1.1.1.2: alcohol dehydrogenase (NADP+)

This is an abbreviated version, for detailed information about alcohol dehydrogenase (NADP+), go to the full flat file.

Reaction

a primary alcohol
+
NADP+
=
an aldehyde
+
NADPH
+
H+

Synonyms

2 Adh, 3-DG-reducing enzyme, AAur_2040, ADH, ADH-1, ADH-2, ADH-I, ADH-II, ADH2, Adh319, ADH4, Adh6, AdhA, AdhB, AKR1A1, AKR1A4, alcohol dehydrogenase C, alcohol dehydrogenase [NADP(+)], Alcohol dehydrogenase [NADP+], aldehyde reductase, aldehyde reductase (NADPH2), aldehyde/ketone reductase, aldo-keto reductase, Aldo-keto reductase family 1 member A1, aldose reductase, ALDR, alipathic aldehyde reductase, ALR, ALR 1, ALR1, BdhA, bovine brain aldehyde reductase, D-glucuronate reductase, daunorubicin reductase, DRD, EhADH1, Gre2p, hexogenate dehydrogenase, high-Km aldehyde reductase, HvADH2, L-hexonate dehydrogenase, LB-RADH, LBADH, liver alcohol dehydrogenase, low-Km aldehyde reductase, mevaldate reductase, Mpd1, NADP(H)-dependent alcohol dehydrogenase, NADP-alcohol dehydrogenase, NADP-aldehyde reductase, NADP-dependent aldehyde reductase, NADP-linked aryl alcohol dehydrogenase, NADPH-aldehyde reductase, NADPH-cytochrome c reductase, NADPH-dependent aldehyde reductase, NADPH-dependent FALDR, NADPH-dependent fatty aldehyde reductase, NADPH-linked aldehyde reductase, nonspecific succinic semialdehyde reductase, Octopine dehydrogenase, putative iron alcohol dehydrogenase, PyAeADHII, rabbit kidney aldehyde-ketone reductase, RADH, ScADHVI, short-chain ADH, short-chain alcohol dehydrogenase, short-chain dehydrogenase/reductase, TBADH, TbADH1, Teth39_1597, Teth514_0564, TPN-L-hexonate dehydrogenase, TPNH-linked aldehyde reductase, TPNH-specific aldehyde reductase, triphosphopyridine nucleotide-linked aldehyde reductase, TsAdh319, Y63 protein, yeast alcohol dehydrogenase, YqhD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.2 alcohol dehydrogenase (NADP+)

