NTA chromatography
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using the IMPACT-CN system
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wild-type and 3 mutant enzymes
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