1.1.1.2: alcohol dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about alcohol dehydrogenase (NADP+), go to the full flat file.

Reaction

a primary alcohol
+
NADP+
=
an aldehyde
+
NADPH
+
H+

Synonyms

2° Adh, 3-DG-reducing enzyme, AAur_2040, ADH, ADH-1, ADH-2, ADH-I, ADH-II, ADH2, Adh319, ADH4, Adh6, AdhA, AdhB, AKR1A1, AKR1A4, alcohol dehydrogenase C, alcohol dehydrogenase [NADP(+)], Alcohol dehydrogenase [NADP+], aldehyde reductase, aldehyde reductase (NADPH2), aldehyde/ketone reductase, aldo-keto reductase, Aldo-keto reductase family 1 member A1, aldose reductase, ALDR, alipathic aldehyde reductase, ALR, ALR 1, ALR1, BdhA, bovine brain aldehyde reductase, D-glucuronate reductase, daunorubicin reductase, DRD, EhADH1, Gre2p, hexogenate dehydrogenase, high-Km aldehyde reductase, HvADH2, L-hexonate dehydrogenase, LB-RADH, LBADH, liver alcohol dehydrogenase, low-Km aldehyde reductase, mevaldate reductase, Mpd1, NADP(H)-dependent alcohol dehydrogenase, NADP-alcohol dehydrogenase, NADP-aldehyde reductase, NADP-dependent aldehyde reductase, NADP-linked aryl alcohol dehydrogenase, NADPH-aldehyde reductase, NADPH-cytochrome c reductase, NADPH-dependent aldehyde reductase, NADPH-dependent FALDR, NADPH-dependent fatty aldehyde reductase, NADPH-linked aldehyde reductase, nonspecific succinic semialdehyde reductase, Octopine dehydrogenase, putative iron alcohol dehydrogenase, PyAeADHII, rabbit kidney aldehyde-ketone reductase, RADH, ScADHVI, short-chain ADH, short-chain alcohol dehydrogenase, short-chain dehydrogenase/reductase, TBADH, TbADH1, Teth39_1597, Teth514_0564, TPN-L-hexonate dehydrogenase, TPNH-linked aldehyde reductase, TPNH-specific aldehyde reductase, triphosphopyridine nucleotide-linked aldehyde reductase, TsAdh319, Y63 protein, yeast alcohol dehydrogenase, YqhD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.2 alcohol dehydrogenase (NADP+)

Storage Stability

Storage Stability on EC 1.1.1.2 - alcohol dehydrogenase (NADP+)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C AR1 stable for at least 6 months
-
-20°C AR3 loses most of its activity
-
-20°C can be stored in 20% glycerol/10 mM phosphate buffer, pH 8, 0.5 mg/ml without any loss of activity for 1 month
-
-20°C, 20% v/v glycerol, stable for at least one month
-
-20°C, stable for several months if stored as an ammonium sulfate precipitate
-
0-2°C, enzyme activity retained for up to 3 months if frozen immediately after preparation, stable for several days, freezing and thawing is quite detrimental to retention of enzyme activity
-
0-4°C stable for 2 or 3 weeks in 44 mM sodium phosphate buffer, pH 7
-
0-4°C stable for 2-3 months as an (NH4)2SO4 precipitate
-
0°C stable in the frozen state for 3-4 months without any appreciable loss in activity
-
0°C when stored frozen there is negligible loss of activity after 6 months, but repeated freezing and thawing results in loss of activity
-
0°C, freezing of the purified enzymes results in almost complete loss of activity, purified aldehyde reductase II is stable in 10 mM sodium phosphate buffer, pH 7, for 2-3 month, but appears to lose activity rapidly thereafter, purified aldehyde reductase I retains enzyme activity in the same buffer for about 3 months, after which enzyme activity declines
-
1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, 51% and 46% residual activity, respectively
-
1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, quite stable
-
20°C reductase I stable for more than 1 month
-
23°C, loses all of its activity within 1 week when stored anaerobically
-
4°C AR1 loses all activity after storage for 1 month
-
4°C AR3 stable for at least 1 month in presence of NaN3
-
4°C half-life 6-7 days when stored aerobically
-
4°C in 0.01% NaN3 stable for at least 1 month, the gel-filtered enzyme is stable for approximately 2 weeks
-
4°C loses activity only slowly over several weeks
-
4°C purified enzymes are stable for at least 2 days, then gradually loses activity during storage
-
4°C reductase I and II are stable for several months
-
4°C stable for several months but loss of activity with a concomitant enzyme precipitation occurs upon freezing and thawing
-
4°C, 6 mg/ml, stable for several months with 10 mM mercaptoethanol
-
4°C, about 1 week, 50% activity lost
-
4°C, extremely unstable, 20% ethylene glycol stabilizes
-
4°C, several months, stable