Specific Activity

Specific Activity on EC 1.1.1.2 - alcohol dehydrogenase (NADP+)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.008
-
low-Km reductase
0.032
Moorella sp.
-
isoform AdhA, oxidation of ethanol, cofactor NAD+, pH 8.0, 60C
0.049
Moorella sp.
-
isoform AdhA, reduction of acetaldehyde, cofactor NADH, pH 8.0, 60C
0.05
-
-
0.06
-
mutant enzyme N86D/R213I, using 2-pentanol as substrate, at 30C
0.07
-
mutant enzyme N86D/R213I, using 2-propanol as substrate, at 30C; mutant enzyme N86D/R213I, using (2S,5S)-hexanediol as substrate, at 30C
0.08
-
mutant enzyme K209T, using 2-hexanedione as substrate, at 30C
0.085
-
highest specific activity for the reduction of decanal
0.09
-
mutant enzyme N86D/R213I, using 2-butanol as substrate, at 30C; wild type enzyme, using 2-hexanedione as substrate, at 30C
0.11
-
wild type enzyme, using 2-propanol as substrate, at 30C
0.12
-
mutant enzyme A18P/K40N/Q165H/D182H/E202G/R219M, using 2-hexadione as substrate, at 30C; mutant enzyme N86D/R213I, using 2-hexanedione as substrate, at 30C
0.14
-
mutant enzyme V66A/L176P/Y229H, using 2-hexanedione as substrate, at 30C
0.15
-
mutant enzyme K209T, using 2-propanol as substrate, at 30C
0.16
-
wild type enzyme, using 2-butanol as substrate, at 30C
0.17
-
mutant enzyme T153A, using 2-propanol as substrate, at 30C
0.18
-
mutant enzyme T153A, using 2-hexanedione as substrate, at 30C
0.2
-
mutant enzyme A18P/K40N/Q165H/D182H/E202G/R219M, using 2-propanol as substrate, at 30C; mutant enzyme L176P, using 2-hexanedione as substrate, at 30C
0.24
-
mutant enzyme T153A, using 2-butanol as substrate, at 30C
0.27
-
with 4-methoxybenzaldehyde as substrate
0.33
-
mutant enzyme V66A/L176P/Y229H, using 2-butanol as substrate, at 30C
0.34
-
high-Km reductase
0.35
-
mutant enzyme K209T, using 2-butanol as substrate, at 30C
0.3786
-
-
0.42
-
activiy of ALR in Sporobolomyces salmonicolor, pH 6.5, assay mixture including potassium phosphate buffer, NADPH and p-nitrobenzaldehyde
0.43
-
mutant enzyme L176P, using 2-propanol as substrate, at 30C
0.55
-
AR3
0.57
-
mutant enzyme L176P, using 2-butanol as substrate, at 30C
0.58
-
with 5-(hydroxymethyl)furan-2-carbaldehyde as substrate
0.6
-
mutant enzyme A18P/K40N/Q165H/D182H/E202G/R219M, using 2-butanol as substrate, at 30C
0.65
-
mutant enzyme K209T, using 2-pentanol as substrate, at 30C
0.7
-
coexpression of formate dehydrogenase and alcohol dehydrogenase reducing methyl acetoacetate to methyl (R)-3-hydroxy butanoate with NADH, photometrically at 340 nm in a mixture of 10mM methyl acetoacetate, 250 microM NADH, 1 mM MgCl2, in 100 mM potassium phosphate buffer, pH 6.0
0.71
-
mutant enzyme A18P/K40N/Q165H/D182H/E202G/R219M, using (2S,5S)-hexanediol as substrate, at 30C
0.73
-
mutant enzyme L176P, using 2-pentanol as substrate, at 30C; wild type enzyme, using pyruvaldehyde as substrate, at 30C
0.78
-
mutant enzyme N86D/R213I, using pyruvaldehyde as substrate, at 30C
0.86
-
mutant enzyme T153A, using 2-pentanol as substrate, at 30C
0.9
-
with acetaldehyde as substrate
0.91
-
wild type enzyme, using (2S,5S)-hexanediol as substrate, at 30C
0.95
-
mutant enzyme R11L/A180V, using 2-hexanedione as substrate, at 30C
0.99
-
with trans-2-nonenaldehyde as substrate
1.1
-
mutant enzyme K209T, using (2S,5S)-hexanediol as substrate, at 30C
1.12
-
mutant enzyme K209T, using pyruvaldehyde as substrate, at 30C
1.22
-
mutant enzyme V66A/L176P/Y229H, using pyruvaldehyde as substrate, at 30C
1.24
-
mutant enzyme L176P, using (2S,5S)-hexanediol as substrate, at 30C
1.27
substrate formaldehyde, cofactor NADPH, pH 7.2, 30C
1.28
-
with cinnamic aldehyde as substrate
1.47
-
wild type enzyme, using 2-pentanol as substrate, at 30C
1.5
-
-
1.98
-
mutant enzyme R11L/A180V, using 2-butanol as substrate, at 30C
2.5
-
-
2.9
Moorella sp.
-
isoform AdhB, reduction of acetaldehyde, cofactor NADPH, pH 8.0, 60C
3
-
mutant enzyme T153A, using pyruvaldehyde as substrate, at 30C
3.23
-
mutant enzyme R11L/A180V, using (2S,5S)-hexanediol as substrate, at 30C
3.37
-
mutant enzyme A18P/K40N/Q165H/D182H/E202G/R219M, using pyruvaldehyde as substrate, at 30C
3.47
-
mutant enzyme R11L/A180V, using 2-pentanol as substrate, at 30C
3.63
-
with octanaldehyde as substrate
3.73
-
with heptanaldehyde as substrate
3.97
-
with phenylacetaldehyde as substrate
4.19
-
with with furan-2-carbaldehyde as substrate
4.8
-
pyridine 3-aldehyde as substrate
4.99
-
with valeraldehyde as substrate
5.6
native enzyme from cell-free extract, in 100 mM EPPS buffer (pH 8.8)
5.86
-
AR2
5.9
-
liver enzyme
6
-
brain enzyme
6.34
-
with butyraldehyde as substrate
6.41
-
with propionaldehyde as substrate
6.48
-
activity of ALR in Escherichia coli M15, the enzyme activity is fifteen times higher than thatof Sporobolomyces salmonicolor ZJUB 105
6.5
-
coexpression of formate dehydrogenase and alcohol dehydrogenase, reducing methyl acetoacetate to methyl (R)-3-hydroxy butanoate with NADPH, photometrically at 340 nm in a mixture of 10 mM methyl acetoacetate, 250 microM NADPH, 1 mM MgCl2, in 100 mM potassium phosphate buffer, pH 6.0
6.57
-
activiy of ALR in Eschericher coli M15 with the plasmid pQE30-alr0307, pH 6.5, assay mixture including potassium phosphate buffer, NADPH and p-nitrobenzaldehyde
6.66
-
native enzyme
6.8
recombinant enzyme from cell-free extract, in 100 mM EPPS buffer (pH 8.8)
7.67
-
with hexanaldehyde as substrate
7.97
-
mutant enzyme R11L/A180V, using pyruvaldehyde as substrate, at 30C
8.1
-
cell-free protein
8.94
-
AR1
9.59
-
high-Km aldehyde reductase from brain
11.9
Moorella sp.
-
isoform AdhA, oxidation of ethanol, cofactor NADP+, pH 8.0, 60C
12.22
substrate acetaldehyde, cofactor NADPH, pH 7.2, 30C
14.3
-
pure aldehyde reductase
15.56
substrate acetaldehyde, cofactor NADH, pH 7.2, 30C
15.7
-
-
17.1
-
-
18.8
-
-
19.7
-
AR1
19.9
-
AR3
24
-
recombinant enzyme
29.62
-
aldehyde reductase II
29.7
-
substrate ethanol, isoform ADH-1, pH 8.8, 55C
30.06
-
substrate propan-1-ol, isoform ADH-1, pH 8.8, 55C
34.06
-
substrate octan-1-ol, isoform ADH-1, pH 8.8, 55C
37.32
-
substrate pentan-1-ol, isoform ADH-1, pH 8.8, 55C
40.04
-
substrate hexan-1-ol, isoform ADH-1, pH 8.8, 55C
50.4
Moorella sp.
-
isoform AdhA, reduction of acetaldehyde, cofactor NADPH, pH 8.0, 60C
52.8
recombinant enzyme after 7.8fold purification, in 100 mM EPPS buffer (pH 8.8)
58
-
substrate octan-1-ol, isoform ADH-2, pH 8.8, 55C
72.3
-
80C, pH not specified in the publication
90
-
pH 7.0, 25C
104.2
-
substrate pentan-1-ol, isoform ADH-2, pH 8.8, 55C
116.2
-
substrate hexan-1-ol, isoform ADH-2, pH 8.8, 55C
183
-
25C, pH 7.0
211
-
hexadecanal as substrate
additional information